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Field study was performed on Haplic Luvisol soil to determine the effects of soil compaction and straw mulching on microbial parameters of soil under soybean. Treatments with different compaction were established on unmulched and mulched with straw soil. The effect of soil compaction and straw mulching on the total bacteria number and activities of dehydrogenases, protease, alkaline and acid phosphatases was studied. The results of study indicated the decrease of enzymes activities in strongly compacted soil and their increase in medium compacted soil as compared to no-compacted treatment. Mulch application caused stimulation of the bacteria total number and enzymatic activity in the soil under all compaction levels. Compaction and mulch effects were significant for all analyzed microbial parameters (P<0.001).
The aim of this work was to test the suitability of microbiological methods for the ecotoxicological evaluation on the example of soils of two sites (I and II) (more and less contaminated and situated in Saxony-Anhalt, Germany). Contents of Zn, Pb, Cd, Ni, Cr, and Cu in soil ranged 787–210, 210–110, 8–1, 49–12, 130–17, and 161–70 mg kg⁻¹, respectively being significantly higher in site I. The sites differ also in respect to pH (5.2–7.5), Corg (5.66–8.27%) and type of soil. The parameters tested were following: substrate-induced respiration (SIR), activity of luminous bacteria, substrate utilization patterns on BIOLOG ECO-and GN-plates, and phospholipid fatty acid (PLFA) profiles. The respiratory coefficient QR, peak respiratory maximum and BIOLOG ECO-plates were suitable for an ecotoxicological assessment of contaminated soils. QR values (> 0.3) and peak maximum values (> 40 h) indicated stress of soil microorganisms at the most heavy metal contaminated site (site I). PLFA analysis can be used to detect various environmental stresses in the soil. The trans/cis ratio of monosaturated fatty acids (> 0.1) and the fungal/bacterial biomass ratio were able to distinguish the stress conditions in soils connected with heavy metal contamination. With increasing heavy metal content in soils the PLFA pattern are changed directionally.
Plant essential oils and their active components have shown a large scale of activities: as antimicrobials, anti-oxidants, digestive stimulators, anti-inflammators, appetisers and performance enhancers. The antimicrobial properties of the essential oil from sage (Salvia officinalis L., family Lamiaceae) were evaluated against selected bacteria in a model experiment in crossbred piglets (Slovak White x Pietrain) weaned at 10 days of age. The essential oil was applied daily in a dose of 0.05% into the commercial feed mixture ČOS 1 and ČOS 2 for a period of three weeks, starting at the age of 21 days to 7 piglets in an experimental group and pathogen concentrations were compared with a control group of 3 piglets. Faecal samples of piglets were analyzed on the 21st, 35th and 42nd days of age and counts of anaerobes, Escherichia coli, enterobacteria and enterococci were performed. The differences in counts of selected bacteria within the control group during the experiment were not statistically significant. The counts within the experimental group showed statistically significant differences (p<0.05) in number of all selected bacteria between 1st and 2nd samplings. The anaerobic bacteria count was also significantly different on 21st and 42nd day of age. Comparing the control and the experimental group counts of Escherichia coli at the age of 35 days were significantly lower in the experimental group. All statistically significant differences observed showed decrease in selected bacteria counts. No adverse effects on animal health were noticed when using sage essential oil. Therefore, sage oil may be recommended to be an alternative fytoaditive antimicrobial supplement to conventional additives used in animal feed. (Because of the essential oil is inhibitory to selected pathogenic microorganisms it may provide alternative and supplement to conventional antimicrobial additives in foods.)
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