Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 11

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  methicillin
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Evolution and resistance expression of MRSA. Evaluation of beta-lactam antibiotics against a set of isogenic strains with different types of phenotypic expression

100%
Asada K., Inaba Y., Tateda-Suzuki E., Kuwahara-Arai K., Ito T., Hiramatsu K.
Acta Biochimica Polonica
|
1995
|
tom 42
|
nr 4
Methicillin-resistant Staphylococcus aureus (MRSA) has two mechanisms of resistance to P-lactam antibiotics; one is mediated by mecA gene expression, and the other by penicillinase production. It has been generally accepted in the clinical field that β-lactam antibiotics are not the drugs of choice for MRSA infection. In this report, however, ampicillin and penicillin G were shown to have relatively good activity against MRSA if combined with a β-lactamase inhibitor, sulbactam. These β-lactam antibiotics were found to have relatively high binding affinities to PBP2', the mecA-encoded MRSA-specific penicillin-binding protein. The possible therapeutic application of sulbactam/ampicillin against MRSA infection in combination with arbekacin, an aminoglycoside antibiotic newly developed and introduced into clinical use in Japan, is discussed.
2

Mechanism of vancomycin resistance in methicillin resistant Staphylococcus aureus

100%
Sieradzki K., Markiewicz Z.
Polish Journal of Microbiology
|
2004
|
tom 53
|
nr 4
A collection of laboratory mutants and clinical MRSA strains, additionally exhibiting resistance to glycopeptide antibiotics, was studied in detail. The nature of resistance to glycopeptides was found to be different from that existing in vancomycin resistant (VR) enterococci. The mutants produced abnormal murein in which the level of highly oligomeric muropeptides was drastically reduced. Biochemical and genetic analyses of Penicillin Binding Proteins (PBPs) showed inactivation of PBP4. Changes in other PBPs were not apparent, except for PBP2a that was inactivated in the highly VR mutant VM. Transposon inactivation of the pbpB gene and several other genes involved in synthesis of staphylococcal peptidoglycan all caused dramatic reduction of glycopeptide resistance in the staphylococcal mutants. While inactivation of PBP2a slightly increased the levels of glycopeptide resistance, a combination of vancomycin or teicoplanin with β-lactam inhibitors, chosen on the basis of their relatively selective affinities for individual staphylococcal PBPs completely inhibited the expression of glycopeptide resistance in MRSA. Glycopeptide antibiotics caused a virtually complete inhibition of cell wall turnover and autolysis and massive overgrowth of cell wall material in the glycopeptide resistant mutants. Bacteria were able to remove quantitatively glycopeptide molecules from the growth medium, and sequestered antibiotic could be recovered in biologically active form from the purified cell walls. These observations and the results of the vancomycin binding studies suggest alterations in the structural organization of the mutants' cell wall such that access of glycopeptide molecules to the sites of wall biosynthesis is blocked by steric hindrance.
3

Antibiotic resistance patterns and occurrence of mecA gene in Staphylococcus intermedius strains of canine origin

84%
Kizerwetter-Swida M., Chrobak D., Rzewuska M., Binek M.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 1
We have evaluated 102 Staphylococcus intermedius isolates of canine origin for susceptibility to antimicrobial primary agents, i.e. penicillin, amoxicillin, amoxicillin with clavulanic acid, cefuroxime, trimethoprim/sulfonamides, neomycin, streptomycin, gentamicin, norfloxacin, tetracycline, vancomycin, erythromycin and secondary agents, i.e. chloramphenicol, ciprofloxacin, lincomycin, teicoplanin, rifampicin, imipenem, mupirocin. Antimicrobial sensitivity was examined using the disk diffusion method and performed according to NCCLS quidelines. Methicillin resistance was detected using the disk diffusion method with oxacillin, and the occurrence of mecA gene was detected by PCR. Resistance to streptomycin, penicillin, amoxicillin, neomycin, followed by tetracycline was predominant. From 14 mecA-positive strains, 12 were multidrug-resitant, and the remaining two showed atypical susceptibility. One strain resistant to oxacillin in the disc diffusion method was mecA-negative, suggesting a different mechanism of resistance. Our results indicate that the emergence of S. intermedius resistance to methicillin may be underestimated. In case of clinical multidrug-resitant S. intermedius isolates, resistance to methicillin should be considered.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Heterogeneity of methicillin-sensitive Staphylococcus pseudintermedius strains isolated from diseased dogs

84%
Garbacz K., Piechowicz L., Zarnowska S., Haras K., Dabrowska-Szponar M.
Polish Journal of Veterinary Sciences
|
2011
|
tom 14
|
nr 2
Thirty nine canine S. pseudintermedius strains were examined for antibiotic susceptibility and genetic polymorphisms. All strains were methicillin-sensitive S. pseudintermedius (MSSP). Resistance to penicillin was most prevalent (66.6%), followed by resistance to neomycin (56.4%), erythromycin (53.8%), clindamycin (48.7%), chloramphenicol (48.7%), and tetracycline (46.2%). Pulsed-field electrophoresis (PFGE) showed a high genetic polymorphism in the investigated strains.
5

Staphylococcal cassette chromosome mec (SCCmec) classification and typing methods: an overview

84%
Turlej A., Hryniewicz W., Empel J.
Polish Journal of Microbiology
|
2011
|
tom 60
|
nr 2
Meticillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital-acquired infections, but since late 1990s also the community-acquired. For better understanding of the S. aureus epidemiology there is an urgent need for creation of new typing method for SCCmec element. The molecular typing of MRSA for epidemiological purposes is investigated by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), spa typing and the SCCmec type assignment. In last few years not only new type of SCCmec (VI to XI) have been identified, but also additional subtypes (i.e. IVg-j) and different variants of already existed one (i.e. 5C2&5 and 2B2&5) were discovered. The aim of this review is to briefly summarize current knowledge about SCCmec classification and to discuss advantages and disadvantages of selected SCCmec typing methods.
6

Phenotyping methods in epidemiological analysis of epidemic Staphylococcus aureus strains

84%
Konopka M., Trzcinski K., Tyski S., Hryniewicz W.
Acta Microbiologica Polonica
|
1996
|
tom 45
|
nr 3-4
7

Antibiotic susceptibility patterns of respiratory isolates of Staphylococcus aureus in a Turkish University Hospital

84%
Gonlugur U., Akkurt I., Ozdemir L., Bakici M. Z., Icagasioglu S., Gultekin F.
Acta Microbiologica Polonica
|
2003
|
tom 52
|
nr 2
8

New plant glycoprotein against methicillin resistant staphylococci and enterococci

84%
Fik E., Gozdzicka-Jozefiak A., Haertle T., Mirska I., Kedzia W.
Acta Microbiologica Polonica
|
1997
|
tom 46
|
nr 3
9

Antibiotic resistance of Staphylococcus aureus and coagulase-negative staphylococci isolated from bovine mastitis

67%
Turutoglu H., Ercelik S., Ozturk D.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 1
10

Comparison of simple methods of methicillin- resistance detection and evaluation of some properties of methicillin-resistant Staphylococcus aureus strains

67%
Trzcinski K., Zareba T., Tyski S., Hryniewicz W.
Acta Microbiologica Polonica
|
1993
|
tom 42
|
nr 1
11

Application of molecular methods for hospital infections control in Western Pomerania

67%
Giedrys-Kalemba S., Bilska I., Jursa J., Maczynska I., Czekajlo-Kolodziej U., Wojciechowska I., Szymaniak L.
Advances in Agricultural Sciences
|
2002
|
tom 08
|
nr 1
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.