Wyniki wyszukiwania

1

Brain spectrin binds membrane phospholipids

100%

Diakowski W., Prychidny A., Kwiatkowska J., Kozubek A., Sikorski A. F.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

2

Regulation of the NADPH oxidase activity and anti-microbial function of neutrophils by arachidonic acid

86%

Hii C. S., Ferrante A.

Archivum Immunologiae et Therapiae Experimentalis

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2007

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tom 55

|

nr 2

3

Monitoring of membrane phospholipid scrambling in human erythrocytes and K562 cells with FM1-43 - a comparison with annexin V-FITC

72%

Wrobel A., Bobrowska-Hagerstrand M., Lindqvist C., Hagerstrand H.

Cellular and Molecular Biology Letters

|

2014

|

tom 19

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nr 2

The styryl dye FM1-43 becomes highly fluorescent upon binding to cell membranes. The breakdown of membrane phospholipid asymmetry in ionophore-stimulated T-lymphocytes further increases this fluorescence [Zweifach, 2000]. In this study, the capacity of FM1-43 to monitor membrane phospholipid scrambling was explored using flow cytometry in human erythrocytes and human erythrocyte progenitor K562 cells. The Ca2+-dependent phosphatidylserine-specific probe annexin V-FITC was used for comparison. The presented data show that the loss of phospholipid asymmetry that could be induced in human erythrocytes by elevated intracellular Ca2+ or by structurally different membrane intercalated amphiphilic compounds increases the FM1-43 fluorescence two- to fivefold. The profile of FM1-43 fluorescence for various treatments resembles that of phosphatidylserine exposure reported by annexin V-FITC. FM1-43 detected the onset of scrambling more efficiently than annexin V-FITC. The amphiphile-induced scrambling was shown to be a Ca2+-independent process. Monitoring of scrambling in K562 cells caused by NEM-induced Ca2+-release from intracellular stores and by Ca2+ and ionophore A23187 treatment showed that the increase in FM1-43 fluorescence correlated well with the number of annexin V-FITC-detected phosphatidylserine-positive cells. The results presented here show the usefulness of FM1-43 as a Ca2+-independent marker of dissipation in asymmetric membrane phospholipid distribution induced by various stimuli in both nucleated and non-nucleated cells.

4

Changes in membrane phospholipid biosynthesis induced by chloropromasine and nucleoside analogues: its therapeutic consequences

72%

Staub M., Sumeg R., Sasvari-Szekely M., Simmonds H. A.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

5

The low temperature drying of soybean [ Glycine max ] seeds as a factor reducing injuries caused by exposure to chill during subsequent germination

58%

Dubert F., Filek W.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1995

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tom 43

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nr 1

6

Functions of platelet activating factor [PAF] in mammalian reproductive processes: a review

58%

Kordan W., Strzezek J., Fraser L.

Polish Journal of Veterinary Sciences

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2003

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tom 06

|

nr 1

Platelet activating factor, PAF, (l-0-alkyl-2-acetyl-sn-glycero3-phosphorylcholine) is a naturally occurring compound of membrane phospholipid. The aim of this article was to briefly review current research on the significant role of PAF in mammalian reproductive functions. The involvement of this phospholipid in the female reproductive processes may indicate that it plays an important role in ovarian follicular development, reproductive cycle and pregnancy. A full understanding of PAF functions in sperm motility, capacitation and the acrosome reaction is mandatory to correctly interpret its role in the male reproductive processes. This review also addressed the importance of the mechanism regulating PAF metabolism, PAF-acetylhydrolase (PAF-AH), during the membrane fusion events associated with fertilization.

7

Annexins - calcium- and membrane-binding proteins in the plant kingdom. Potential role in nodulation and mycorrhization in Medicago truncatula

51%

Talukdar T., Gorecka K. M., de Carvalho-Niebel F., Downie J. A., Cullimore J., Pikula S.

Acta Biochimica Polonica

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2009

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tom 56

|

nr 2

199-210

Annexins belong to a family of multi-functional membrane- and Ca2+-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca2+-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant counterparts were discovered only in 1989, in tomato, and have not been thoroughly studied yet. In the present review, we discuss the available information about plant annexins with special emphasis on biochemical and functional properties of some of them. In addition, we propose a link between annexins and symbiosis and Nod factor signal transduction in the legume plant, Medicago truncatula. A specific calcium response, calcium spiking, is an essential component of the Nod factor signal transduction pathway in legume plants. The potential role of annexins in the generation and propagation of this calcium signal is considered in this review. M. truncatula annexin 1 (MtAnn1) is a typical member of the plant annexin family, structurally similar to other members of the family. Expression of the MtAnn1 gene is specifically induced during symbiotic associations with both Sinorhizobium meliloti and the mycorrhizal fungus Glomus intraradices. Furthermore, it has been reported that the MtAnn1 protein is preferentially localized at the nuclear periphery of rhizobial-activated cortical cells, suggesting a possible role of this annexin in the calcium response signal elicited by symbiotic signals from rhizobia and mycorrhizal fungi.

Ograniczanie wyników

Brain spectrin binds membrane phospholipids

Regulation of the NADPH oxidase activity and anti-microbial function of neutrophils by arachidonic acid

Monitoring of membrane phospholipid scrambling in human erythrocytes and K562 cells with FM1-43 - a comparison with annexin V-FITC

Changes in membrane phospholipid biosynthesis induced by chloropromasine and nucleoside analogues: its therapeutic consequences

The low temperature drying of soybean [ Glycine max ] seeds as a factor reducing injuries caused by exposure to chill during subsequent germination

Functions of platelet activating factor [PAF] in mammalian reproductive processes: a review

Annexins - calcium- and membrane-binding proteins in the plant kingdom. Potential role in nodulation and mycorrhization in Medicago truncatula