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Effect of meta-topolin in vitro propagation of Pelargonium x hortorum and Pelargonium x hederaefolium cultivars

100%

Wojtania A.

Acta Societatis Botanicorum Poloniae

2010

tom 79

nr 2

101-106

July and September. The most efficient regeneration and axillary multiplication were achieved on the medium supplemented with meta-topolin. The application of BAP caused a lower regeneration potency of explants and resulted in a decrease of shoot quality with every subculture. Four of the six cultivars showed growth inhibition after three months of growth on BAP-medium. The highest multiplication rate (2.7-4.7 depending on genotype) and the high quality of shoots were noted on the medium supplemented with mT (0.5-1.0 mg l-1). It is also very important to note that mT had stimulating effect on organogenesis in P. × hederaefolium and P. × hortorum cultivars over the long term. Moreover, meta-topolin had no after-effect on the growth and inhibition of rooting. Only one cultivar ("Sofie Cascade") rooted better on control medium without auxin. In case of the other cultivars, IBA added in concentrations of 0.01-0.1 mg l-1 had a stimulating effect on root production. The higher level of auxin inhibited root formation, stimulated senescence of shoots and had a negative after-effect on acclimatization in greenhouse conditions.

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1 Acta Societatis Botanicorum Poloniae

1 Wojtania A.

1 2010

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Effect of meta-topolin in vitro propagation of Pelargonium x hortorum and Pelargonium x hederaefolium cultivars