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Cytokine mRNA expression and IFN-gamma production of immunised nematode resistant and susceptible lambs against natural poly-generic challenge

100%

Pernthaner A., Cabaj W., Stankiewicz M., Davies J., Maass D.

Acta Parasitologica

1997

tom 42

nr 3

IL-2, IL-4 and IFN-γ mRNA expression, and production of IFN-γ was examined in mesenteric lymph node cells (MLNC) and CD4⁺ enriched T cell populations of nematode resistant (R) and susceptible (S) line lambs by use of RT-PCR and ELISA. Five R and S line lambs that were immunised by repeated oxfendazole-abbreviated infections and 5 non-immunised R and S line lambs were used. All lambs grazed nematode infected pasture for 107 days. Immunisation enhanced the resistant status in both R and S lambs. MLNC obtained from slaughtered animals were stimulated with Con A or T. colubriformis specific antigen. Non-stimulated MLNC of immunised lambs expressed higher levels of IL-4 mRNA and lower levels of IL-2 mRNA than non-immunised lambs. MLNC of immunised R and S line lambs stimulated with antigen for 24 h expressed detectable amounts of IL-4 mRNA that was not seen in non-immunised controls. CD4⁺ T cell enriched cell populations of immunised R and S lambs and non-immunised R lambs expressed moderate to high levels of IL-4 mRNA. Con A stimulated MLNC of immunised R and S lambs expressed high levels IFN-γ mRNA and produced high amounts of IFN-γ. Lower levels were present in non-immunised controls. The results indicate that R line lambs and immunised S line lambs respond to natural nematode challenge with a predominating IL-4 cytokine response when compared to non-immunised S lambs.

Cytokine mRNA expression and IFN-gamma production in nematode resistant and susceptible line lambs artificially infected with gastro-intestinal nematodes

88%

Pernthaner A., Vlassoff A., Douch P. G. C., Maass D. R.

Acta Parasitologica

1997

tom 42

nr 1

The mRNA expression of the cytokines interleukin (IL)-2, IL-4 and interferon (IFN)-γ and the production of IFN-γ was examined in mesenteric lymph node cells (MLNC) and CD4⁺ enriched cells populations from resistant and susceptible line lambs by use of reverse transcriptase-polymerase chain reactions (RT-PCR) and ELISA. MLNC obtained from lambs that were previously infected with a mixed gastro-intestinal nematode population were stimulated with Con A or Trichostrongylus colubriformis specific antigen. Four weeks after infection MLNC from both line lambs expressed high levels of mRNA coding for IL-2, IL-4 and IFN-γ. MLNC from resistant line lambs when stimulated with antigen for one day had higher mRNA expression of IL-2 and IFN-γ and after three days of culture had higher levels of IL-4 mRNA than MLNC from susceptible line lambs. Antigen stimulated MLNC of resistant line lambs had a higher IFN-γ production than those from susceptible line lambs. These results indicate that in resistant line lambs the secondary in vitro responses of cytokine mRNA and IFN-γ production to nematode specific antigen differs from those of susceptible line lambs.

Ograniczanie wyników

2 Acta Parasitologica

2 Pernthaner A.

1 Cabaj W.

1 Davies J.

1 Douch P. G. C.

1 Maass D.

1 Maass D. R.

1 Stankiewicz M.

1 Vlassoff A.

2 1997

Wyniki wyszukiwania

Cytokine mRNA expression and IFN-gamma production of immunised nematode resistant and susceptible lambs against natural poly-generic challenge

Cytokine mRNA expression and IFN-gamma production in nematode resistant and susceptible line lambs artificially infected with gastro-intestinal nematodes