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Antibacterial activity of lysozyme modified by the membrane technique

100%

Cegielska-Radziejewska R., Lesnierowski G., Kijowski J.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

2003

tom 06

nr 2

The effectiveness of antibacterial action of lysozyme modified by the membrane technique (ultrafiltration and reverse osmosis) against selected strains of bacteria was determined. Its bacteriostatic activity was dependent on modification conditions. Among lysozyme preparations modified by ultrafiltration the highest bacteriostatic activity against selected strains of Proteus mirabilis, Pseudomonas fluorescens and Staphylococcus epidermidis bacteria was noted in the preparation containing 53.3% polymeric forms. The modification procedure facilitates the extension of antibacterial spectrum of lysozyme, particularly against Pseudomonas fluorescens and Proteus mirabilis Gram (-) bacteria.

Antibacterial activity of thermally modified lysozyme

100%

Lesnierowski G., Cegielska-Radziejewska R., Kijowski J.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

2001

tom 04

nr 2

Lysozyme (EC 3.2.1.17, mucopeptyde N-acetylmuramic-hydrolase) exhibits the ability to destroy cell walls of Gram-positive bacteria. The range of activity for this enzyme may be extended through modifications, as a result of which polymerized forms of lysozyme are obtained. It was found that lysozyme dimer exhibits bacteriostatic properties towards both Gram-positive and Gram-negative bacteria. The aim of the study was to determine the conditions of thermal modification of lysozyme and to assess the antibacterial action of this enzyme after modification. The dimer content in the modified lysozyme preparations depended on the pH and concentration of a given solution. It was found that modified lysozyme was characterized by higher antibacterial activity towards Micrococcus luteus and Escherichia coli in comparison to the unmodified form of the enzyme.

Ograniczanie wyników

2 Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

2 Cegielska-Radziejewska R.

2 Kijowski J.

2 Lesnierowski G.

1 2003

1 2001

Wyniki wyszukiwania

Antibacterial activity of lysozyme modified by the membrane technique

Antibacterial activity of thermally modified lysozyme