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Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc

100%

Talapatra S. N., Banerjee P., Mukhopadhyay A.

International Letters of Natural Sciences

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2014

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tom 04

As far as the detection of metal genotoxicity in fish is concerned, micronucleus (MN) test is considered an extremely suitable measure. In this study, frequencies of micronucleated erythrocytes were scored in peripheral blood of catfish, Heteropneustes fossilis (bloch) after acute in-vivo exposure of zinc at different concentrations (5, 10 and 30 ppm) in the laboratory condition. These three concentrations of zinc were tested at different durations such as 24h, 48h, 72h and 96h respectively. Highly significant (P < 0.001) increased values were obtained for MN frequencies in the peripheral erythrocytes of exposed fishes compared to control groups of fishes. These results confirm that dose- and time-dependent micronucleation in the peripheral erythrocytes of fish after short-term exposure to zinc could provide valuable information regarding zinc containing effluent quality and also help in genetic biomonitoring with this test model. In this context safe concentration of zinc vis-a-vis genotoxicity range could be evaluated for future studies.

2

Genotoxic and haematological effect of commonly used fungicide on fish Clarias batrachus

100%

Shahi J., Singh A.

Journal of Biology and Earth Sciences

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2014

|

tom 04

|

nr 2

3

Estimation of cytogenetic risk among coke oven workers exposed to polycyclic aromatic hydrocarbons

100%

Sureshkumar S., Balachandar V., Devi S. M., Arun M., Karthickkumar A., Balamuralikrishnan B., Sankar K., Mustaqahamed S. A. K., Dharwadkar S. N., Sasikala K., Cho S.-G.

Acta Biochimica Polonica

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2013

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tom 60

|

nr 3

Polycyclic aromatic hydrocarbons (PAHs) result from the incomplete combustion of natural or synthetic organic materials. The working environment at a coke plant can negatively affect the employed workers who were exposed to coke oven emissions containing PAHs, which formed and released into the environment by the process of pyrolysis of coke. This study aims to analyze the relationship between the exposure of PAHs and the risk of genetic damages such as chromosomal alteration (CA), micronucleus (MN), and DNA damage (PCR-RFLP) in peripheral blood lymphocytes of 27 coke oven workers and equal number of control subjects. The exposed subjects and controls were divided into two groups based on their age (group I<35 years and group II ≥35 years). The exposed subjects were further classified into two groups based on the exposure period (<12 years and ≥12 years). The frequencies of CA and MN in exposed subjects are relatively high with respect to controls. The XRCC1 399 Arg/gln polymorphism showed a substantial smaller difference in allele frequencies between exposed and control subjects. Based on present data, it was concluded that coke oven workers under risk should be monitored for adverse effects of the any long-term exposure.

4

Induction of micronuclei in rat bone marrow after subchronic inhalation exposure to mixture of benzene, cyclohexanone and cyclohexane

100%

Kovalkovicova N., Sutiakova I., Legath J., Legath L., Falis M., Pistl J., Kovac G.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

|

nr 3

5

Frequency of micronuclei in mouse peripheral blood erythrocytes following WR-2721 application and X-irradiation

100%

Mazur L.

Folia Biologica

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1995

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tom 43

|

nr 3-4

6

Ultrastructural peculiarities of the meiotic prophase in the ciliate Tracheloraphis totevi [Karyorelictida]: synaptonemal complexes and sheaths around bivalents

100%

Kovaleva V,G., Raikov I. B.

Acta Protozoologica

|

1992

|

tom 31

|

nr 3

7

Cytoplasmic zones responsible for differentiation of the new macronucleus and degradation of the old in Chilodonella steini (Trithigmostoma steini)

84%

Krawczyk M., Golembiewska-Skoczylas M., Radzikowski S.

Acta Protozoologica

|

1990

|

tom 29

|

nr 1

8

Boric acid as a protector against paclitaxel genotoxicity

84%

Turkez H., Tatar A., Hacimuftuoglu A., Ozdemir E.

Acta Biochimica Polonica

|

2010

|

tom 57

|

nr 1

95-97

Paclitaxel (PAC) is an anticancer drug used for treatments of breast, ovarian and lung cancers. However, little data is; available in the literature on its potential genotoxicity on healthy human cells. On the other hand, boron deficiency and supplementation exert important biological effects in human and animal tissues. The biological effects of dietary boron are defined, but its interaction with PAC is not known for therapeutic uses. The aim of the present study was to determine whether boric acid (BA) confer a protection against PAC genotoxicity. After the application of PAC (10 or 20 μg/l) and BA (2.5 or 5 mg/l), the genotoxic effects were assessed by sister chromatid exchange (SCE) and micronucleus (MN) tests in human blood cultures. We also analyzed nuclear division index (NDI) in peripheral lymphocytes. Our results showed that PAC significantly (P < 0.05) increased the frequencies of SCEs and the formations of MNs in peripheral lymphocytes as compared to controls. PAC decreased the nuclear division index in lymphocyte cultures. Boric acid did not show cytotoxic or genotoxic effects at the concentrations tested. Furthermore, the PAC-induced increases in the genotoxicity and cytotoxicity indices were diminished by the addition of BA. The present study suggests for the first time that BA can prevent the genotoxicity of PAC on human lymphocytes.

9

Biomonitoring of environmental pollution on the Algerian West coast using caged mussels Mytilus galloprovincialis

84%

Taleb Z. M., Benali I., Gherras H., Ykhlef-Allal A., Bachir-Bouiadjra B., Amiard J.-C., Boutiba Z.

Oceanologia

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2009

|

tom 51

|

nr 1

63-84

An active biomonitoring study was carried out on the Algerian west coast using wild reference mussels (Mytilus galloprovincialis) sampled from the Kristel (K) site and transplanted in net cages during one month (between May and June 2007) to Oran Harbour (OH) and Mostaganem Harbour (MH), areas characterised by high levels of urban and industrial pollution. The biological response of the mussels was evaluated by their condition index and the use of a general stress biomarker (evaluation of lysosomal membrane stability: the neutral red retention time (NRRT) method), a genotoxic effects biomarker (determination of micronuclei (MN) frequency) and a neurotoxic effects biomarker (determination of the acetylcholinesterase (AChE) concentration). Compared to the K reference specimens, OH and MH caged mussels presented a significant decrease of NRRT in lysosomal haemocytes (56.45±26.48 min and 67.25±22.77 min, respectively) (78±16.97 min for K mussels), an MN frequency respectively 7.3 and 9 times higher in the haemocytes and the gill cells of the OH caged mussels, and 7.2 and 6.4 times higher in the two tissues of the MH caged mussels. Significant inhibition of AChE activity was noted in the gills (16.93±3.1 nmol min−1 mg prot−1) and the digestive gland (7.69±1.79 nmol min−1 mg prot−1) of the OH mussels, but only in the gills (23.21±5.94 nmol min−1 mg prot−1) of the MH mussels, compared to the organs of the K control specimens (35.9±6.4 nmol min−1 mg prot−1 in the gills and 11.17±0.49 nmol min−1 mg prot−1 in the digestive gland). This study reflects the interest in such in situ biomonitoring assays and the utility of these biomarkers for assessing the effects of pollution in the Algerian coastal marine environment.

10

Frequency of micronuclei induced in the mouse erythropoietic system by new generation oxazaphosphorines

84%

Mazur L., Opydo-Chanek M., Niemeyer U.

Acta Biologica Cracoviensia. Series Zoologia

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2008

|

tom 50

11

Structure of micronuclei in the germ line cells of Pelophylax esculentus

84%

Chmielewska M., Rozenblut-Koscisty B., Serwa E., Ogielska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2016

|

tom 58

|

nr 1

12

A simple method for production of amicronucleate cell lines in ciliates by cis.-dichlorodiammineplatinum II

84%

Dutta D., Sapra G. R.

Acta Protozoologica

|

1989

|

tom 28

|

nr 2

13

An European population of Bryometopus hawaiiensis Foissner, 1994 [Protozoa: Ciliophora]

84%

Olmo J. L., Tellez C.

Acta Protozoologica

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1996

|

tom 35

|

nr 4

14

Apoptosis and clonogenic survival in three tumour cell lines exposed to gamma rays or chemical genotoxic agents

84%

Kumala S., Niemiec P., Widel M., Hancock R., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

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2003

|

tom 08

|

nr 3

We compared the extent to which apoptosis is induced and clonogenicity reduced in three tumour cell lines - the human melanoma Me45 and promyelocytic leukaemia HL-60, and the rat rhabdomyosarcoma R1 - after exposure to the anticancer drugs etoposide and cis-platinum or to gamma radiation; each induces different types of DNA damage. Cells which readily underwent apoptosis did not necessarily show a correlated loss of clonogenicity; for example, Me45 cells showed the highest sensitivity to all three agents in clonogenic assays but much lower levels of apoptotic cells than R1 or HL-60 cells. These results show that the efficiency of the eradication of clonogenic cells by genotoxic agents does not solely depend on the induction of apoptotic processes, and suggest that the induction of apoptosis and suppression of clonogenicity are independent processes.

15

Nuclear morphology, polyploidy, and chromatin elimination in tissue culture of Allium fistulosum L.

84%

Joachimiak A., Ilnicki T.

Acta Societatis Botanicorum Poloniae

|

2003

|

tom 72

|

nr 1

The morphology of cell nuclei in callus obtained from root-tip meristems of Allium fistulosum L. (Monocotyledoneae, Alliaceae) was analysed. The most interesting phenomena observed in long-term callus culture were the different mechanisms of cell polyploidization, enlargement of telomeric segments of heterochromatin, and extensive chromatin elimination, associated with instability of nuclei size and DNA content. Protruding heterochromatin "spikes" were observed on the surface of some di- and polyploid nuclei. The presence of these spikes was connected with the formation of small heterochromatic micronuclei frequently found in the cytoplasm. It is suggested that these micronuclei are produced by direct elimination of heterochromatin from the interphase nuclei. Polyploid cells accumulated with each successive cell collection. The ploidy level attained by highly polyploid cells was 15C-220C. The shape of the nuclei and heterochromatin distribution suggest that polyploid nuclei in A. fistulosum tissue culture are produced by endoreduplication and by restitution cycles.

16

Genetics of symbiotic interactions between Paramecium bursaria and the intranuclear bacterium Holospora acuminata, natural genetic variability by infectivity and susceptibility

84%

Rautian M. S., Skoblo I. I., Lebedeva N. A., Ossipov D. V.

Acta Protozoologica

|

1993

|

tom 32

|

nr 3

17

Thiamine prevents X-ray induction of genetic changes in human lymphocytes in vitro

84%

Konopacka M., Rogolinski J.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 3

The effects of thiamine (vitamin B1) on the level of spontaneous or radiation-induced genetic changes in human lymphocytes in vitro were studied. Cultured lymphocytes were exposed to increasing concentrations of thiamine (0-500 Ug/ml) and irradiated with X-rays. The DNA damage was estimated as the frequency of micronuclei and apoptotic or necrotic morphological changes in fixed cells. The results show that thiamine alone did not induce genetic changes. A significant decrease in the fraction of apoptotic and necrotic cells was observed in lymphocytes irradiated in the presence of vitamin B1 at concentrations between 1-100 ug/ml compared to those irradiated in the absence of thiamine. Vitamin B1 at 1 and 10 Ug/ml decreased also the extent of radiation-induced formation of micronuclei. Vitamin B1 had no effect on radiation-induced cytotoxicity as measured by nuclear division index. The results indicate that vitamin B1 protects human cells from radiation-induced genetic changes.

18

The level of DNA damage and the frequency of micronuclei in haemolymph of freshwater messels Anodonta woodiana exposed to benzo[a]pyrene

84%

Woznicki P., Lewandowska R., Brzuzan P., Ziomek E., Bardega R.

Acta Toxicologica

|

2004

|

tom 12

|

nr 1

19

Application of the cytokinesis-block technique to genotoxicity studies L929 and CHO cells

84%

Ejchart A.

Folia Histochemica et Cytobiologica. Supplement

|

1997

|

tom 35

|

nr 2

20

Clastogenic effects of antioxidant ethoxyquin observed in Vicia faba L. ssp. minor root meristem cells

84%

Kontek R., Osiecka R., Matlawska-Wasowska K., Blaszczyk A.

Acta Toxicologica

|

2006

|

tom 14

|

nr 1-2

Ograniczanie wyników

Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc

Genotoxic and haematological effect of commonly used fungicide on fish Clarias batrachus

Estimation of cytogenetic risk among coke oven workers exposed to polycyclic aromatic hydrocarbons

Induction of micronuclei in rat bone marrow after subchronic inhalation exposure to mixture of benzene, cyclohexanone and cyclohexane

Frequency of micronuclei in mouse peripheral blood erythrocytes following WR-2721 application and X-irradiation

Ultrastructural peculiarities of the meiotic prophase in the ciliate Tracheloraphis totevi [Karyorelictida]: synaptonemal complexes and sheaths around bivalents

Cytoplasmic zones responsible for differentiation of the new macronucleus and degradation of the old in Chilodonella steini (Trithigmostoma steini)

Boric acid as a protector against paclitaxel genotoxicity