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Microsporidia are intracellular parasites that cause opportunistic infections in humans of various immunological status. Only a few case reports exist on microsporidial infection in solid organ transplant recipients worldwide. The presented study demonstrates the first case in Poland of Enterocytozoon bieneusi infection in a liver transplant patient. Parasites were diagnosed in stool samples using both modified trichrome staining and PCR.
The microsporidium Nosema pontogammari Ovcharenko and Kurandina, 1987 was detected in the muscles of Pontogammarus robustoides collected in the stream of South Baltic Coastal Zone (sample site N 54°16', E 19°25') The parasite infects the cytoplasm of the muscle cells. The presence of remarkable intracytoplasmic vacuoles was noted inside of some parasite cells. Transmission electron microscopy showed that these intracytoplasmic vacuoles contained numerous microorganisms identified as rickettsialike prokaryotic cells. Groups of pleiomorphic in shape bacilliform and rounded cells were enveloped in electron-dense cover 20-30 nm wide. The prokaryotic cells wali was constructed out of triple layered coat of different electron density. The cytoplasm of infected cells was degenerated with weakly developed endoplasmic reticulum and undeveloped organelles. The obtained data demonstrate pathogenic nature of host-parasite relationship between endosymbiotic rickettsia-like organism and microsporidia.
This cross-sectional study was carried to determine the prevalence of intestinal parasitic infections among mentally handicapped individuals in Alexandria, Egypt, in the period from December 2012 till November 2013. The study was conducted on 200 institutionalized and non-institutionalized mentally handicapped individuals. Fresh stool samples were subjected to different stains including; trichrome for detecting intestinal protozoa, modified acid fast stain for intestinal coccidia and quick hot gram chromotrope stain for Microsporidia. Also they were processed by Kato-Katz and formol ethyl acetate techniques for intestinal helminths. Additionally, blood samples were collected for measuring hemoglobin levels. Out of 200 mentally handicapped individuals, 87 (43.5%) were infected. The infection rates were 44.6% and 42.6% for non-institutionalized and institutionalized people, respectively. Regarding gender, 46.7% and 38.5% were reported for the males and females respectively. The most common parasites detected were: Cryptosporidium sp. (23.5%), microsporidia (15%), Giardia lamblia (8.5%), Dientamoeba fragilis (8%), Cyclospora cyatanensis (7.5%), Blastocystis hominis (6.5%), Entamoeba histolytica (5.5%) and Entamoeba coli (2.5%). Rates for Isospora belli and Enterobius vermicularis were estimated to be 1.5% for each, while lower rate was reported for Iodamoeba butschlii (1.0%). Prevalence of infections among mentally handicapped individuals are indications for several risk factors, including improper sanitary hygiene and illiteracy about personal hygiene. Therefore, frequent investigations, health care and medical intervention are needed.
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