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Recently, calculations of the given parameters of cationic equilibrium in plants, for example Digitalis purpureae, have been very important. For the observed environmental conditions the constants in Czarnowski’s model were as following: flower: 170.64; root: 174.44; leaves: 5.80, and stalk: 266.00. These calculated values are higher in comparison to Scapania undulata, growing in the areas of the Sudety Mountains. The study confirmed that the model may be used to estimate the environmental exposure of the plants.
Background. Fish are often used as indicators of pollution levels in water environments. The omnivorous chub inhabiting rivers of Europe, including their more polluted parts, is a potentially suitable indicator. The information published, however, on the metal accumulation in the tissues of chub living in varyingly polluted waters (both polluted and unpolluted environments) is scarce. The presently reported study was conducted to examine the metal concentrations in selected tissues of chub living in a natural submontane river. Materials and Methods. Sediment samples (0–5 cm layer) and chub, Leuciscus cephalus (L.), were collected from the upper (stations 1–4) and lower (stations 5–8) sections of the Biała Tarnowska River in summer 2003. Fish age was determined from the otoliths. The AAS method was used to determine metal concentrations (Cd, Pb, Cu, and Zn) in sediment and chub tissues (spleen, liver, and muscle). Results. According to the values of the geochemical index (Igeo), the river sediments were not polluted by Pb, Cu, and Zn, while in various degrees polluted by Cd. Despite the low metal concentrations, the mean Cd and Cu concentrations were higher (c. 2× and 4×, respectively) in the upper part of the river compared to the lower section, while concentration of Pb and Zn were similar in the two areas. Chub accumulated the greatest amount of Cu in the liver, while the bulk of Cd, Pb, and Zn was found in the spleen. In the upper part of the river, elevated concentrations of trace elements in selected tissues of chub were found: Cu (spleen, muscle), Cd (spleen), Zn (muscle). The pollution status of the river, on the basis of trace element contents in the sediment, was also determined. Conclusion. The obtained results indicated tissue-specific metal accumulation in chub. Selected chub tissues are sensitive to Cd and Cu contents in the river sediment.
The distribution of heavy metals in sediments of the Nemunas river have been investigated. The most obvious anthropogenic impact has been observed in the middle part of the river. Nevertheless, sediment metal concentrations are within the limits of global, regional and local concentration levels. Particular attention has been paid to the Kaunas Reservoir. Although it is a part of the river, sediment characteristics, including metal distribution, have been found to follow the bottom dynamic zones, as in lakes. Metal concentrations in the Kaunas Reservoir are close to the concentrations in the upstream part of the Nemunas river, without indicating increased contamination with metals due to the lower flow velocity. An unusual Fe/Mn ratio is characteristic to the Reservoir. Fe concentrations confirm the occurrence of an­oxic hypolimnion, whereas Mn concentrations indicate the possibility of Mn transport from the nearshore sediments to the deepest places.
Since the mountains often represent a barrier for the pollutants in many regions, the concentrations of toxic elements in the tissues of alpine animals may remain relatively high and do not decrease in the current times. To estimate heavy metal contamination of alpine ecosystems Snow voles (Chionomys nivalis) are very useful monitors. They are small, easy to catch, have a territory of limited range, fairly short life span and they are closely adjusted to their environment The voles were monthly bait-trapped in the West Tatras, the Western Carpathians, Slovakia. The local population was studied at the Brestova mountain chain (49°13’29.43’’N; 19°40’46.07’’E, 1902 m.a.s.l.). The animals were trapped in September 2009 and from May to November 2010. The global sampling yielded a total of 50 samples (trapped and retrapped individuals). The lead concentrations in the tail vertebrae and the number of micronuclei in peripheral blood were examined. The Pb levels in voles were exceptionally high in overwintering mature animals (16.1 μg g–1dry weight in average) in comparison to young immatures (4.3 μg g–1). Females had significantly higher concentrations of Pb in their bones (13 μg g–1) than males (7.3 μg g–1). Snow vole adults caught in the spring exhibited significantly higher micronuclei frequencies in peripheral blood than immature ones trapped in summer or fall. Given that Pb is bioaccumulated in the diet of voles, this study showed that feeding on winter diet (mosses, lichens) could constitute a major pathway for the entry of Pb into food chain of alpine habitats. The usefulness of Snow voles as biomonitors of environmental contamination in alpine ecosystems was highly recognized.
This paper presents the results of determination of artificial 137cs and natural 40K activity concentrations and some heavy metals in soil samples from the region of one of the main valleys of Tatra National Park (Chochołowska). Our investigation concentrated on 137Cs and heavy metal levels in mountain soil taken from Chochołowska Valley, which revealed great variability in their concentration. The results show con­siderably small amounts of radionuclides 137cs and 40K in the soils. Larger amounts of those elements can be found in the organic surface horizons of the soils. The evaluation of the content of those elements must be based on the bulk density analysis of the soil.
The aim of this study was to detect heavy metal concentrations in the creek which passes through Nigde city garbage dump. In this respect, the soil samples collected in the study area by GPS were analyzed using X-Ray Fluorescence Spectrometer. Traces of heavy metals were observed by using statistical programs on analysis results. Geological observations were taken into consideration. According to the heavy metal concentrations in the Creek, the maximum values were as follows; (mg/ kg) Ti: 4960, V: 520, Cr: 120, Mn: 810, Ni: 460, Cu: 410, Zn: 100 and Pb: 480. The concentration levels of heavy metals in soil caused by garbage dump are listed in the following order; Pb > Cu > V > Ti > Cr > Ni > Zn. High positive correlation were observed between Ti-Mn, V-Ni and Cr-Ni. Medium positive correlations were observed between V-Cr, V-Cu and Ni-Zn. It has been found by chemical analysis data collected from stations established throughout the creek that there is an effect of morphological structure and no geologi­cal anomaly has been formed. According to chemical analysis results, statistical programs give important accuracy. Heavy metals, which give anomaly, differ throughout the creek. The source of these heavy metals was determined as Nigde city garbage dump. For this reason, it was suggested that, in order to refine the study area, a modern, organized storage system must be used instead of wild storage system in storing the urban wastes, and recyclable products must be used in the industry.
The aim of the study was to determine how dairy sewage sludge (DSS) sanitised with coal fly ash affected microbiological properties of soil and level of heavy metals in soil. The experiment was performed under laboratory conditions (pot experiment). The experiment was carried out on grey-brown podzolic soil, formed from heavy loamy sand, of acidic reaction. Pots were filled with 4 kg of soil. The investigations were performed in three replications. Two rates of dairy sewage sludge fertilization: 1 and 2.5% DSS⋅kg-1 of soil were applied. The soil in pots was watered to 60% of the total water capacity and incubated for 4 months. Analyses included assays of the total number of bacteria and fungi, number of cellulolytic bacteria, respiration and dehydrogenase activity and concentration of heavy metals. It was confirmed that dairy sewage sludge had a significant effect on properties of soil. It positively affected microbiological activity of soil. The test doses (1; 2.5%⋅kg-1) of dairy sewage sludge sanitised with lignite ash caused stimulation of the growth of bacteria and fungi in soil. Dairy sewage sludge had an inhibiting effect on the dehydrogenase activity and stimulated the respiratory activity in the soil under study. The incorporation of dairy sewage sludge caused a non-significant increase of heavy metal content in soil, which was much lower than the norms.
The study reports on the amount of lead and cadmium in selected samples of vegetables obtained directly from Warmian-Masurian producers, and commercial samples from a deep-frozen food producer. Concentration of analysed compounds after dry mineralisation of biological material and organic phase extraction (APDC/MBIK) has been determined using the atomic absorption spectrophotometry (AAS) in a UNICAM 939 AA apparatus. There has been significantly high concentration of lead in the samples of cauliflower (0.95 mg/kg) and carrot (0.98 mg/kg). In commercial samples the difference was statistically significant. Whereas significantly higher concentration of cadmium has been detected in spinach both in producer and commercial samples (0.092 and 0.086 mg/kg respectively). Generally, concentration of metals determined was similar in vegetable samples obtained from the suppliers and in commercial ones. The researched vegetables were characterised by lower concentration of particular compounds compared to valid legal regulations.
The aim of our study was to investigate the changes of metal concentrations in stream sediments depending on station and sampling period variation. Sediment samples were collected seasonally from six separate stations selected along Kilicozu Creek (Kirsehir, Turkey), and the Pb, Cr, Cu, Ni, Zn, and Cd concentrations were determined. Stations were selected on the basis of pollution gradient. It was observed that the metal concentration means per annum were Zn> Cr> Ni> Cu> Pb> Cd. Seasonal highest values of heavy metals were observed as follows; Pb (14.4 µg·g⁻¹), Ni (43 µg·g⁻¹), and Cd (6.2 µg·g⁻¹) in autumn, Cr (55.7 µg·g⁻¹) and Zn (71.9 µg·g⁻¹) in summer, and Cu (42.5 µg·g⁻¹) in spring. One-way ANOVA results also showed that there were significant metal concentration changes between stations. Potential metal contamination risk was determined for studied sediment samples. It was observed that Cd contamination exceeded the limit values in this stream sediment. According to the reference values, Zn or Pb contamination in the creek sediment has not reached the effective level. The findings of this study may be useful for further biomonitoring studies.
PAHs (polycyclic aromatic hydrocarbons) are widespread in the natural environment. They are suspected to have mutagenic, carcinogenic and teratogenic effect on our organisms. Production of wholesome and high quality vegetables does not necessarily involve selection of adequate farmlands or substrates used for vegetable growing. Combustion processes are a source of PAHs in the soil and air. Excessive levels of PAHs are accumulated when the balance between their decomposition in soil and supply from other sources is disrupted. The objective of the present research has been to evaluate the effect of anthracene and pyrene on the concentration of selected trace elements in butter lettuce (Lactuca sativa L.), cultivar Vilmorin, grown on substrates with different nutrient abundance. A pot experiment in four replications was carried out twice in the spring of 2007 and 2008. The pots were maintained in a greenhouse at the University of Warmia and Mazury in Olsztyn. Lettuce was grown under the minimum (optimum) and triple (II fertilization level) substrate abundance in nutrients. In the second variant o the experiment, when the nutrient abundance was elevated, nitrogen was split into two doses: 2/3 of the whole rate were added before planting lettuce seedlings, and 1/3 of the rate was introduced 10 days afterwards. In the first variant, when the nutrient abundance was minimal, all of the nitrogen was added in a single dose. Spraying the lettuce plants with anthracene (ANT), pyrene (PYR0 or their mixture started 10 days after planting. Foliar introduction of the tested PAHs continued for 25 days (in the amount of 1.8 cm3 day–1 at a concentration of 100 mg dm–3) until the vegetative season finished. At the same time, an identical total amount of the PAHs was added to soil as aqueous solution, at 7-day intervals, according to the same pattern. Determinations of the concentrations of heavy metals (Cd, Cu, Ni, Cr, Zn, Mn) was performed with the ASA method, having first mineralised (HClO4+HCl+HNO3) the plant material which had been dried at 60oC. The determinations were compared with certified material (CTA-VTL-2). Concentrations of the trace elements in butter lettuce were significantly varied depending on the experimental factors. A more abundant substrate significantly raised the levels of Cd, Zn and Mn, but had no effect on concentrations of Cu, Ni and Cr. The way the PAHs were applied did not have any influence on modifications in the determined levels of the metals. Out of the two analysed PAHs, anthracene, especially when applied to soil, increased the concentrations of Zn nd Cr in lettuce leaves.
Research were carried out in study site located in Stare Bogaczowice near Wałbrzych. The daily drainage runoff measurements and periodical chemical analyses of water samples from selected drainage plots had been made. Concentrations of heavy metals: Cu, Zn, Zn, Pb, Cr i Cd had been measured. Drainage waters do not include signifi cant amounts of heavy metals. Concentration of zinc, which is very common in nature, ranged from 0.018 to 0.675 mg Zn·dm–3, cadmium concentrations was somewhat larger then 0.001 mg Cd · dm–3. In spite of precipitations higher then in the other sites and because of low concentrations, the heavy metal loads had been very small. Regarding lead and nickel load did not exceed 70 g·ha–1·yr–1, and concerning copper, chromium and cadmium it was below 5.0 g·ha–1· ·yr–1. Heavy metals in drainage waters do not make serious threat on surface water body receivers.
We investigated the antioxidant defense mechanism, metal uptake and lipid peroxidation (LPO) levels at different leaf positions in Mentha piperita L. grown in Mn2+-deficient and control conditions. Under manganese deficiency the activity of superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GuaPOX) and the content of ascorbate, chlorophyll, and carotenoid under Mn2+ deficiency were significantly lower than in the control for all leaf positions. SOD activity correlated positively with Mn2+ uptake. Fe2+ uptake was inhibited by Mn2+ deficiency. During early stages of Mn2+ deficiency, M. piperita leaves showed relatively more antioxidant activity and lower LPO. Towards the final stages of the treatment period, comparatively lower SOD, CAT and GuaPOX activity and higher LPO levels accelerated the senescence process.
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