Wyniki wyszukiwania

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In-silico prediction and observations of nuclear matrix attachment

100%

Platts A. E., Quayle A. K., Krawetz S. A.

Cellular and Molecular Biology Letters

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2006

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tom 11

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nr 2

The nuclear matrix is a functionally adaptive structural framework interior to the nuclear envelope. The nature and function of this nuclear organizer remains the subject of widespread discussion in the epigenetic literature. To draw this discussion together with a view to suggest a way forward we summarize the biochemical evidence for the modalities of DNA-matrix binding alongside the in-silico predictions. Concordance is exhibited at various, but not all levels. On the one hand, both the reiteration and sequence similarity of some elements of Matrix Attachment Regions suggest conservation. On the other hand, in-silico predictions suggest additional unique components. In bringing together biological and sequence evidence we conclude that binding may be hierarchical in nature, reflective of a biological role in replicating, transcribing and potentiating chromatin. Nuclear matrix binding may well be more complex than the widely accepted simple loop model.

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Sequences flanking rat hsp70.1 gene contain motifs specific for matrix attached regions and form complexes with nuclear matrix proteins in vitro

75%

Widlak P., Rogolinski J., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

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Functions of the chicken lysosyme 5' MAR and MAR binding proteins

75%

Stratling W. H., Weitzel J. M., Buhrmester H., Phi-Van L.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

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Interactions of matrix attachment region of DNA with the matrix proteins from the copper preincubated liver nuclei

63%

Widlak P., Rogolinski J., Rzeszowska-Wolny J.

Acta Biochimica Polonica

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1995

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tom 42

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nr 2

Preincubation of rat liver nuclei with copper ions influenced the stability and protein composition of the nuclear matrices isolated by a "high salt" method. Also the specific interaction between matrix proteins and the kappa Ig matrix attachment region of DNA was affected.

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Effect of nuclear matrix attachment regions on transgene expression in tobacco plants

63%

Nowak W., Gawlowska M., Jarmolowski A., Augustyniak J.

Acta Biochimica Polonica

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2001

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tom 48

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nr 3

Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on reporter gene expression in tobacco plants. The synthetic MAR was a multimer of a short sequence from the MAR 3 end of the immunoglobulin heavy chain (IgH) enhancer. This sMAR sequence was used to flank the β-glucuronidase (GUS) reporter gene within the T-DNA of the binary vector pBI121. Vectors with or without the sMARs were then used to transform tobacco plants by Agrobacterium tumefaciens. Transgenic plants containing the sMAR sequences flanking the GUS gene exhibited higher levels of transgene expression compared with transgenic plants which lacked the sMARs. This effect was observed independently of the position of the sMAR at the 5 side of the reporter gene. However, variation of the detected transgene expression was significant in all transformed plant populations, irrespective of the construct used.

Ograniczanie wyników

3 Cellular and Molecular Biology Letters

2 Acta Biochimica Polonica

2 Rogolinski J.

2 Rzeszowska-Wolny J.

2 Widlak P.

1 Augustyniak J.

1 Buhrmester H.

1 Gawlowska M.

1 Jarmolowski A.

1 Krawetz S. A.

1 Nowak W.

1 Phi-Van L.

1 Platts A. E.

1 Quayle A. K.

1 Stratling W. H.

1 Weitzel J. M.

1 2006

1 2001

2 1997

1 1995

In-silico prediction and observations of nuclear matrix attachment

Sequences flanking rat hsp70.1 gene contain motifs specific for matrix attached regions and form complexes with nuclear matrix proteins in vitro

Functions of the chicken lysosyme 5' MAR and MAR binding proteins

Interactions of matrix attachment region of DNA with the matrix proteins from the copper preincubated liver nuclei

Effect of nuclear matrix attachment regions on transgene expression in tobacco plants