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Methane in the environment (a review)

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The atmospheric concentration of methane, a potential greenhouse gas, is determined by global balance between sources and sinks. The aim of the paper was to review the recent studies on the natural and anthropogenic sources that are responsible for the increase in the concentration of methane in the atmosphere, focusing on processes of methane formation and oxidation and factors influencing them. Methanotrophic and methanogenic metabolisms are an important part of these researches from the viewpoint of environmental protection. Great variability of soil properties and their interactions affecting the production, consumption and transport of CH4 makes our understanding of these processes still insufficient. Although most research to date has focused on sources of methane emissions, CH4 absorption by oxygenated soil is an important process that significantly reduces emission. The paper describes many soil-related factors affecting methanotrophic activity: particle size distribution, humidity, temperature, pH, oxygen concentration, use of nitrogen fertilisers. Land use has a significant effect on CH4 oxidation in soils, the greatest methnotrophic potential being that of forest soils as compared to meadows, pastures and arable land.
The potential of methanotrophic activity (MTA) has been investigated under labo-ratory conditions in three types of peatland profiles: high (H), transition (T) and low (L) originating from Polesie Lubelskie Region. Selected peat samples differed in respect of pH, TOC, von Post index and moisture. The experiment was conducted at natural moisture (198-719 %w/w) with dif-ferent ranges of both, temperature (5, 10 and 20°C) and CH4 enrichment (1 and 5%v/v). The highest MTA (19.69-155.79 mg CH4kg D.W.-1 d-1) was observed at 20°C. Regardless of temperature, MTA was lower (1.38-51.16 mg CH4 kg D.W.-1 d-1) when peat samples were incubated in atmosphere enriched in 1% than in 5% CH4 v/v (4.75-191.26 mg CH4kg D.W.-1 d-1). Strong influence of tem-perature and sampling sites on MTA was also noted. Total DNA was isolated from the most active (20°C, 5% CH4 v/v) peat samples from each site and the PCR (polimerase chain reaction) amplify-ing of genes pmoA (primers A189f/mb661r) and sequence 16S rRNA (primers Type If /Type Ir and Type IIf/Type IIr) specific for methanotrophic bacteria were carried out. Positive results of PCR with primers of pmoA gene after sequencing confirmed that methanotrophs from L point belong to family Methylococcaceae, while 16S rRNA gene sequences from microorganisms inhabiting H peat demonstrated the highest similarity to genus Methylocystis and Methylosinus.
This paper focuses on the catalytic properties of Ag/SnO₂ for low-temperature methane oxidation. The influences of the metal loading (0.2-20 wt.% Ag), support (Al₂O₃, TiO₂, SiO₂) and methane concentration (0.375-1.5%) in the oxidized mixture were investigated. It was observed that the optimal amount of silver in Ag/SnO₂ was 1 wt.%. Lower or higher amounts cause a loss in catalytic activity. SnO₂ was found to be undoubtedly the best support for the title reaction among all tested oxides. A mechanism of methane oxidation over Ag/SnO₂ was proposed.
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