Wyniki wyszukiwania

1

Purification of rat liver arylsulfatase A and its microheterogenity assayed by crossed affinity-immunoelectrophoresis

100%

Wojczyk B., Hoja D., Litynska A.

Acta Biochimica Polonica

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1992

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tom 39

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nr 4

Arylsulfatase A (arylsulfatase sulfohydrolase) EC 3.1.6*1 was purified from rat liver by a procedure consisting of differential centrifugation, Con A-Sepharose and Blue Sepharose chromatography, PBE 94 chromatofocusing, DEAE-cellulose and gel filtration chromatography followed by preparative electrophoresis. A molecular mass of 132000 was estimated by gradient PAGE. Particular proteins were detected by Immunoelectrophoresis. Isoelectric focusing combined with Immunoelectrophoresis gave two peaks of arylsulfatase A» with isoelectric points of pH 3.9 and 4.5. Microheterogeneity of rat liver arylsulfatase A was studied by affinity immunoelectrophoresis with 9 different lectins. The presence of concanavalin A-, Lens cutinaris agglutinin-, Lotus tetragonolobus agglutinin- and wheat germ aggtutinin- reactive forms permitted assessment of the types of carbohydrate moieties in arylsulfatase A.

2

Distribution of apolipoprotein Al-containing lipoprotein subclasses in plasma of normolipidemic subjects

84%

Atmeh R. F., Kasasbeh A. Z., Abu Odeh M. R.

Acta Biochimica Polonica

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2010

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tom 57

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nr 3

The distribution of apoA-I among apoA-I-containing lipoprotein (AI-Lp) subclasses in plasma was studied by immunoblotting utilizing agarose gel matrix incorporating anti-apoA-I as the transfer medium. Nine AI-Lp subclasses were detected in the plasma of normolipidemics, with relative molecular masses ranging from 70 000 to ≥ 354 000 and diameters from 7.12 to ≥ 11.6 nm. The mass distribution of AI-Lp subclasses was significantly different between males and females, and some subclasses increased gradually with age while others decreased. There was a significant strong positive correlation between subclass 1 (Mr 70 000-75 000) and subclass 3 (Mr 105 000-126 000) in all subjects and age groups. Analysis of similar AI-Lp or HDL subclasses reported in the literature showed variability in the sizes reported by various workers. This stresses the need for a unified classification of such subclasses, and this work contributes to this direction. The quantitative nature of the method used in this work compared with the semiquantitative approaches used earlier makes it a better method for the study of the quantitative changes of the subclasses in various physiological and pathological states. The method helps to generate ideas for in vitro and in vivo studies of apoA-I exchange among subclasses and in vivo kinetic studies. Conclusion. Plasma level of the AI-Lp subclasses varied quantitatively with age and gender, and strong correlations were detected between some subclasses. This work contributes to a better classification of AI-Lp subclasses according to their size. Comparison of the method used here with the methods reported in the literature revealed its advantages.

3

Controlled cholesterol efflux from the aortic smooth muscle cells triggers microheterogeneity of plasma membrane lipids and induces modification of the mitochondrial topology

67%

Tukaj C., Syta-Ksiazek E., Zauszkiewicz A., Drozd K., Figarski A., Spodnik J. H., Wozniak M.

Folia Morphologica

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2009

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tom 68

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nr 4

244-246

It is generally accepted that phospholipids of plasma membrane display lateral segregation into small microdomains commonly known as lipid rafts. Such lateral lipid organization is under the control of cholesterol. Cholesterol depletion evolved by methyl-β-cyclodextrin (MCD) has been found to induce further marked perturbation in lateral lipid organization, evidenced in the high field part of electron paramagnetic resonance spectra of plasma membranes labelled with a spectroscopic probe, namely 5-doxyl-stearic acid (5DOXS). Such perturbation of surface lipid topo-logy has been found to induce distinct changes in the mitochondrial morpho-logy, i.e. switch from filamentous form into small granular form. (Folia Morphol 2009; 68, 4: 244–246)

4

Polymorphism of gonadotropin action; molecular mechanisms and clinical implications

67%

Huhtaniemi I. T.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 3

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Acta Neurobiologiae Experimentalis

1 Folia Morphologica

1 Abu Odeh M. R.

1 Atmeh R. F.

1 Drozd K.

1 Figarski A.

1 Hoja D.

1 Huhtaniemi I. T.

1 Kasasbeh A. Z.

1 Litynska A.

1 Spodnik J. H.

1 Syta-Ksiazek E.

1 Tukaj C.

1 Wojczyk B.

1 Wozniak M.

1 Zauszkiewicz A.

1 2010

1 2009

1 1996

1 1992

Purification of rat liver arylsulfatase A and its microheterogenity assayed by crossed affinity-immunoelectrophoresis

Distribution of apolipoprotein Al-containing lipoprotein subclasses in plasma of normolipidemic subjects

Controlled cholesterol efflux from the aortic smooth muscle cells triggers microheterogeneity of plasma membrane lipids and induces modification of the mitochondrial topology

Polymorphism of gonadotropin action; molecular mechanisms and clinical implications