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1

Overexpression of 4.1R disturbs microtubule organization

100%

Perez-Ferreiro C. M., Luque C. M., Perez-Gonzales A., Sendino E., Correas I.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 2

2

Involvement of microtubules in the transport of the secretion in secretory cells of the mammary gland

88%

Sporniak M., Perissel B.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 4

3

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Alisma plantago-aquatica L.: the cytoskeleton of the suspensor development

75%

Swierczynska J., Bohdanowicz J.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 2

The actin and the tubulin cytoskeleton organization during the differentiation of the embryo-suspensor in Alisma plantago-aquatica was studied in comparison with the development of embryo, using immunofluorescence detection and rhodamine-phalloidin assay. At the early stage of the suspensor basal cell development (from 2- to ~10-celled embryos) microfilaments form an abundant network in the cytoplasm of the basal cell, while the microtubules form a delicate network. At the mature stage of development (from a dozen to several dozen-celled embryos), in the suspensor basal cell, the microfilaments and microtubules were localized from micropylar to chalazal pole of the cell. At the micropylar end of the basal cell a high amount of actin and tubulin material was observed. The microfilaments were mainly arranged parallel whereas numerous bundles of microtubules distributed longitudinally or transversally to the long axis of the cell. At this stage of basal cell functioning, some bundles of microtubules appeared to pass close to the nucleus surface. Microtubules were also observed distributed at the chalazal pole of the basal cell. At the senescence stage of the suspensor basal cell (>100-celled embryos) the actin and tubulin filaments disorganize, some disrupted microfilaments and microtubules were observed in the cytoplasm of the basal cell. At all stages of the suspensor basal cell development in the embryo cells an extensive actin and tubulin network was observed.

4

Dynamic instability - A common denominator in prokaryotic and eukaryotic DNA segregation and cell division

75%

Fuesler J. A., Li H.-J. S.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 4

Dynamic instability is an essential phenomenon in eukaryotic nuclear division and prokaryotic plasmid R1 segregation. Although the molecular machines used in both systems differ greatly in composition, strong similarities and requisite nuances in dynamics and segregation mechanisms are observed. This brief examination of the current literature provides a functional comparison between prokaryotic and eukaryotic dynamically unstable filaments, specifically ParM and microtubules. Additionally, this mini-review should support the notion that any dynamically unstable filament could serve as the molecular machine driving DNA segregation, but these machines possess auxiliary features to adapt to temporal and spatial disparities in either system.

5

p600 stabilizes microtubules to prevent the aggregation of CaMKIIalpha during photoconductive stimulation

75%

Belzil C., Ramos T., Sanada K., Colicos M. A., Nguyen M. D.

Cellular and Molecular Biology Letters

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2014

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tom 19

|

nr 3

The large microtubule-associated/Ca2+-signalling protein p600 (also known as UBR4) is required for hippocampal neuronal survival upon Ca2+ dyshomeostasis induced by glutamate treatment. During this process, p600 prevents aggregation of the Ca2+/calmodulin-dependent kinase IIα (CaMKIIα), a proxy of neuronal death, via direct binding to calmodulin in a microtubuleindependent manner. Using photoconductive stimulation coupled with live imaging of single neurons, we identified a distinct mechanism of prevention of CaMKIIα aggregation by p600. Upon direct depolarization, CaMKIIα translocates to microtubules. In the absence of p600, this translocation is interrupted in favour of a sustained self-aggregation that is prevented by the microtubule-stabilizing drug paclitaxel. Thus, during photoconductive stimulation, p600 prevents the aggregation of CaMKIIα by stabilizing microtubules. The effectiveness of this stabilization for preventing CaMKIIα aggregation during direct depolarization but not during glutamate treatment suggests a model wherein p600 has two modes of action depending on the source of cytosolic Ca2+.

6

Immunocytochemical analysis of alpha-tubulin distribution before and after rapid axopodial contraction in the centrohelid Raphidocystis contractilis

75%

Ikeda R., Kurokawa M., Murai M., Saito N., Ando M.

Acta Protozoologica

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2020

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tom 59

|

nr 1

7

Analysis of water-soluble proteins by two-dimensional electrophoresis in the encystment process of Colpoda cucullus Nag-1 and cytoskeletal dynamics

75%

Sogame Y., Kojima K., Takeshita T., Kikuchi S., Shimada Y., Nakamura R., Arikawa M., Miyata S., Kinoshita E., Suizu F., Matsuoka T.

Acta Protozoologica

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2020

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tom 59

|

nr 3-4

8

Cyclin-dependent kinase inhibition by olomoucine analogues leads to abnormal spindle formation in higher plant cells

75%

Binarova P., Bogre L., Hirt H., Haberle-Bors E., Strnad M.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

9

Submicroscopic studies on adrenaline secretion from adrenal epinephrocytes

75%

Kmiec B. L.

Folia Morphologica

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1992

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tom 51

|

nr 1

10

Morphologie et ultrastructure du cilie Condylostomides grolieri gen.n., sp.n. [Ciliophora: Heterotrichida]

75%

Silva Neto I. D.

Acta Protozoologica

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1994

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tom 33

|

nr 3

11

The incorporation of 3H-palmitic acid into Ornithogalum umbellatum lipotubuloids, which are a cytoplasmic domain rich in lipid bodies and microtubules. Light and EM autoradiography

75%

Kwiatkowska M.

Acta Societatis Botanicorum Poloniae

|

2004

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tom 73

|

nr 3

In the ovary epidermis of Ornithogalum umbellatum L. lipotubuloids were found, i.e. distinguished cytoplasmic domain with an agglomeration of half unit membrane-surrounded lipid bodies, entwined and held together by a system of microtubules (Protoplasma 75: 345-357; 77: 473-476). Using light and EM-autoradiography with 3H-palmitic acid (25 μCi/ml) it was found that lipotubuloids were the site of intense incorporation of this isotope. After extraction of lipids with lipid solvent the lipotubuloids were not labeled. Localization of autoradiographic grains after 15-h postincubation with isotope-free medium indicated a migration of the labeled substances from the lipotubuloids to the whole cells. Ultrastructural studies demonstrated that most autoradiographic grains after 2-h incubation were localized over the site of the microtubules adjoining closely the half unit membranes of lipid bodies. These observations suggest, that the surface of lipid bodies may be the active site in lipid synthesis and involvement of the microtubules in these processes is possible.

12

The effect of pendimethalin on plant microtubules

75%

Tarkowska J. A., Kossut E., Dobrzynska K.

Fragmenta Floristica et Geobotanica

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1994

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tom 39

|

nr 1

13

The effect of triethyllead on the motile activity of Walker 256 carcinosarcoma cells

75%

Sroka J., Kaminski R., Michalik M., Madeja Z., Przestalski S., Korohoda W.

Cellular and Molecular Biology Letters

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2004

|

tom 09

|

nr 1

The effect of triethyllead (TriEL) on the morphology and motile activity of Walker 256 carcinosarcoma cells was investigated. It was found that both 2 and 5 μM TriEL affected the cellular motility in a dose- and time- dependent manner. Initially, 2 μM TriEL caused the formation of blebs instead of lamellipodia at the front of some cells and stimulated the migration of Walker cells, but after 2 hours of 2 μM TriEL treatment, a reduction of cellular motility was observed. In the presence of 5 μM TriEL, Walker 256 carcinosarcoma cells rounded up, and their rate of movement was reduced. Moreover, the treatment of Walker carcinosarcoma cells with TriEL caused the disruption of microtubules and affected the F-actin distribution at both concentrations. At a concentration of 2 μM TriEL, the actin staining intensity was greatest in the tail of front-tail polarised blebbing cells and the actin layer was very thin at the leading edge. The control cells showed linear cortical F-actin distribution and somewhat less intense cytoplasmic staining at the same TriEL concentration. Cells treated with 5 μM TriEL showed an under-membrane pattern of actin distribution.

14

Early stages of somatic embryogenesis in sunflower

75%

Pietrusiewicz J., Souvre A., XuHan X., Alibert G., Bednara J.

Acta Physiologiae Plantarum

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1997

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tom 19

|

nr 2

15

The cytoskeleton proteins and Prl-regulated steroidogenesis of porcine theca cells

75%

Gregoraszczuk E. L., Stoklosowa S.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

|

nr 2

16

Cellular organelle transport and positioning by plasma streaming

75%

Wanka F., Van Zoelen E. J. J.

Cellular and Molecular Biology Letters

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2003

|

tom 08

|

nr 4

Our analysis of known data reveals that translocations of passively movable cellular organelles from tiny granules up to large cell nuclei can be ascribed to transport by streaming cytoplasm. The various behaviours, such as velocity changes during more or less interrupted movements, forth and back shuttling and particle rotation result from different types of plasma circulation. Fast movements over long distances, as observed in the large characean internodial cells occur in strong streams generated by myosin in bundles of actin filaments in the direction of the barbed filament ends. Slow movements with frequent reversions of the direction are typical for neuronal axons, in which an anterograde plasma flow, produced in a thin layer of membrane-attached actin filaments, is compensated by a retrograde stream, produced by dynein activity in the central bundle of microtubules. Here particle rotation is due to steep flow velocity gradients, and frequent changes of particle movements result from minor particle displacements in radial directions. Similar shuttling of pigment granules in the lobes of epidermal chromatophores results from the same mechanism, whereby the centrifugal movement along astral microtubules is due to flow generated by excess of kinesin activity and the centripetal movement to the plasma recycling through the intermicrotubular space. If the streaming pattern is reversed by switching to excess dynein activity, the moving granules are trapped in the high microtubule density at the aster center. The presence of larger bodies in asters disturbs the regular, kinesin-dependent microtubule distribution in such a way that a superimposed centrifugal plasma flow develops in the microtubule-dense layer along them, which is recycled in the microtubule-free space, created by their presence. Consequently, at excess kinesin activity, nuclei, mitochondria as well as chromosome fragments move towards the aster center until they reach a dynamically stabilized position that depends on the local microtubule density. These various behaviours are not rationally explainable by models based on a mechanical stepping along microtubules or actin filaments.

17

Structural and physiological basis of axopodial dynamics

75%

Febvre-Chevalier C., Febvre J.

Acta Protozoologica

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1993

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tom 32

|

nr 4

18

Microtubules with different diameter, protofilament number and protofilament spacing in Ornithogalum umbellatum ovary epidermis cells

75%

Kwiatkowska M., Poplonska K., Stepinski D., Hejnowicz Z.

Folia Histochemica et Cytobiologica

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2006

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tom 44

|

nr 2

19

DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules

63%

Kwiatkowska M., Stepinski D., Poplonska K., Wojtczak A., Polit J. T.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 3

20

'Elaioplasts' identified as lipotubuloids in Althaea rosea, Funkia sieboldiana and Vanilla planifolia contain lipid bodies connected with microtubules

63%

Kwiatkowska M., Stepinski D., Poplonska K., Wojtczak A., Polit J.

Acta Societatis Botanicorum Poloniae

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2011

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tom 80

|

nr 3

"Elaioplasts" observed in Vanilla planifolia, Funkia Sieboldiana and Althaea rosea exhibit all the features characteristic of lipotubuloids earlier described in Ornithogalum umbellatum. They are cytoplasmic domains containing aggregates of lipid bodies connected with microtubules. The immunogold technique confirmed the presence of tubulin in this domain. These structures do not have their own membranes but they are surrounded by a tonoplast at the side of a vacuole since they invaginate into it. In cytoplasm of this domain among lipid bodies there are numerous ribosomes, ER cisternae and vesicles as well as few mitochondria, Golgi structures and microbodies while at older developmental stages there are also autolytic vacuoles. The fact that they are so similar to O. umbellatum lipotubuloids suggest that "elaioplasts" of V. planifolia, F. Sieboldiana and A. rosea can also be named lipotubuloids.

Ograniczanie wyników

Overexpression of 4.1R disturbs microtubule organization

Involvement of microtubules in the transport of the secretion in secretory cells of the mammary gland

Alisma plantago-aquatica L.: the cytoskeleton of the suspensor development

Dynamic instability - A common denominator in prokaryotic and eukaryotic DNA segregation and cell division

p600 stabilizes microtubules to prevent the aggregation of CaMKIIalpha during photoconductive stimulation

Immunocytochemical analysis of alpha-tubulin distribution before and after rapid axopodial contraction in the centrohelid Raphidocystis contractilis

Analysis of water-soluble proteins by two-dimensional electrophoresis in the encystment process of Colpoda cucullus Nag-1 and cytoskeletal dynamics