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The aim of this work was an assessment of a microbiological quality of some dried herbs, offered in retail. A basil (Ocimum basilicum L.), marjoram (Origanum majorana L.), oregano (Origanum vulgare L.) savory (Satureja hortensis L.), tarragon (Artemisia dracunculus L.) and thyme (Thymus vulgaris L.) were investigated. The research material included 28 samples coming from five producers and was purchased in Szczecin in shops of various commercial networks. It was stated that the microbiological contamination was significantly differentiated depending on both: a kind of herbs and a producer. A mean count of microorganisms was the highest in basil while the lowest counts were detected in oregano and savory. A microbiological quality of the majority of the tested herbs attained standards. However, in 25% of the samples an excessive amount of bacteria (>105cfu∙g-1) was detected. Only in individual cases, there was a high contamination by moulds (>103cfu∙g-1) and coliforms (present in 0.01–0.001 g). Nevertheless, there were no E. coli, Salmonella sp. and coagulase (+) staphylococci in any sample.
Background. Pullulan is a water-soluble polysaccharide produced by fungi Aureobasidium pullulans. This glucan was applied for coating of food products. The aim of this study was obtaining of a pullulan coating and checking its effect on growth of microorganisms responsible for spoilage of food. Materials and methods. Pullulan produced by the white mutant A. pullulans B-1 was applied. Permeability of oxygen and carbon dioxide through film produced from 10% water solution of pullulan was checked as well as degree of inhibition of chosen microorganisms through pullulan coating formed on surface of growth‟s media. Results. Low permeability of gases through pullulan film and a considerable growth‟s limitation of all tested microorganisms were found. A total growth‟s inhibition of 21 from 36 tested strains and a partial growth‟s limitation of the remaining 15 strains was observed. The inhibitory effect was diverse and it was from 63 to 100%. Conclusions. These results proved that pullulan coating applied in these tests revealed big barrier characteristics in relation to oxygen and carbon dioxide, which had effect upon growth‟s inhibition of most of the tested microorganisms, responsible for spoilage of food
Research was conducted for the present paper on microbiological contamination of the air within the City of Toruń. It follows from the studies conducted that microorganisms occurred in greater numbers in the air within the area of the Old Town than in the Rubinkowo residential district. The number of microorganisms in the air was subject to seasonal changes. The highest numbers of heterotrophic mesophylic bacteria (CFU 37°C) were found in the summer season and the lowest in the winter season. The majority of isolated bacteria belonged to Gram positive forms - bacilli or cocci. A significant proportion of the isolated bacteria displayed antibiotic resistance to commonly used antibiotics.
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The aim of this study was bacteriological assessment of the dental unit waterlines (DUWL) biofilm - concentration and composition of the aerobe and facultative anaerobe bacterial microflora, and evaluation of the influence of a disinfecting product, Oxygenal 6, on the biofilm composition. Tubing fragments were taken from 25 units twice, before and after disinfection, and bacterial suspension of the biofilm was obtained from the samples. The bacterial flora was determined with the plate culture method. Bacteria were identified with biochemical microtests: API 20E, API 20NE (bioMerieux, France) and GP2 MicroPlateTM (BIOLOG, USA). Before disinfection, the following bacteria were identified: Gram-negative bacteria - Ralstonia pickettii, Pseudomonas vesicularis, Sphingomonas paucimobilis, Xanthomonas maltophilia; Gram-positive cocci - Micrococcus luteus, Micrococcus lylae, Staphylococcus cohnii, Staphylococcus lentus, Staphylococcus spp., Streptococcus spp.; Actinomycetes - Streptomyces albus. The prevailing bacteria were: Ralstonia pickettii (78.62%), found in all the units, and Sphingomonas paucimobilis (20.45%). After DUWL disinfection, Sphingomonas paucimobilis (88.79%) dominated in the biofilm, Staphylococcus spp. - 5.61% and Pseudomonas spp. - 3.74% were next most frequently occurring bacteria, and in more than a half of the biofilm samples 100% reduction of the bacterial microflora occurred. This study confirms effectiveness of Oxygenal 6 in bacterial decontamination of the DUWL biofilm.
Contamination with bioaerosols most affects the livestock production, and especially the poultry. Prolonged or repeated exposure to high concentrations of airborne fungal spores is considered a major risk factor for human health and contributes to the deterioration of lung functions, and particularly allergic diseases. The aim of the study was to determine the number and quality of moulds and yeasts in the air of various objects for poultry post-slaughter waste processing. The study allowed to detect the presence of moulds and yeasts in the air of all sampling points. There were: Aspergillus sp., Penicillium sp., Scopulariopsis sp., Trichoderma sp., Acremonium sp., Cephalosporium sp., Alternaria sp., Pithomyces sp., Eurotium sp., and: Rhodotorula sp., Candida sp., Yarrovia sp. and Saccharomyces sp. Statistical analysis confirmed the presence of highly significant influence of the sampling point, date, and interaction of these factors on the fungi population.
The potential role of currency in the spread of pathogenic microflora has been evaluated in many countries. In this study Polish paper notes and the coins in general circulation were assayed for the presence of cultivable bacteria and fungi. Bacterial isolates identification was based on cultural and biochemical characters and by comparison of the 16S rRNA gene sequence. Fungal isolates were recognized with biochemical and morphological criteria. Coagulase-negative staphylococci, (43.6% of the total bacterial count) including Staphylococcus saprophyticus, S. epidermidis, and S. hominis, and Enteroccus spp. (30.8% of the total bacterial count), i.e. E. faecalis, E. faecium and E. durans, were the most numerous bacterial contamination. Penicillium spp., and Aspergillus spp. were the most frequently detected moulds whereas Candida spp. was the most frequent yeast isolated from currency. A visible dependence between the banknote denomination, the physical condition of paper currency, and the number of bacteria and fungi was found. The overall count of bacteria isolated from currency was thousand-fold higher than that of fungal isolates. The total amount of bacteria and fungi recovered from the coins was approximately 2.7-fold lower than that isolated from the notes. In summary, the Polish currency notes were found to be contaminated mainly with commensal bacteria and fungi while the opportunistic pathogenic microorganisms Escherichia coli, Pseudomonas stutzeri and C. albicans were detected at a low frequency.
Background. Bacterial contamination is a major problem for commercial fuel ethanol production in distilleries all over the world. The contaminating microorganisms produce acetic and lactic acid that has a detrimental effect on fermentation efficiency. The aim of this work was to calculate the number of bacterial contaminants in some distilleries. Moreover, in this study it was signified what kind of bacteria contaminate ethanol production process. Material and methods. Grains were obtained from five distilleries from some regions in Poland, in this work hereafter referred to as α, β, γ, δ, and ε distilleries. Corn was the raw material in the α, β, and γ distilleries, triticale in δ distillery, and rye in the ε one. From these five distilleries, sweet mashes during fermentation and after it, were also analysed. The total number of microorganisms, the number of lactic acid bacteria, the number of anaerobic bacteria and the quantity of yeasts and moulds in raw materials were calculated. Results. The number of total viable bacteria (CFU/g), lactic acid bacteria (CFU/g), anaerobic bacteria (CFU/g), moulds, and yeasts (CFU/g) occur in the samples were determined. In all distilleries tested, all groups of microorganism were present. Conclusions. The results of our study show that all tested distilleries have a lot of difficulties with microbiology pollution which leads to a decrease of ethanol production and economical problems. From the economical point of view, reduction of microbial contamination makes it possible to increase the production volume
The aim of the study was to determine the level of air bacteria and fungi in the horse stable and the emission of microorganisms to the external environment during different seasons of the year. The predominating fungi in the stable and outside were also identified. These results demonstrate that both in the stables and outdoors, the number of microorganisms was the highest in the summer. This tendency was especially noticeable in the case of the concentrations of fungi outside the building, when the average value was significantly higher (P<0.01) than in other seasons. No statistically significant differences in the concentrations of airborne microorganisms at different distances from the stable were observed. Penicillium sp., Fusarium sp., and yeast predominated inside and outside the stable.
Background. Microbiological purity of cosmetics provides safety of users during their use, prevents physicochemical changes of a preparation, infections and diseases of the skin. Objective. The aim of this study was to assess the level of microbiological contamination of cosmetics used by one person and by several people and cosmetics after their expiry date in relations to standards for marketed cosmetics, ensuring safety of their use. Material and Methods. This study was conducted using 55 samples representing 19 types of cosmetics, divided into three groups: used by one person, used by several people and after the expiry date. In cosmetic samples the general numbers of aerobic mesophilic bacteria were determined with the spread plate method on tryptic-soy agar. The presence of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans were also checked. Results. The number of aerobic mesophylic bacteria in the tested cosmetics ranged from the level below the method detectability to 1.3×107 cfu/g or ml. The presence of Staphylococcus spp. was found in 11 (20.0%) tested cosmetic samples and of P. aeruginosa in one tested preparation. Yeasts C. albicans were not detected, whereas contamination with fungi Aspergillus spp. and Penicillium spp. ranging from 0.5×101 to 1.5×101 cfu/g or ml was recorded in four cosmetics. The level of microbiological contamination of cosmetics used by several people was higher than that of cosmetics used by one person. Cosmetics after the expiry date showed the highest microbiological contamination. Conclusions. The number of users of cosmetic and it expiry date exceeding influenced the level of microbial contamination of preparations.
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Mikologiczne skazenia zywnosci

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W artykule opisano zanieczyszczenie żywności pleśniami i omówiono warunki sprzyjające wytwarzaniu mikotoksyn. Wzrost pleśni powoduje pogorszenie jakości lub zepsucie produktów spożywczych, co może prowadzić do zmiany wyglądu, nieprzyjemnego zapachu, obniżonej zdolności kiełkowania nasion, skażenia mikotoksynami i/lub alergii i infekcji u ludzi. Wysoka toksyczność metabolitów pleśni jest dużym zagrożeniem dla konsumentów. Tworzenie mikotoksyn następuje wyłącznie w wyniku wzrostu pleśni toksynotwórczych. Szczególny wpływ na rodzaj występujących pleśni ma skład żywności. Natomiast rozwój i wzrost pleśni w znaczącym stopniu uwarunkowane są: aktywnością wody (aw), temperaturą, wartością pH, zawartością O2 i CO2, rodzajem substratu i składem mikoflory.
Celem pracy było określenie poziomu zanieczyszczenia grzybami strzępkowymi suszonych ziół oraz przypraw dostępnych w sprzedaży detalicznej w sklepach ekologicznych i hipermarketach województwa łódzkiego. Badaniami objęto 13 rodzajów produktów: czosnek (Allium sativum), koper (Anethum graveolens), natkę pietruszki (Petroselinum crispum), bazylię (Ocimum basilicum), oregano (Origanum vulgare), estragon (Artemisia dracunculus), tymianek (Thymus vulgaris), majeranek (Origanum majorana), gałkę muszkatołową (Myristica fragans), curry (Helichrysum angustifolia), kurkumę (Curcuma longa), pieprz czarny (Piper nigrum), paprykę słodką (Capsicum annuum). Analiza mikrobiologiczna obejmowała oznaczenie liczby grzybów pleśniowych i ich skład jakościowy. Zanieczyszczenie badanych ziół oraz przypraw przez grzyby strzępkowe było zróżnicowane i nie przekraczało poziomu 10⁴ jtk·g⁻¹.W czterech produktach: bazylii, curry, kurkumie i papryce słodkiej próbki zakupione w hipermarkecie charakteryzowały się istotnie większym zanieczyszczeniem przez pleśnie w porównaniu z próbkami pochodzącymi ze sklepu ekologicznego. Wykazano brak zróżnicowania poziomu zanieczyszczenia grzybami strzępkowymi badanych produktów w zależności od miejsca zakupu. Średnia liczba pleśni w ziołach i przyprawach zakupionych w sklepach ekologicznych oraz w produktach pochodzących z hipermarketu kształtowała się na podobnym poziomie. Stwierdzono natomiast zróżnicowanie poziomu zanieczyszczenia pleśniami w zależności od rodzaju przyprawy. Wyizolowane z suszonych ziół oraz przypraw grzyby strzępkowe należały łącznie do 5 rodzajów. Zidentyfikowano pleśnie z rodzaju Aspergillus (A. niger, A. flavus), Penicillium, Mucor, Alternaria i Cladosporium.
Podjęto próbę uchwycenia zależności pomiędzy stopniem bakteriologicznego skażenia środowiska i jakościowym skladem potencjalnie chorobotwórczych grzybów keratynolitycznych w próbkach gleby, osadów dennych i powietrza z dzielnicy Gliwic - Łabędy.
High hydrostatic pressures (HHP) gain recently increasing interest as potential non-thermal alternative of food preservation. The purpose of the experiment was the evaluation of HHP efficiency against Salmonella enteritidis and Escherichia coli, contaminating the raw poultry meat. The studies were performed on poultry meat samples inoculated superficially with 107 CFU x g-1 of the test bacteria. Subsequently the samples were pressurized for 15 minutes (200, 300 or 400 MPa). Pressurization reflected in significant bacterial reduction. Histological analysis of treated meat samples revealed structural alterations of intramuscular connective tissue and loss of muscular stratification, proportional to the amount of pressure applied. Concluding, HHP seem to reduce efficiently the level of microbiological meat contamination and, probably, also increase the dietary availability of sarcoplasmic proteins.
Polskie normy z 1989 r. dotyczące badania skażenia mikrobiologicznego powietrza atmosferycznego w dużym stopniu uległy dezaktualizacji. Polskich norm (PN) dotyczących badania zanieczyszczeń biologicznych w pomieszczeniach wewnętrznych brak. Utrudnia to lub uniemożliwia porównanie uzyskiwanych wyników między sobą i z normami zagranicznymi.
W programach walki z zagrożeniami mikrobiologicznymi w tej dynamicznie rozwijającej się branży oczekuje się między innymi zmniejszenia liczby zatruć pokarmowych poprzez ograniczenie stopnia zanieczyszczenia powierzchni tuszek drobiowych przy pomocy metod fizycznych lub chemicznych. Obok stosowania pochodnych chloru (np. dwutlenku chloru) lub fosforanu trójsodowego w wodnym systemie mycia i chłodzenia tuszek wymienia się nadtlenek wodoru oraz kwasy nadtlenowe, np. kwas nadoctowy. W niniejszej pracy sprawdzono możliwości wykorzystania kwasu nadmrówkowego jako alternatywnej, innowacyjnej i przyjaznej środowisku chemicznej metody eliminacji wybranych zanieczyszczeń mikrobiologicznych z powierzchni tuszek drobiowych z uwzględnieniem wszystkich wymienionych powyżej aspektów. Na podstawie przeprowadzonych badań potwierdzono, że stężenie kwasu nadmrówkowego w badanym preparacie w ciągu 7 dni ulegało nieznacznej zmianie. Stan maksymalnego stężenia substancji czynnej osiąga się około 24 godzin po zmieszaniu składników preparatu. Zauważono brak istotnego wpływu badanego preparatu na obiektywne parametry barwy tuszki drobiowej po obróbce chemicznej. Przyjęto stężenie preparatu kwasu nadmrówkowego (110 ppm), co okazało się skuteczne wobec ogólnej liczny bakterii oraz bakterii z rodziny Enterobacteriacae, występujących na tuszce drobiowej.
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