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Systemic treatment by inhalation of macromolecules - principles, problems, and examples

100%
Siekmeier R., Scheuch G.
Journal of Physiology and Pharmacology. Supplement
|
2008
|
tom 59
|
nr 6
53-79
2

An approach based on diffusion to study ligand-macromolecule interaction

100%
Housaindokht M. R., Bahrololoom M., Tarighatpoor S., Mossavi-Movahedi A. A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 3
A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30°C. The binding iso­therm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.
3

Viewing nuclear pore contraction with the atomic force microscope

100%
Rakowska A., Schneider S. W., Danker T., Schuricht B., Oberleithner H.
Cellular and Molecular Biology Letters
|
1997
|
tom 02
|
nr 2
4

Towards proto-cells: Primitive lipid vesicles encapsulating giant DNA and its histone complex

100%
Nomura S. I. M., Tsumoto K., Yoshikawa K., Ourisson G., Nakatani Y.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr 2
5

Factors improving the accuracy of determination of 15N relaxation parameters in proteins

100%
Zhukov I., Ejchart A.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 3
A number of factors at all stages of data processing which affect the accuracy of determination of 15N relaxation parameters in 15N-labeled proteins is discussed. Methods which allow to improve accuracy of the determined parameters are presented using data obtained for Cucurbita maxima trypsin inhibitor.
6

A novel, stable, helical scaffold as an alternative binder - construction of phage display libraries

84%
Cyranka-Czaja A., Otlewski J.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
 Specific, high affinity binding macromolecules are of great importance for biomedical and biotechnological applications. The most popular classical antibody-based molecules have recently been challenged by alternative scaffolds with desirable biophysical properties. Phage display technology applied to such scaffolds allows generation of potent affinity reagents by in vitro selection. Here, we report identification and characterization of a novel helical polypeptide with advantageous biophysical properties as a template for construction of phage display libraries. A three-helix bundle structure, based on Measles virus phosphoprotein P shows a very favourable stability and solubility profile. We designed, constructed and characterized six different types of phage display libraries based on the proposed template. Their functional size of over 109 independent clones, balanced codon bias and decent display level are key parameters attesting to the quality and utility of the libraries. The new libraries are a promising tool for isolation of high affinity binders based on a small helical scaffold which could become a convenient alternative to antibodies.
7

The involvement of oxidative stress in determining the severity and progress of pathological processes in dystrophin-deficient muscles

84%
Niebroj-Dobosz I., Hausmanowa-Petrusewicz I.
Acta Biochimica Polonica
|
2005
|
tom 52
|
nr 2
In both forms of muscular dystrophy, the severe Duchenne’s muscular dystrophy (DMD) with lifespan shortened to about 20 years and the milder Becker dystrophy (BDM) with normal lifespan, the gene defect is located at chromosome locus Xp21. The location is the same in the experimental model of DMD in the mdx mice. As the result of the gene defect a protein called dystrophin is either not synthesized, or is produced in traces. Although the structure of this protein is rather well established there are still many controversies about the dystrophin function. The most accepted suggestion supposes that it stabilizes sarcolemma in the course of the contraction-relaxation cycle. Solving the problem of dystrophin function is a prerequisite for introduction of an effective therapy. Among the different factors which might be responsible for the appearance and progress of dystrophic changes in muscles there is an excessive action of oxidative stress. In this review data indicating the influence of oxidative stress on the severity of the pathologic processes in dystrophy are discussed. Several pieces of data indicating the action of oxidative damage to different macromolecules in DMD/BDM are presented. Special attention is devoted to the degree of oxidative damage to muscle proteins, the activity of neuronal nitric oxide synthase (nNOS) and their involvement in defining the severity of the dystrophic processes. It is indicated that the severity of the morbid process is related to the degree of oxidative damage to muscle proteins and the decrease of the nNOS activity in muscles. Estimation of the degree of the destructive action of oxidative stress in muscular dystrophy may be a useful marker facilitating introduction of an effective antioxidant therapy and regulation of nNOS activity.
8

Permeability studies on an in vitro model of the blood-brain barrier

84%
Deli M. A., Szabo C. A., Krizbai I., Abraham C. S., Joo F.
Folia Histochemica et Cytobiologica. Supplement
|
1997
|
tom 35
|
nr 2
9

Metabolic activation of adriamycin by NADPH-cytochrome P450 reductase; overview of its biological and biochemical effects

84%
Bartoszek A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 2
NADPH-cytochrome P450 reductase (P450 reductase) is one of the enzymes impli­cated in the metabolism of adriamycin, a very important clinically used antitumour drug. However, apart from the enzyme involvement, so far little was known about the chemical route and biochemical effects of this process. We demonstrated that the ap­plication of P450 reductase simultaneously with adriamycin to tumour cells in culture significantly increased cytotoxicity of the drug. Under tissue culture conditions, we noticed also that, in the presence of P450 reductase, adriamycin metabolite(s), dis­playing an altered spectrum within the visible light range were formed. This observa­tion was taken adavantage of to study the metabolism of adriamycin in cell-free sys­tems, using initially the enzyme isolated from rat liver and the recently obtained re­combinant human P450 reductase. The reductive conversion of the drug turned out to be a multi-stage process, which occurred only under aerobic conditions and was ac­companied by excessive NADPH consumption. Further research carried out with the aid of radical scavengers and radiolabelled adriamycin revealed that the enhance­ment of biological activity of adriamycin by P450 reductase stemmed from the forma­tion of alkylating metabolite(s) rather than from the promotion of redox cycling known to be induced in the presence of anthracyclines.
10

Dendrimers: properties and applications

84%
Klajnert B., Bryszewska M.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 1
Dendrimers are a new class of polymeric materials. They are highly branched, mono­disperse macromolecules. The structure of these materials has a great impact on their physical and chemical properties. As a result of their unique behaviour dendrimers are suitable for a wide range of biomedical and industrial applications. The paper gives a con­cise review of dendrimers' physico-chemical properties and their possible use in various areas of research, technology and treatment.
11

Makroczasteczki adhezyjne i ich rola w procesie nowotworowym

67%
Kozlowska K.
Acta Biologica et Medica
|
1992
|
tom 18
11-29
12

An update on some structural aspects of the mighty miniwall

67%
Markiewicz Z.
Polish Journal of Microbiology
|
2011
|
tom 60
|
nr 3
Peptidoglycan (PG), the mighty miniwall, is the main structural component of practically all bacterial cell envelopes and has been the subject of a wealth of research over the past 60 years, if only because its biosynthesis is the target of many antibiotics that have successfully been used in the treatment of bacterial infections. This review is mainly focused on the most recent achievements in research on the modification of PG glycan strands, which contribute to the resistance of bacteria to the host immune response to infection and to their own lytic enzymes, and on studies on the spatial organization of the macromolecule.
13

The influence of gamma-irradiation on denaturation of dry BAT collagen

67%
Kubisz L., Jaroszyk F.
Current Topics in Biophysics
|
1993
|
tom 17
|
nr 1-2
14

Markers of oxidative damage - applicability and limitations

67%
Jodynis-Liebert J.
Acta Poloniae Toxicologica
|
2002
|
tom 10
|
nr 2
15

Hydration of starch and protein seeds in early phase of germination

67%
Plenzler G. B., Napierala D. M., Baranowska H. M.
Żywność Nauka Technologia Jakość. Suplement
|
2002
|
tom 09
|
nr 4
Seed swelling in the first phase of imbibition involves mainly development of seed colloids. Chemical affinity of such colloids to water differs depending on the surface properties of the macromolecules. The biopolymer surface could perturb the dynamic and static state of water. For this reason the structure and composition of seeds, especially proteins, starch, and lipid content, can control the course of the swelling process. The study presents the microscopic and macroscopic parameters describing the swelling pea seeds and triticale grains. Differences in corresponding parameters were observed. Measurements of water uptake rate in both species showed higher water uptake in triticale grains compared to that in pea seeds in the first step of the process but lower in the subsequent phase. The results of pulse 1 H-NMR measurements have revealed two groups of water protons, each in a different magnetic environment responsible for a different relaxation rate. These two groups correspond to water molecules differing in mobility, such as free and bound water, respectively. The difference in results obtained for triticale and pea are related to size, different permeability of seed envelopes, different mobility of seed water and chemical content mainly determined by starch. Its structure and physicochemical properties are also very important.
16

Neutrophil myeloperoxidase: soldier and statesman

59%
Prokopowicz Z., Marcinkiewicz J., Katz D. R., Chaim B. M.
Archivum Immunologiae et Therapiae Experimentalis
|
2012
|
tom 60
|
nr 1
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