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The antimicrobial activity of methanolic and aqueous extracts of Chrysophyllum albidum fruits was investigated against clinical isolates(Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Candida albicans). Qualitative phytochemistry of the plant indicated that the plant contained Flavonoids, Steroids, Alkaloids, Tannin, Anthraquinone and Cardiac glycosides while Saponins were reported absent. The maximum activity of the aqueous extracts in the test isolates was observed on Staphylococcus aureus, which showed clear zones with diameters of 24.0mm, 20.0mm and 16.5mm at concentrations of 100mg/ml, 50mg/ml and 25mg/ml respectively while it had low activity on Klebsiella pneumonia, with clear zones of inhibition of 15.0mm, 12.0mm and 10.5mm at same concentrations. On the other hand, Methanolic extracts activity on Staphylococcus aureus produced clear zones of 21.0mm, 17.5mm and 12.0mm at concentrations of 200mg/ml, 100mg/ml and 50mg/ml respectively as its best activity while the it had least observed activity on Klebsiella pneumonia with clear zones of 14.0mm, 11.5mm and 10.5mm at same concentrations. The aqueous extracts had greater activity than the methanolic extracts at same concentrations. Therefore, the fruit of the plant can be a good source of remedy in phytomedicine.
Methanolic extract of Elephantopus scaber leaves was investigated for its role in hair growth in experimental rats. The 2% and 5% solution of prepared formulation using methanolic extract was studied for hair growth on wistar rats for 30 days. Minoxidil (2% solution) was taken as a reference standard. The prepared hair oil formulation of methanolic extract significantly (p<0.01) potentiated the hair growth initiation and completion time with dosedependent improvement in skin biopsy, hair length and hair weight. In vitro hair follicles development study showed the significant (p<0.05) improvement in the initiation of new hair follicles. Above mentioned results indicated that the methanolic extract of E. scaber rendered significant hair growth promotive effect.
The aim of the study was to verify whether methanolic extracts prepared from plants of 26 selected species contained substances reducing feeding of Hylobius abietis beetles on shoots of Pinus sylvestris. Experiments were conduced under laboratory conditions. A statistically significant reduction in the size of damage was recorded on pine twigs treated with methanol extracts of leaves of Trifolium repens, Lupinus polyphyllus, Robinia pseudoacacia, Tanacetum vulgare, Nicotiana tabacum, Sambucus nigra, Convallaria majalis and Lycopersicon esculentum. Probably the strongest antifeedants were methanolic extracts of leaves of Lupinus polyphyllus, Robinia pseudoacacia and Convallaria majalis.
Toxicity and physiological alterations were determined in Pseudococcus viburni nymphs treated with Artemisia annua methanolic extract. The leaf dipping bioassay showed LC50 values of 0.287% and 0.194% 24 and 48 hours post-exposure. Activities of general esterases were significantly higher in the control nymphs than in those which had been treated except for the 48 h time interval using α-naphtyl acetate. The activity of glutathione S-transferase using CDNB (1-chloro-2,4-dinitrobenzene) in the control nymphs, was significantly higher than in the control at both time intervals while no significant difference was observed after 24 h in addition to the higher enzymatic activity in the treated nymphs after 48 h. All three aminotransferases were significantly more active in the control nymphs except for time intervals of 24 h for γ-glutamyl transferase and 48 h for alanine aminotransferase. Higher activities of lactate dehydrogenase, acid- and alkaline phosphatase were found in the control nymphs than in treated nymphs for all time intervals. Activities of the enzymes involved in the antioxidant system including catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase was increased in the treated nymphs compared to the control. Results of the current study demonstrated toxic effects of A. annua methanolic extract on P. viburni nymphs causing mortality and physiological turbulences.
The present study was quantitative analysis and antimicrobial activity of petroleum ether, chloroform, ethyl acetate, methanol root extracts. The gram positive and gram negative bacterial and fungal species by the disc diffusion method at various concentration (250μl-1000μl). The results was present in the phenolic and flavonoid content compared to extracts. All the root extract against to Bacillus subtilis and Streptococcus pyogenes and fungal species Candida albicans at highest inhibition at concentration of methanolic root extract of zone formation. The preliminary studies on Decalepis hamiltonii root extract exhibited their antimicrobial potential which could be exploited further and future pharmaceutical, therapeutic efficiency and food preservation and applications.
Antimycotic activity of Petroleum ether and 98% methanolic young leaf soxhlet extract of Solanum nigrum (Solanaceae) was evaluated against dermatophytic fungi namely, Trichophyton rubrum, Trichophyton tonsurans, Trichophyton mentagrophytes, Microsporium gypseum, Candida albicans, and bacteria like, Staphylococcus aureus, Psudomonas aeruginosa, Bacillus subtilis, Escherichia coli. The maximum activity was observed in interpolar methanolic extract when compared to low polar petroleum ether extract. The minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration were determined against all the test strains. This study provides a basis for the isolation and purification of anti-dermatophytic compounds from the young leaves of S. nigrum.
The present investigation was aimed to study an antiepileptic activity of methanolic extract of Tragia involucrata Linn in mice. In vivo screening models like maximal electroshock-induced convulsion (MES), pentylenetetrazole (PTZ) and picrotoxin (PTX) induced models are used to evaluate the antiepileptic effects of the extracts. The biochemical estimation was done by measuring the lipid peroxidation and reduced glutathione (GSH). In the MES induced convulsion, methanolic extract of Tragia involucrata (METI) at high dose (800 mg/kg body weight), showed high significant inhibition on tonic hind limb extension (THLE, 6.83 ±0.30***) and decrease in duration of stupor period (108.7 ±6.53***). In PTZ and PTX induced model METI (400 mg/kg and 800 mg/kg) showed significant delay on the onset of convulsions, decreased duration of convulsion and reduced mortality significantly. It also showed significant decrease in brain MDA level in lipid peroxidation profile, and increase in the brain glutathione levels in mice against PTZ induced convulsion. The results confirmed that Tragia involucrata Linn possesses dose dependent antiepileptic activity.
Methanolic extract of Artemisia annua L. were evaluated on growth and nutritional efficiency of lesser mulberry pyralid Glyphodes pyloalis (Lepidoptera: Pyralidae) in laboratory conditions (24±1°C, 75±5% RH and 16 L : 8 D). Feeding deterrence index was evaluated by a choice test method on < 24 h fifth instar larvae with 5, 2.5, 1.25 and 0.625 percent of the extract. The result indicated that increasing the concentration resulted in higher deterrence. The extract retarded larval growth significantly (EC50 = 3.63%) and also showed significant effects on the relative growth rate (RGR), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), approximate digestibility (AD) and consumption index (CI). Lipid, protein and carbohydrate in treated insects were significantly reduced compared to the controls. Hence, methanol extract of A. annua could be considered as a strong deterrent which also affects the biochemical metabolism of the target pest. Methanol extract of A. annua has the potential for development as a botanical insecticide.
The composition and effect of heating (60°-110°C) on antioxidative activity in plant oil of rapeseed (RE) and evening primrose (EPE) methanolic extracts were studied. Different phenolics characterized the extracts. The EPE contained about 10-fold higher amount of phenolics (862.2 g/kg extract) than RE; flavanols (683.8 g/kg) and proanthocyanidins (760.1 g/kg) prevailed in the former and phenolic acid derivatives in the latter. The phenolic compounds of both extracts differed with the fenolic acids composition. Sinapic acid prevailed in rapeseed phenolics and protocatechic and gentisic acids in evening primrose phenolics. Both extracts had generally similar antioxidative activity in the temperature ranging from 60°C to 80°C with the oil peroxide values from 3.5 mmol O/kg to 5.0 mmol O/kg compared to the range of 6.0-7.0 mmol O/kg in control oils. The temperatures above 80°C decreased the antioxidative activity of both extracts and even made them to be prooxidative.
Chloroform, butanolic and water fractions from the methanolic extracts of Lamium album L. and L. purpureum L. flowers and different organs of mistletoe (Viscum album L.), namely leaves, stalks and fruits were investigated for the free radical scavenging properties by using colour free radical DPPH • as a stain reagent for dot-blot assay on a T LC plate and two-dimensional thin-layer chromatography (2D-TLC) analysis (2D-TLC-DPPH test) as well as a dye reagent for the spectrophotometric assay. For each plant material, butanolic fractions showed the strongest activity, of which those of the Lamium species were nearly equal to that of the known antioxidant - BHA. According to 2D-TLC chromatography, the phenolic compounds present were responsible for the antiradical activity of the fractions.
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