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The aim of this study was to determine the in vitro resistance of aerobic bacteria isolated from the uterine pathological secretion of 312 dairy cows with clinical metritis and clinical endometritis to antibiotics. Animals with pathological discharges from the vagina observable between the 7th and 50th day after parturition were diagnosed clinically per vaginam and per rectum and then swabs from uteri lumen were aseptically collected. Bacteriological examinations of swabs were performed according to commonly accepted rules. Sensitivity to antibiotics was tested by the disk diffusion method and performed according to CLSI (formerly NCCLS) guidelines in Mueller-Hinton agar. The bacteria isolated were mostly Arcanobacterium pyogenes, Escherichia coli, non E. coli Gram-negative rods, Streptococcus species and Staphylococcus species. Strains of Arc. pyogenes were the most susceptible to amoxicillin/clavulanic acid (97.3%), ceftiofur (98%) and bacitracine (96.7%). E. coli isolates were the most susceptible to norfloxacin (100%), marbofloxacin (100%), rifaximin (97%), gentamycin (96%) and amoxicillin/clavulanic acid (95.5%). Other Gram-negative bacteria were the most sensitive to norfloxacin (100%), neomycin (100%) and cefoperazon (95%). Streptococcus species were the most susceptible to amoxicillin/clavulanic acid (94.6%), ampicillin (92.3%), norfloxacin (92%), cephapirine (88%), cefoperazone (86.5%), rifaximine (85.7%) and penicillin (84.9%). The highest in vitro activity against Staphylococcus spp. was demonstrated by amoxicillin/clavulanic acid (100%), norfloxacin (100%), neomycin (93.6%) and cefoperazone (85.7%). Arc. pyogenes were the most resistant to oxytetracycline and cloxacillin, E. coli to ampicillin and cephapirin, non-E. coli Gram-negative rods to ampicillin and cephapirin, Streptococcus spp. to neomycin and oxytetracycline, and Staphylococcus spp. to ampicillin.
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Predisposing factors for puerperal metritis in cows

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The investigation was performed on 90 multiparous cows in four farms. Animals were almost identically fed and had similar milk yield during last year (6000 kg milk). Blood samples were taken 3 times (-3, -2, -1 week) before and 2 times (+1, +3 week) after parturition. Some biochemical parameters were determined. In all farms the increase of free fatty acids level was observed after parturition. The decrease in cholesterol concentration and triglyceride level after calving were accompanied. Differences in valucs of analysed parameters (AST, FFA, glucose) in cows with correct and disturbed post partum period were observed before parturition.
The objeclive of this study was to determine whether changes of biochemical indicators and T lymphocyte subpopulations during the prepartum period would differ between primiparous dairy cows that developed clinical metritis after calving and those that remained healthy. Cows that developed clinical metritis (53.3%) showed significant differences in age of the first calving and yield of milk during the first month of lactation. Clinical metritis in primiparous dairy cows was not connected with metabolic changes before parturition. Lower percentage of CD2+, CD4+, and CD8+ in metritic cows may be linked to the immunosuppression observed in periparturient cows.
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