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Treatments of broad bean and maize seedlings with fluometuron, atrazine or rimsulfuron affected some parameters of oxidative stress. Fluometuron significantly reduced activity of Hill reaction (PSII), chlorophyll a+b contents and activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) in leaves of both species and significantly increased contents of H2O2, lipid peroxides and carbonyl groups during the whole experiment. There were, moreover, significant inhibitions in activities of superoxide dismutase (SOD; E.C. 1.15.1.1), catalase (CAT; E.C. 1.11.1.6), ascorbate peroxidase (APX; E.C. 1.11.1.11) and guaiacol peroxidase (GPX; E.C. 1.11.1.7). Response to atrazine was, to some extent, similar to fluometuron throughout the entire experiment in broad bean and up mostly to the 12th day of the experiment in maize. The herbicide effect was more pronounced in broad bean than maize. These results point to indicate an occurrence of oxidative stress in both species by fluometuron and only in broad bean by atrazine. The increase in H2O2 content accompanied with drop in activities of SOD, CAT and peroxidases indicates a decline in its detoxification rather than increase in its synthesis. On the contrary, rimsulfuron seemed to have no effect on most of the tested parameters although there were transient significant increases in H2O2, lipid peroxides and carbonyl groups as well as activities of SOD, CAT, APX and GPX. These findings, based on the recovery in oxidative stress, indicate that fluometuron is involved in oxidative stress generation in both species but atrazine only in broad bean while rimsulfuron is not in both species.
A study on reduced tillage effect on the micronutrients (B, Cu, Fe, Mn, Mo, Zn) content in two horizons (0-10 cm and 10-20 cm) of the arable layer of sandy soil in the maize monoculture was carried out. Micronutrient supply of maize plants and yielding was also tested. An increase of soil acidity in the 0-10 cm horizon of soil under the reduced tillage was stated. It was accompanied by the increased micronutrient content in comparison with both the deeper, 10-20 cm, horizon under the same two systems and the respective contents in the topmost horizon (0-10 cm deep) under the conventional tillage. This exerted an influence on micronutrient content in plants. A significant increase in the content of Cu and Zn alongside a decrease in the amounts of B, Mn, Fe and Mo in vegetative organs and grain of maize were observed under no-plough tillage. Significantly depressed maize grain yields occurred in the objects under no-plough tillage suggests that the changes of micronutrients contents in soil and maize plants could be one of the reasons. The results obtained indicate the necessity of microelement fertilization of maize monoculture especially when the reduced tillage is applied.
The combination effects of water stress and gibberellic acid (GA₃) on physiological attributes and nutritional status of maize (Zea mays L. cv., DK 647 FI ) were studied in a pot experiment. Maize plants were grown in the control (well watered WW) and water stress subj ected to treated both water stress and two concentrations of gibberellic acid (GA₃ 25 mg·L⁻¹,50 mg·L⁻¹). WS was imposed by maintaining the moisture level equivalent to 50 % pot capacity whereas the WW pots were maintained at full pot capacity. Water stress reduced the total dry weight, chlorophyll concentration, and leaf relative water content (RWC), but it increased proline accumulation and electrolyte leakage in maize plants and appears to affect shoots more than roots. Both concentrations of GA₃ (25 and 50 mg·L⁻¹) largely enhanced the above physiological parameters to levels similar to control. WS reduced leaf Ca2+ and K+ concentrations, but exogenous application of GA₃ int creased those nutrient levels similar or close to control. Exogenous application of GA₃ improved the water stress tolerance in maize plants by maintaining membrane permeability, enhancing chlorophyll concentration, leaf relative water content (LRWC) and some macro-nutrient concentrations in leaves.
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Selenium is an essential element for humans, animals and some species of microorganisms. In higher plants, however, the role of selenium is still unclear. Because selenium enrichment may influence the nutrient balance of plants, a study was done to test the effects of selenite-Se (Na2SeO3⋅5H2O) on selected macronutrients content in maize (Zea mays L. var. saccharata Kcke. cv. Złota Karłowa) seedlings. Plants were grown in Hoagland I nutrient solution (pH 6,2) amended with selenite at 0 (control), 5, 25, 50 and 100 μmol⋅dm-3 for 14 days. The dry weight of the shoots was then analyzed for phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) content. Phosphorus and calcium content increased, while potassium content decreased with increasing selenium treatments. No significant differences were found for magnesium level. Plant growth was affected by excessive selenium concentration. At low concentration (5 μmol⋅dm-3), selenium tended to stimulate the plant growth and the root elongation but at higher concentrations (50 and 100 μmol⋅dm-3) the dry mass accumulation and root tolerance index severely decreased. The study revealed that disturbances of growth and reduction of plant’s biomass at the presence of high selenium concentrations in the nutrient solution may have resulted from the disturbance of mineral balance of plants, namely accumulation of large amounts of calcium and phosphorus in shoot tissues.
Ability of five strains of Trichoderma pseudokoningii (antagonists) to suppress radial growth of Fusarium verticillioides (Sacc.) Nirenberg (=Fusarium moniliforme Sheldon) was examined i n vi tro. These were T. pseudokoningii strain1 (IMI 380933), strain2 (IMI 380937), strain3 (IMI 3809 39), strain4 (IMI 380940) and strain5 (IMI 380941). Each strain was paired with pathogen by inoculating at opposite ends of 9 cm petri plates using three pairing methods. Gradings were assigned to varied growth inhibition of pathogen by antagonists and analysed using GLM procedure (SAS). Growth suppressionof F. verticillioides by all strains of T. pseudokoningii was significantly different (R2 =0.98, p=0.05) from control in all pairing methods. It differed significantly (p>0.0003) among the strains in all pairing methods. Growth suppression also differed significantly among (p>0.0001) and within (p>0.018) pairing methods. Growth suppression was best when antagonists were inoculated before pathogen. Suppression mechanisms include mycoparasitism and competition for space and nutrients. T. pseudokoningii strains 3 and 4 had the best (p=0.05) growth suppressionof F. verticillioides and could be used as biocontrol agents for endophytic F. verticillioides in maize plant. This experiment was conducted in the search for resident microorganisms that might be capable of checking F. verticillioides withinmaize plant by competitive exclusion in subsequent experiments.
The objective of the performed investigations was to isolate pathogenic fungi from contaminated maize cobs, to assess the appearance of maize cob fusariosis and to determine grain contamination with deoxynivalenol in the cultivation of genetically modified maize containing a gene resistance against European corn borer (Ostrinia nubilalis Hbn) as well as selected non-modified cultivars. The plant material comprised the following genetically modified maize cultivar: DKC 3421 YG (MON 810) and non-modified cultivars obtained from Smolice Plant Breeding Ltd., IHAR Group: Junak (FAO 210–220), Prosna (FAO 220), SMH (FAO 230), Baca (FAO 220). Prior to harvesting, the occurrence of maize cob fusariosis was determined in the 89 (BBCH) developmental ripening stage. Microbiological assessment was carried out on grains selected from cobs characterized by various pathological symptoms. In 2008, a total of 133 isolates was obtained from the examined samples of infected maize plants, of which 51 isolates were species-identified, while in 2009, the total of 123 isolates were determined, of which 63 were species-identified. In both experimental years, the majority of isolates contained fungi from the Fusarium genus. The performed analysis of mean levels of cob contamination by fusarioses revealed that DKC 3421 YG (MON 810) and SMH (FAO 230) cultivars showed the smallest levels of contamination as well as the lowest percent of cob contamination per plant, while Junak (FAO 210–220) and Baca (FAO 220) cultivars were characterized by the highest degree of contamination. The lowest deoxynivalenol concentrations were determined in years 2008 and 2009 in the case of the DKC 3421 YG (MON 810) cultivar, whereas Prosna (FAO 220) cultivar was characterized by the highest deoxynivalenol concentration.
Pot experiments were conducted to evaluate the possible roles of nitrogen fixation and/or enhanced mineral uptake by Azospirillum lipoferum and Bacillus polymexa inoculation in improving salt tolerance of maize plants. Plants were inoculated and grown under salt stress (osmotic potential: -0.3, -0.6, -0.9 and -1.2 Mpa). Both microorganisms were able to fix nitrogen up to -0.9 Mpa salinity level accompanied with increased total N-yield compared with the control plants. In order to investigate the role of bacterial inoculation on enhanced mineral uptake, the growth and some physiological parameters of inoculated plants were compared with plants fertilized by K and P foliar application. Plant inoculation with the N₂-fixers or plant spraying with KH₂PO₄ resulted in an increase in fresh and dry matter as well as water content of plants. Treated plants exhibited changed plant mineral content which was associated with increased Mg/K and decreased P/K, Ca/K and Na/K ratios. This was accompanied by accumulation of soluble sugars, amino acids in shoots and roots of plants resulting in a concomitant increase in the osmotic potential of the cell sap as a possible mechanism of adaptation to salinity.
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