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The aim of this work was to evaluate in vitro the effect of replacing 0%, 50%, 75% or 100% of cereal-based concentrate in diets based on lucerne hay with feed blocks containing barley grain or 650 g · kg–1 fresh matter of greenhouse waste fruits (tomato, cucumber, or a 1:1 mixture of tomato and cucumber) on ruminal fermentation, methane production, and bacterial and methanogen population sizes. The type of feed-block showed no effect (p ≥ 0.25). The level of concentrate replacement with blocks did, however, affect (p ≤ 0.042) the pH, CH4 concentration, organic matter degradation rate, total gas, CH4 and total VFA production, acetate/propionate and CH4 /total VFAs ratios, and molar proportions of acetate and butyrate, without changing (p ≤ 0.082) methanogen and total bacteria abundance. Increasing levels of concentrate replacement with feed blocks modified ruminal fermentation, dry matter and neutral detergent fibre digestibility, and had an antimethanogenic effect.
The effects of genetically modified (GM) soybean meal (SBM) and maize on the diversity and activity of microbiota inhabiting terminal gut segments in broiler chickens Were studied. Eight diets were prepared, based on conventional or GM SBM combined with maize cvs Clarica or PR39 F58, or their isogenic MON 810 counterparts cvs Bacilla or PR39 F56. Diets were fed from age 1 to 28 days to 144 Ross broilers, allocated to eight groups of 18 birds each. The microbiota was analysed by terminal restriction fragment length polymorphism (T-RFLP) analysis and its activity was measured. In the ileum and caecum of all groups, members representing the orders Clostridiales, Lactobacillales and Selenomonadales were present, accompanied by Bifidobacteriales in the caecum. The diversity of the order Lactobacillales in the ileum and caecum of birds fed GM maize was reduced, while that of Lactobacillales in the ileum and Bifidobacteriales in the caecum of birds fed GM SBM was higher compared with conventional maize and SBM. The use of GM and conventional maize and SBM did not affect the activity of microbiota measured as bacterial enzyme activity and the concentration of short-chain fatty acids in the ileal and caecal digesta. The GM maize did not change resistance of E.
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Metabolic effects of dietary apple seed oil in rats

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The aim of this study was to determine the fatty acid profile of apple seed oil and its effects on the caecal functions, blood lipids, and markers of antioxidant status and inflammation in rats. A nutritional experiment was performed on Wistar rats allocated to 3 groups of 8 animals each. The animals were fed with a diet containing different sources of fat: pork lard (group LA), rapeseed oil (group RO) and apple seed oil (group AO). Apple seed oil was rich in linoleic acid and oleic acids (57 % and 32.3 % of total fatty acids, respectively). The short chain fatty acid concentration in the caecal digesta was comparable among all groups, whereas the ammonia concentration was lower in groups AO and RO than in group LA (0.32 and 0.3, respectively vs 0.42 mg/g). The plasma alanine (ALT) and aspartate transaminase (AST) activities also decreased in the AO and RO groups (ALT, 19.34 and 19.81, respectively vs 30,7 U/L and AST, 115.1 and 107, respectively vs 138.3 U/L) The plasma triacylglycerols (TG) concentration and the atherogenic index (ATI) of plasma were significantly decreased in the AO group compared to the LA group (TG, 1.79 vs 2.62 mmol/L and ATI, 0.095 vs 0.313). Apple seed oil is a valuable source of unsaturated fatty acids and its dietary addition has slightly better metabolic effects on rat organism than does rapeseed oil.
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Background. Candida is a strain of fungi that is found on the surface of healthy mucous membranes of the oral cavity. Yet, Candida may also be considered opportunistic microorganisms. The pathogenicity of Candida spp. depends on strain-specific characteristics including, invasiveness, adhesion, and production of biofilm abilities as well as the production of enzymes allowing the colonization of tissue. The aim of the paper was to evaluate the frequency of Candida spp. occurrence in the oral microbiota of healthy adults, and its relation with risk factors and colonization. Material and methods. This study consisted of 72 healthy participants (from the Lubelskie Voivodeship between 19 and 73 years of age). Medical history was collected to determine potential relationships to risk factors affecting oral microbiota (past oral cavity diseases, chronic diseases, and smoking). The participants who were qualified had no oral cavity infections during the study. The collected clinical samples (oral cavity swabs) were disseminated on the chromogenic base for isolation and differentiation of Candida spp. Results. The frequency of Candida spp. occurrence was at the level of 63.8%. C. albicans was the most frequently identified species (56.5%). C. glabrata (45.6%) and C. tropicalis (23.9%) were identified less often. In 21.7% of swabs, two species of Candida were identified and in 2.2% of swabs three species identified. The most common colonization risk factor was associated with smoking (32.6%). Conclusions. Yeasts of Candida species are part of the healthy microflora of the oral cavity in people of different ages and may occur as single species or coexist with other species. The coexistence of chronic diseases, propensity for oral cavity infections and smoking significantly influence the Candida colonization. This may result in future clinical consequences, for example in cases of immunodepression.
The study of biological properties of filmforming Staphylococcus aureus strains, including its sensitivity to bacteriophages, is one of the important tasks of laboratory diagnostic service that allows, if it necessary, choose an alternative treatment strategy and to establish the sources of strains. In the study of material from the nose and throat of 26 persons-volunteers the strains of S. aureus were selected. Among them 16 (61.5%) were able to form biofilms. Analysis of resistance to phages drugs of filmformation strains of S. aureus showed that 87.5% strains were sensitive the phages drugs "Piobacteriophage" and "Bacteriophage staphylococcal liquid". Phage type was identified for 9 (56.3%) strains. 64.3% of these strains were sensitive to the 1 phage from set, 21.4% – to 2 and 14.3% – 3 phages. It was determined that 77.8% strains were typed by phages from third group. For 1 strain detected simultaneous sensitivity to phages of 2 and 3 groups. With phage 81 (out of group phage) interacted 4 strains: 2 showed sensitivity only to him and another 2 were also susceptible to phage of third group.
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