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Non-lipophilic mycobiota of human skin

100%

Talaga K., Krzysciak P.

Acta Mycologica

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2015

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tom 50

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nr 2

The human skin is inhabited by many species of bacteria and fungi, which are its natural microbiota. Fungi colonizing the skin, including those causing disease, characterized by great variety and variability, can be influenced by various factors. The purpose of this study was to investigate the composition of the non-lipiddependent fungal microbiota of skin, including the presence of species potentially pathogenic for humans. Fifty-six volunteers of both sexes aged 22–78 were subjected to the study. Swabs were taken from the face, chest, back and interdigital spaces of hands. Mycobiota isolated proved to vary both in terms of the location of occurrence and gender of patients. Interdigital spaces of hands, dominated by yeasts, constitute a location on human skin most contaminated with fungi. Molds were more often isolated from the face and chest. The back was the least contaminated location. There was no difference in fungal incidence in relation to sex.

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Assessing the microbial diversity and proximate composition of smoked-fermented bushmeat from four different bushmeat samples

100%

Amponsah A. S., Ankar-Brewoo G. M., Lutterodt H. E., Ofosu I. W.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2024

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tom 105

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nr 1

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Life in extreme habitats: diversity of endolithic microorganisms from cold desert ecosystems of Eastern Pamir

84%

Khomutovska N., Jerzak M., Kostrzewska-Szlakowska I., Kwiatkowski J., Suska-Malawska M., Syczewski M., Jasser I.

Polish Journal of Ecology

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2017

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tom 65

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nr 4

The aim of this study was to identify cyanobacteria diversity in rock communities from the cold desert ecosystem in Eastern Pamir Mountains (Tajikistan) and assess if the rock type and rock`s porosity can be indicators of microbial diversity in this extreme environment. Seven samples were collected in July 2015 from hillsides (ca 4000–4500 m a.s.l.) of the Eastern Pamir Mountains. Petrographic and scanning microscopy (SEM) allowed for the characterization of the rocks inhabited by endolithic communities as granite, gneiss and limestone with variable porosity. Based on next-generation sequencing (NGS) of amplicon of V3–V4 hypervariable region of 16S rRNA gene, we established that Actinobacteria,Proteobacteria and Cyanobacteria dominated the endolithic communities of microorganisms in the rocks studied, which distinguishes these communities from those described for other cold arid regions. Chroococcidiopsis and Leptolyngbya were dominant genera in the cyanobacterial communities according to culture-dependent analysis, as well as microscopic analyses of endoliths scraps from the rocks. Culture-independent metagenomic analyses revealed that Microcoleus, Acaryochloris, Chroococcidiopsis and Thermosynechococcus reads were the most abundant from all reads and dominated interchangeably in the samples. Endolithic communities of microorganisms in the rocks from the cold desert shrubland of Eastern Pamir Mts. appear to be diverse and different from communities described for other cold deserts.

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Effects of emergent plant species and growth strategy on microbial community structure and diversity

84%

Wang A.-L.

Polish Journal of Environmental Studies

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2013

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tom 22

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nr 5

A field study was conducted in two adjacent shallow lakes (Aiwan Lake and Qingnian Lake) in Tianjin, China, to investigate the effects of plant species and growth strategy (single or mix) on the microbial community’s structure and diversity in the rhizosphere of emergent plants by phospholipid fatty acid (PLFA) methods. The results demonstrated that microbial biomass was higher in the Typha orientalis (T. orientalis) rhizosphere than that in the Phragmites australis (P. australis) rhizosphere, whether they grew separately or together. The bacterial population of gram-positive bacteria (G⁺) was found to be less than that of the gramnegative bacteria (G⁻) in all samples, and the ratio of G⁺ to G⁻ in the plant rhizosphere was less than that in the non-rhizosphere. The diversity index of plant rhizosphere was higher than that of the non-rhizosphere, and was higher in the T. orientalis rhizosphere than in the P. australis rhizosphere. Cluster analysis demonstrated that microbial community structure was more significantly influenced by plant species than by growth strategy.

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Relationship between soil microbial diversity and bioremediation process at an oil refinery

84%

Plaza G., Ulfig K., Brigmon R. L.

Acta Microbiologica Polonica

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2003

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tom 52

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nr 2

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Biodiversity of freshwater microorganisms - achievements, problems, and perspectives

84%

Weisse T.

Polish Journal of Ecology

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2006

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tom 54

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nr 4

The extent and significance of the diversity of freshwater microbes is at present controversially debated. Until 1980 it was assumed that there are no freshwater-specific bacteria and that the total number of bacterial species is low. The advent of molecular tools over the last ten years revealed that there is a bacterial freshwater assemblage which is phylogenetically different from soil and marine bacteria; secondly, it became obvious that the total number of cultured bacterial species (~5900) underestimates bacterial diversity by several orders of magnitude. The current debate centres on 1) how to define a bacterial species and 2) if there is a microbial biogeography. The latter relates to the issue of ubiquity and cosmopolitanism, which is controversially discussed primarily in relation to eukaryotic microorganisms, namely ciliates. Although solid evidence is scarce, many microbial ecologists assume, in accordance with Baas Becking’s famous 70-year old dictum – “everything is everywhere, the environment selects” – that freshwater microorganisms are easily dispersed and, therefore, potentially cosmopolitan. This review focuses on the often neglected second part of Baas Becking’s metaphor. Evidence is accumulating rapidly that the environment does not simply act as a filter sensu Gleason’s individualistic concept for widely dispersed microbes. Rather, prokaryotic and eukaryotic microorganisms have adapted to their specific habitat and perform better in this environment than newly invading congeners. There is an enormous ecophysiological diversity among closely related freshwater microbes which is neither obvious at the morphospecies level nor at the level of evolutionarily conserved genes, such as the small ribosomal RNA gene. Although this large diversity has been demonstrated for various groups of bacteria and protists, there is currently no measure available to compare microbial biodiversity across prokaryotic and eukaryotic domains. The current challenge is to link genetic divergence to ecophysiological diversity in the major taxa.

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Effect of fly ash application on some soil physical properties and microbial activities

67%

Kohli S. J., Goyal D.

Acta Agrophysica

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2010

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tom 16

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nr 2[182]

Electrostatic precipitator (ESP) fly ash obtained from a thermal power plant was mixed with a slightly alkaline soil on w/w basis for use as a potting mixture for poplar nursery and investigated for its influence on microbial activity and physical properties. Application of fly ash as an amendment @ 10% was found to be optimum for bacterial population, soil dehydrogenase activity and microbial biomass. Water holding capacity of fly ash amended soil increased and bulk density decreased as result of fly ash addition as compared to unamended soil. The suitability of fly ash to be used as a soil ameliorant in nursery plantations attains significance from the point of view of eco-friendly disposal of fly ash.

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Problematic biases in the availability of molecular markers in protists: the example of the dinoflagellates

67%

Gomez F.

Acta Protozoologica

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2014

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tom 53

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nr 1 Spec.is. Marine Heterotrophic Protists

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The impact of organic carbon and ammonia load in wastewater on ammonia-oxidizing bacteria community in activated sludge

67%

Cydzik-Kwiatkowska A., Wojnowska-Baryla I.

Polish Journal of Microbiology

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2008

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tom 57

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nr 3

The experiment was carried out in two SBR reactors differing in ammonia load (reactor R1 - ammonia load of 130 mg N-NH₄ x d⁻¹, reactor R2 - ammonia load of 250 mg N-NH₄ x d⁻¹). Feeding conditions in the reactors were switched from favoring autotrophic nitrification through favoring heterotrophic processes and back to autotrophic conditions. Observations of ammonia-oxidizing bacteria (AOB) community changes were based on PCR-RFLP analysis of amplified amoA gene fragments and AOB genetic diversity was evaluated on the base of the number of different amoA gene forms. When only carbonates were introduced with wastewater restriction patterns established about day 23 and 28 at ammonia load of 250 and 130 mg N-NH₄ x d⁻¹, respectively. In both reactors statistically higher number of different amoA gene forms was observed when only carbonates were present in wastewater in comparison to conditions in which sodium acetate was introduced to the reactors. The AOB participation in activated sludge was higher at ammonia load of 250 mg N-NH₄ x d⁻¹ but their genetic diversity was lower in comparison with this observed at ammonia load of 130 mg N-NH₄ x d⁻¹.

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