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1

Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

100%
Jastrzebski Z., Zychowska M.
Baltic Journal of Health and Physical Activity. The Journal of Gdansk University of Physical Education and Sport
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2014
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tom 06
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nr 4
Background: The aim of the study was to designate changes in the expression of HSPA1A, HSPB1 and LDHb in elite rowers after completing a test “till exhaustion” on a rowing ergometer. Finally, we searched for the answer whether there are significant correlations between the expression of the genes and anaerobic threshold (AnT) or the maximal oxygen uptake (VO2max). Material/Methods: The research was conducted on the sample of 9 Polish lightweight male rowers (23.7 ±3.77 yrs, 72.7 ±1.76 kg, 183.6 ±4.58 cm). To determine AnT and VO2max, the subjects performed the test “till exhaustion” with an increasing load on a rowing ergometer. Directly before and after the test, blood samples were collected from the ulnar vein in order to isolate genetic material. RNA was extracted from white cells of venous blood by the chemical method. 2 µg RNA for the reverse transcription was used and the expression of HSPA1A, HSPB1 and LDHb was determined by Real time PCR reaction. To assess the intensity of expression, the ∆∆Ct method was used. Results: The study showed an increased expression of HSPA1A and HSPB1 and a decreased one of LDHb. Moreover, post-training changes of the genes activity in white blood cells occurred immediately and could be determined directly after the termination of exertion. Conclusions: No significant correlations between the expression of the genes and anaerobic threshold (AnT), maximal oxygen uptake (VO2max) were stated.
2

Spirometric and ergospirometric evaluation of wheelchair rugby players

84%
Domaszewska K., Szymczak L., Krysciak J., Pospieszna B., Laurentowska M.
Trends in Sport Sciences
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2013
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tom 20
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nr 2
Introduction. Cervical spinal cord injury is one of most common human body deficiencies. Quadriplegia affects not only the mass of paralyzed muscles, but also disrupts physiological exercise adaptation mechanisms. One of the few sports suitable for individuals with cervical spine impairment is wheelchair rugby. Professional athletes display a higher exercise capacity than untrained people with a similar degree of spinal damage. The reduction of aerobic capacity in individuals with cervical spinal cord injury is multifactorial. In addition to cardio-pulmonary mechanisms limiting the exercise capacity, the decrease in active muscle mass leads to the rapid development of tissue hypoxia. Material and Methods. 14 members of the Polish National Wheelchair Rugby Team were recruited for the study. The male players aged 20-40 years with cervical spinal cord injuries underwent spirometric and ergospirometric tests. Results. The mean values of spirometric parameters were: VC 3.9 ± 0.71 l (71.3% predicted), ERV 0.9 ± 0.33 l (60.7% predicted), VE 12.6 ± 6.34 4 l/min, Bf 18.3 ± 4.72 l/min, VT 0.7 ± 0.20 l, FVC 4.05 ± 0.69 l/min (76.3% predicted), FEV₁,₀/FVC 92.2 ± 7.10% (113.45% predicted), MVV 141.7 ± 24.59 l/min (97.15% predicted). The mean value of peak oxygen consumption during exercise was 1.31 ± 0.30 l/min (17.8 ± 4.99 ml/kg/min) achieved within 11.8 ± 3.51 min. The mean maximal workload was 42.5 ± 13.99 W. During the test only 10 players reached the anaerobic threshold (AT) intensity. The mean workload at AT was 36 ± 10.62 W, and VO2 max at AT was 0.9 ± 0.26 l/min (15.5 ± 4.17 ml/kg/min). Conclusions. The results of spirometric tests indicate the presence of mild and medium restrictive pulmonary changes in 8 out of the studied players of the Polish National Wheelchair Rugby Team. The physiological parameters obtained during the exercise test indicate a higher aerobic capacity of surveyed athletes in comparison with untrained quadriplegics.
3

Difference in maximal oxygen uptake (VO2max) determined by incremental and ramp tests

84%
Czuba M., Zajac A., Cholewa J., Poprzecki S., Roczniok R.
Studies in Physical Culture and Tourism
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2010
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tom 17
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nr 2
4
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Spirometric values and aerobic efficiency of children and adolescents with hearing loss

84%
Zebrowska A., Zwierzchowska A.
Journal of Physiology and Pharmacology. Supplement
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2006
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tom 57
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nr 4
443-447
Vital capacity (VC), forced expiratory volume in 1 s (FEV1), peak expiratory flow (PEF), mean forced expiratory flow (FEV25-75), and maximum voluntary volume (MVV) were measured in 36 girls and 36 boys with hearing loss and compared with the same number of normal healthy children, all subjects were aged 10-16 years. They participated in an exercise test to calculate VO2 max in order to determine their physical efficiency. We found that all spirometric indices tended to be lower in deaf children, in all age-groups studied and irrespective of gender, compared with their hearing counterparts; the differences assumed significance with respect to PEF and MVV (P<0.05). Moreover, some deaf children had an appreciably lower level of VO2max compared with hearing children. Our results demonstrate that sensory deprivation of deaf children affects functional capabilities of the respiratory system.
5

Maximal oxygen uptake is associated with the snp 13470 G>C polymorphism of the mitochondrial NADH dehydrogenase subunit 5 gene (mtND5) in Caucasians from Poland

67%
Gronek P., Holdys J., Krysciak J., Stanislawski D.
Trends in Sport Sciences
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2013
|
tom 20
|
nr 4
Introduction. Physical performance displays a great interindividual variance in both general population and among well-rained athletes. Genetic factor has an important contribution in this variance. The aim of this study was to examine the association between maximal oxygen uptake and genetic variants of mitochondrial NADH dehydrogenase subunit 5 gene (mtND5) in Caucasians from Poland. Material and Methods. The studies were carried out in a group of 154 men and 85 women, professional athletes representing various sports and fi tness levels and students of the University of Physical Education in Poznań. Physiological and molecular procedures were used, i.e. direct measurement of maximal oxygen uptake (VO₂max) and SNP 13470 G>C polymorphism of the mitochondrial NADH dehydrogenase subunit 5 gene (mtND5) was determined by restriction fragments length polymorphism (PCR-RFLP). Results. We have found that maximal oxygen uptake is associated with BamHI+/+ homoplasmic variant of the mtND5 gene in Caucasians from Poland. We have also observed positive infl uence of BamHI+ allele on level of maximal oxygen uptake (VO₂max).
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