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This study investigated whether mycorrhizal colonization influences heavy metal uptake by maize. Two experiments were carried out. In the first, 15 commercially available maize varieties cultured on industrial waste substratum and inoculated or not with Glomus intraradices, were treated one week before harvest with EDTA, a chelating agent known to mobilize heavy metals in soil. Estimation of mycorrhizal parameters indicated differences between varieties, but differences between treatments of the same variety generally were not statistically significant. Although EDTA treatment strongly decreased the activity of fungal alkaline phosphatase (indicator of fungal viability), the treatment did not totally eliminate arbuscular mycorrhizal fungi (AMF) from the soil. The appearance of AMF structures within roots was modified in plants cultivated in EDTA-treated soil. Among the heavy metals studied, the highest impact of EDTA treatment on heavy metal uptake in shoots was found in the case of Pb. In most cases, EDTA treatment significantly increased the Pb level in shoots of mycorrhizal plants. Among the samples treated with EDTA, mycorrhizal plants of 6 cultivars showed higher Pb content in shoots than did nonmycorrhizal plants. Significant differences in heavy metal content in plant material were demonstrated between the varieties tested. In the second experiment, one selected cultivar was subjected to high soil Pb concentrations and to EDTA for one week, following cultivation in nonpolluted substratum. In this case, EDTA treatment more strongly influenced Pb uptake by nonmycorrhizal than by mycorrhizal plants. The results indicate the need to carefully screen cultivars as well as microorganism strains to be used in phytoextraction procedures.
We investigated the antioxidant defense mechanism, metal uptake and lipid peroxidation (LPO) levels at different leaf positions in Mentha piperita L. grown in Mn2+-deficient and control conditions. Under manganese deficiency the activity of superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GuaPOX) and the content of ascorbate, chlorophyll, and carotenoid under Mn2+ deficiency were significantly lower than in the control for all leaf positions. SOD activity correlated positively with Mn2+ uptake. Fe2+ uptake was inhibited by Mn2+ deficiency. During early stages of Mn2+ deficiency, M. piperita leaves showed relatively more antioxidant activity and lower LPO. Towards the final stages of the treatment period, comparatively lower SOD, CAT and GuaPOX activity and higher LPO levels accelerated the senescence process.
Accumulation of Cd, Pb, Zn, Cu (HM) by spring barley (Hordeum vulgare L.) from sod podzolic sandy loam and chernozem soils, impacted by heavy metals pollution in the soils, is studied in the article. The aim of study has been to determine spring barley bioaccumulation capacity impacted by the HM pollution with the high level of Cd, Pb, Zn, Cu concentration in soils. The HM concentration diapason caused biomass reduction – the scope of toxic tolerance was obtained. The range of contaminants concentration in soil, which caused the plant biomass reduction, from the beginning to plants death – the scope of toxic tolerance, is the index of a species reaction on selected xenobiotic. It shows: “threshold” concentration of a contaminant that caused a plant biomass inhibition; toxic process development and the correlation between contaminants concentration in soil and/or plant and the plants inhibition; the concentration that caused the plant death. Spring barley accumulation indexes of the studied metals were calculated. Relevant scopes of the plant-uptake index for each metal were calculated. Dynamics of the toxic process development of spring barley as impacted by the pollution in the break-down by studied metals were observed on two different soils. Toxic process dynamic evaluation gives the possibility to simulate concentration of the trace metal in plants, concentration of available forms of these elements in soils, and also contamination level (content of metals) that caused plants height and plant weight reduction by 10%, 50% and 90%.
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