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Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

100%
Chrobak D., Kizerwetter-Swida M., Rzewuska M., Binek M.
Polish Journal of Veterinary Sciences
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2011
|
tom 14
|
nr 2
A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius- specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.
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Antibiotic resistance patterns and occurrence of mecA gene in Staphylococcus intermedius strains of canine origin

84%
Kizerwetter-Swida M., Chrobak D., Rzewuska M., Binek M.
Polish Journal of Veterinary Sciences
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2009
|
tom 12
|
nr 1
We have evaluated 102 Staphylococcus intermedius isolates of canine origin for susceptibility to antimicrobial primary agents, i.e. penicillin, amoxicillin, amoxicillin with clavulanic acid, cefuroxime, trimethoprim/sulfonamides, neomycin, streptomycin, gentamicin, norfloxacin, tetracycline, vancomycin, erythromycin and secondary agents, i.e. chloramphenicol, ciprofloxacin, lincomycin, teicoplanin, rifampicin, imipenem, mupirocin. Antimicrobial sensitivity was examined using the disk diffusion method and performed according to NCCLS quidelines. Methicillin resistance was detected using the disk diffusion method with oxacillin, and the occurrence of mecA gene was detected by PCR. Resistance to streptomycin, penicillin, amoxicillin, neomycin, followed by tetracycline was predominant. From 14 mecA-positive strains, 12 were multidrug-resitant, and the remaining two showed atypical susceptibility. One strain resistant to oxacillin in the disc diffusion method was mecA-negative, suggesting a different mechanism of resistance. Our results indicate that the emergence of S. intermedius resistance to methicillin may be underestimated. In case of clinical multidrug-resitant S. intermedius isolates, resistance to methicillin should be considered.
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Characterisation of Staphylococcus aureus strains isolated from cows' milk

84%
Bystron J., Bania J., Lis E., Molenda J., Bednarski M.
Bulletin of the Veterinary Institute in Puławy
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2009
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tom 53
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nr 1
59-63
The aim of the study was to characterise the Staphylococcus aureus strains isolated from unprocessed cow's milk for staphylococcal superantigenic toxin genes (sea-selu and tst), to examine the presence of mecA gene and to determine the type of staphylococcal protein A (spa) gene. The type of the gene was determined for all 68 Staphylococcus aureus strains, based on the polymorphism of a number of repeated motifs found in the region X of spa. The genes forming the egc 1 cluster, i.e. seg, sei, selm, seln, and selo were found to be the most prevalent (29.4%). Three isolates (4.41%) carried seh and one (1.47%) the seb/selk genes. No tst and mecA genes were found in the examined S. aureus. Spa types containing three and six to 14 repeats were found in the studied S. aureus population. Strains harbouring eight, ten, and twelve repeats were most frequent (33.82%, 29.41%, and 13.23%, respectively).
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