Wyniki wyszukiwania - AGRO

1

Sources of the autonomic fibers to the mammary gland in pig

100%

Boratynski Z., Flieger S., Welento J.

Folia Morphologica

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1996

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tom 55

|

nr 4

2

Involvement of microtubules in the transport of the secretion in secretory cells of the mammary gland

88%

Sporniak M., Perissel B.

Folia Histochemica et Cytobiologica

|

1986

|

tom 24

|

nr 4

3

Development of the mammary glands in female mink from weaning through first lactation

88%

Moller S. H.

Zeszyty Naukowe. Przegląd Hodowlany

|

1996

|

tom 27

4

Development of the mammary glands during gestation and lactation in mink in relation to feeding intensity

88%

Brzozowski M., Moller S. H.

Zeszyty Naukowe. Przegląd Hodowlany

|

1996

|

tom 27

5

Evaluation of mammary gland development in primiparous sows based on morphometric measurements

75%

Rzasa A., Poznanski W., Procak A., Akincza J.

Annals of Animal Science. Supplement

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2005

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nr 1

6

Relationship of bacteriological status of the mammary gland to lactose level in milk yield

75%

Sender G., Bassalik-Chabielska L.

Acta Microbiologica Polonica

|

1990

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tom 39

|

nr 1-2

7

Quantification of bovine mammary stem/progenitor cells by laser scanning and flow cytometry

75%

Osinska E., Gajewska M., Majewska A., Motyl T.

Animal Science Papers and Reports

|

2015

|

tom 33

|

nr 1

Due to the presence and activity of mammary stem cells (MaSC), growth and remodelling of mammary gland during puberty and lactation cycles is possible. In this study the number of putative mammary stem/progenitor cells was examined in 20 months old non-pregnant Holstein-Friesian heifers. Cells were double-stained with fluorescent dye-conjugated antibodies against stem cell antigen-1 (Sca-1) and fibronectin type III domain containing 3B (FNDC3B), and were analysed Rusing scanning cytometry and flow cytometry. Nuclei were counterstained with Hoechst 33342. Scanning and flow cytometry revealed 2.43±0.32% and 1±0.37% of MaSC in total cell number, respectively.Sca-1posFNDC3Bpos cells did not express estrogen receptor (ERα), confirming their undifferentiated phenotype. In conclusion, scanning cytometry is a preferable method for evaluation of the number and localization of MaSC in situ, whereas, flow cytometry with cell sorting enables further genomic and biochemical analyses of isolated cells.

8

Apoptosis and autophagy induced by TGF-beta1 in bovine mammary epithelial BME-UV1 cells

75%

Gajewska M., Gajkowska B., Motyl T.

Journal of Physiology and Pharmacology. Supplement

|

2005

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tom 56

|

nr 3

143-157

Mammary gland growth and involution is based on a dynamic equilibrium between proliferation and apoptosis of mammary gland epithelial cells (MEC). TGF-ß1 is an important antiproliferative and apoptogenic factor for mammary gland epithelial cells, acting in auto/paracrine matter and thus considered an important local regulator of mammary tissue involution. So far the studies on mammary gland involution concerned only apoptosis as a type I of MEC programmed cell death (PCD). Autophagy is known to be type II of PCD and this paper is the first, supporting evidence for the TGF-ß1-induced autophagy in bovine mammary epithelial cell line BME-UV1, as a distinct to apoptosis type of PCD. Laser scanning cytometry and confocal microscopy were used for analysis of MAP1 LC3 and Beclin1 expression - two proteins considered being the most reliable biochemical markers of autophagy. The significant increase of MAP1 LC3 and Beclin1 expression in cells treated with TGF-ß1 (2 ng/ml) was observed. Ultrastructural observation in electron microscopy revealed that autophagy is not only alternative, but also complementary to apoptosis type of cell death in TGF-ß1-treated bovine MEC. It was manifested by typical morphological features of apoptosis (cell shrinkage, margination and condensation of chromatin) and autophagy (autophagosomes, autophagic vacuoles) in the same cell.

9

Microscopic-electron examinations of the influence of the selected antibiotics on the secretory cells of the mammary gland

75%

Cegielski M.

Folia Histochemica et Cytobiologica

|

1986

|

tom 24

|

nr 4

10

Vasoactive intestinal polypeptide [VIP] - immunoreactive nerve fibres in the mammary gland of the pig

75%

Franke-Radowiecka A.

Folia Morphologica

|

2003

|

tom 62

|

nr 3

267-270

Immunohistochemical studies have been performed to investigate the coexistence of VIP with dopamine-β-hydroxylase (DβH), vesicular acetylcholine transporter (VAChT), somatostatin (SOM) or neuropeptyd Y (NPY) within nerve fibres supplying the immature mammary gland in the pig. Generally, a moderate number of the VIP-immunoreactive (VIP-IR) nerve fibres were located in the nipple and parenchyma of the gland. VIP-IR fibres surrounded smooth muscle cells (SMC), blood vessels (BV) and lactiferous ducts (LD). Double-labelling immunohistochemistry revealed that some of VIP-IR nerve fibres also contained immunoreactivity to DβH. VIP/DβH-IR nerves were associated with BV and SMC and single fibres were observed around the LD in both nipple and parenchyma of the gland. VIP/VAChT-IR nerve fibres were not observed. The majority of VIP-IR fibres associated with SMC were also SOM-IR. Less numerous VIP/SOM-IR fibres supplied the BV and were located around the LD of the gland. A small number of VIP-IR nerves also displayed immunoreactivity to NPY. VIP/NPY-IR nerve fibres supplied the BV of the gland.

11

Detection of gonadotropin-releasing hormone and somatostatin-14 containing cells in the mammary gland of guinea pigs

75%

Gencer T. B., Yaman M., Bayrakdar A.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 10

1193-1195

The existence of numerous neuropeptides in milk is well established. It is still unclear whether these neuropeptides are produced by the mammary gland or that the gland concentrates them from the general circulation. The aim of the study was to examine the possible localization of these neuropeptides in the mammary gland of guinea pigs at different physiological states by immunohistochemistry. Specific primers have been used for the somatostatin, vasoactive intestinal polypeptide, neurotensin, cholecystocinin, oxytocin, gonadotropin-releasing hormone and growth hormone. Among all the neuropeptides that have been examined gonadotropin-releasing hormone and somatostatin immunoreactivity were found in the mammary gland of lactating guinea pigs, but not in virgin and pregnant guinea pigs. Immunoreactivity was observed in the epithelial cells that compose the secretory alveoli and in the secretory material

12

Microscopic-electron experimental examination of mastocytes in the mammary gland under the ifluence of the chosen factors

75%

Zarzycki J., Jagoda E., Krajewska B.

Folia Histochemica et Cytobiologica

|

1986

|

tom 24

|

nr 4

13

Myoepithelial cell and its function

75%

Sopel M., Lis A.

Folia Histochemica et Cytobiologica. Supplement

|

1996

|

tom 34

|

nr 1

14

Leptin does not seem to influence glucose uptake by bovine mammary explants

75%

Accorsi P. A., Gamberoni M., Isani G., Govoni N., Viggiani R., Monari M., De Ambrogi M., Munno A., Tamanini C., Seren E.

Journal of Physiology and Pharmacology

|

2005

|

tom 56

|

nr 4

Leptin, a protein produced and secreted by adipocytes, is know to regulate food intake and whole-body energy metabolism, but knowledge about its possible effect in bovine mammary gland is scarce. Leptin may be involved in the regulation of glucose transport even though this effect at the tissue level remains controversial. Once uptaken by the mammary gland, glucose is utilised in several ways but the majority, about 60-70%, is drained for lactose synthesis. This study was aimed at investigating the effect of leptin on glucose regulation in bovine mammary gland. We have examined the effects of leptin on the expression of GLUT1 mRNA, pyruvate kinase (PK) as well as glucose-6-phosphate dehydrogenase (G6PDH) activity. Treatment of mammary gland explants with recombinant leptin did not influence glucose assimilation, pathway transport (GLUT1 mRNA) and glucose metabolism (PK and G6PDH) in this tissue. The results from this study seem to exclude an involvement of leptin in glucose uptake and metabolism in bovine mammary gland.

15

Number of calf head-strikes against the udder in the natural sucking process

75%

Juszka H.

Annual Review of Agricultural Engineering

|

1996

|

tom 01

16

Mast cells in the mouse mammary gland - correlation with the development of lactiferous structures

75%

Szewczyk G., Szukiewicz D., Zaczek R., Maslinska D.

Folia Biologica

|

2000

|

tom 48

|

nr 1-2

17

Sympathetic nerve centres for the mammary gland in pig

75%

Boratynski Z., Flieger S.

Acta Scientiarum Polonorum. Medicina Veterinaria

|

2002

|

tom 01

|

nr 2

18

Apoptosis and autophagy in involuting bovine mammary gland is accompanied by up-regulation of TGF-beta1 and suppression of somatotropic pathway

75%

Zarzynska J., Gajkowska B., Wojewodzka U., Dymnicki E., Motyl T.

Polish Journal of Veterinary Sciences

|

2007

|

tom 10

|

nr 1

Regulation of mammary gland remodeling during the lactation cycle in cattle still remains unclear. The present study focused on the role of TGF-ß1 and somatotropic pathways proteins in control of the switch between survival and death of bovine mammary epithelial cells (MEC). Expressions of TGF-ß1, TGF-ßRII, IGF-IRα, IGF-IRß, GH-R, IGFBP-3, -4, and -5 in mammary tissue explants in Holstein-Fresian heifers (n=7) and cows (n=23) in early lactation (1-100 day), late lactation (200-260 day) and drying off (280-340 day) were compared with biochemical indices of apoptosis (caspase 3, 89 kDa fragment of PARP) and autophagy (Beclinl). The results revealed that an increase in apoptosis during the dry period was accompanied by highly significant increases in TGF-ß1 and TGF-ßRII expression. Beside biochemical markers, typical morphological features of apoptosis, such as cell shrinkage, separation from the neighboring cells and condensation of chromatin were observed. TGF-ß1 expression and induction of apoptosis was facilitated by the suppression of somatotropic pathway during drying off, manifested with down-regulation of GH-R and IGF-IRα, and up-regula- tion of IGFBP-4 and -5. This is the first report describing autophagy in the bovine mammary gland. Similarly to apoptosis, the intensity of autophagy was the highest in the dry period, as shown by increased expression of Beclinl and morphological features, e.g. autophagosomes, autophagic vacuoles. Autophagy observed in the involuting mammary tissue could be the natural cell defense against transient undernourishment and action of apoptogenic peptides (e.g. TGF-ß1, IGFBPs), thus maintaining cellular homeostasis in the dry period.

19

Involvement of CD8plus cells in protective mechanisms in canice mammary adenocarcinomas [short communication]

75%

Nowak M., Madej J. P., Rossowska J., Madej J. A.

Bulletin of the Veterinary Institute in Puławy

|

2007

|

tom 51

|

nr 3

The study aimed at demonstration of involvement of CD8+ cells in protective mechanisms in canine mammary gland carcinomas. Material for the studies involved metastases to lungs sampled at autopsy from crossbreed bitches aged 11 to 13 years. The tumours were verified histopathologically as adenocarcinomas. The presence of CD8+ cells infiltrating the examined tumour tissues was estimated in cryo-sections by immunofluorescence methods. Parental tumours free of metastases were accompanied by slight infiltration of CD8+ cells. In the case of metastasing tumours, and especially in their pulmonary metastases, the increased number of CD8+ cells was observed. Taken together, metastasing potential of canine mammary adenocarcinomas was positively correlated with the number of CD8+ cells in the tumour stroma.

20

The role of GABA-ergic system in human mammary gland pathology

75%

Mazurkiewicz M., Kleinrok Z., Grzybowska-Szatkowska L.

Folia Histochemica et Cytobiologica. Supplement

|

2001

|

tom 39

|

nr 1

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