Wyniki wyszukiwania

1

Changes in pancreatic lysosomal enzymes activity as the potential factors leading to diabetic enteropathy

100%

Maciejewski R., Burski K., Baj J., Madej B., Burdan F., Dabrowski A., Brakowiecki F.

Journal of Physiology and Pharmacology

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2001

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tom 52

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nr 4,2

The aim of this study was to establish and quantify changes in the activities of total, free and bound fractions of pancreatic lipase, galactoso-6-sulphatase, ß-D-galactosidase and N-acetyl-ß-D-glucosaminidase in the course of alloxan-induced diabetes mellitus. Rabbits were divided into a control group and groups injected with alloxan on the 21 st , 42 nd , 90 th and the 180 th day, after which blood samples were taken and the rabbits sacrificed by decapitation. The pancreas was removed and the glucose level measured. Enzyme activities were assayed by spectrophotometric methods. The total activities of N-acetyl-ß-D-glucosaminidase and ß-D-galactosidase were the lowest on day 42 of the test, and the total activity of lipase was the highest at this point of time, as compared to the other periods of the study. We conclude that in the course of alloxan-induce diabetes activities of pancreatic lipase and sulphatase were increasing following the levels of glucose, whilst activities of ß-D-galactosidase and N-acetyl-ß-D-glucosaminidase were declining, being inversely correlated to the level of glucose and activities of the first two mentioned enzymes. Above alterations in activity of lysosomal pancreatic enzymes of alloxan induced diabetic rabbits may be responsible for some aspects of previously reported diabetic enteropathy and chronic complications, or may provide a mechanism for the pancreatic beta-cells to moderate their insulin content.

2

Effect of morphine on activity of five selected lysosomal enzymes and kidneys of mice

75%

Witek B., Ochwanowska E., Wrobel A., Kolataj A.

Animal Science Papers and Reports

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2011

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tom 29

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nr 2

The experiment was carried out on 90 Swiss male mice divided into 9 groups (n=10). Over 4, 10 and 14 days mice of three control groups (I-III) were injected with 250 μl 0.9% NaCl solution daily, and those from six experimental groups (A-F) with 250 μl 0.9% NaCl solution containing 20 or 30 mg morphine hydrochloride per kg body weight. The injections were given intramuscularly once a day between 9:00-10:00 a.m. for 4, 10 and 14 days. In the lysosomal fraction of the liver and kidney the activities of acid phosphatase, lysosomal esterase, β-glucuronidase, β-galactosidase, and β-N-acetyl-hexosaminidase were estimated. Morphine increased the activity of all examined enzymes except EL, which activity was statistically proven to decrease in liver and kidneys after 10 days morphine administration in both doses.

3

The influence of detergent SDS and fuel oil on the arylsulphatase and acid phosphatase activity in Gasterosteus aculeatus L.

75%

Drewa G., Chesy M., Chesy G.

Polskie Archiwum Hydrobiologii

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1999

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tom 46

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nr 1

4

Effect of bacitracin on Tetrahymena

75%

Kovacs P., Csaba G.

Acta Protozoologica

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1992

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tom 31

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nr 4

5

Rat sciatic nerve lysosomal enzyme activity as a test for noncholinergic neurotoxic effect of Bromphenvinphos

75%

Biel-Zielinska A., Gradowska-Olszewska I., Brzezinski J., Piesko T., Zajac A., Goldnik A.

Acta Poloniae Toxicologica

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1995

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tom 03

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nr 1

6

Activity of lysosomal enzymes in acute intoxication with organophosphorus insecticides

75%

Lukaszewicz-Hussain A., Moniuszko-Jakoniuk J.

Polish Journal of Environmental Studies

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1997

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tom 06

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nr 1

The aim of this paper was to examine the activity of lysosomal enzymes after intoxication with chlorfenvinvos and dichlorvos - phosphoorganic insecticides. Investigations were carried out on male Wistar rats weighing 180-230 g. Rats were divided into two groups: control and treated. Rats of treated group were receiving oil solution of chlorfenvinvos at doses of 0.5 LD50 or 0.1 LD5(), determinations were performed after 1 and 24 hours after treatment or dichlorvos in the dose equal to 0.5 LD50 estimations were performed after 1 h only. Rats of control group were given equivalent volume of oil. Activities of beta-glucuronidase and acid phosphatase in the blood serum and lysosomal fraction of rat liver homogenate were determined. It was found that the level of ChE inhibition and increase of BGR activity in the serum were dependant on chemical structure of used compound, dose and time after treatment. It was observed that increase of hydrolases activities in serum was accompanied by the decrease in its activity in the lysosomal fraction of liver homogenate.

7

The activation and inactivation of lysosomal and extralysosomal compartments - a simple general model

75%

Turski W. A., Zaslonka J., Szram S., Mussur M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

8

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity

63%

Minarowska A., Minarowski L., Karwowska A., Milewska A. J., Gacko M.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 1

9

Activity of some lysosomal enzymes in adrenal cortex during experimental alloxan-induced diabetes mellitus in rabbits

63%

Grzebalska A. M., Cendrowska-Pinkosz M., Luszczewska-Sierakowska I., Burdan F., Krauze M.

Medycyna Weterynaryjna

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2014

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tom 70

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nr 04

The aim of the study was the evaluation of changes in the adrenal cortex lysosomal enzymes activity during experimental alloxan-induced diabetes mellitus in rabbits. We checked the activity of acid phosphatase, β-D-galactosidase, N-acetyl-β-D-glucosaminidase (NAGL) and lipase. The study was performed on 124 rabbits divided into five groups: one control and four experimental. Diabetes mellitus was induced by a single injection of 10% alloxan solution into the auricular vein in a dose of 10 mg per kg body weight. Animals from experimental groups were killed in the 21st, 42nd, 90th and 180th days of the study. Adrenal glands were removed. Enzymes activity was assayed by spectrophotometric methods. Changes in free and bound fractions of examined lysosomal enzymes activity were noticed already in the 21st day of diabetes. The most escalated changes were observed in the 42nd day of the study. Performed statistical variance analysis demonstrated statistically highly significant differences for activity of both fractions of NAGL and lipase, as well as for free fraction activity of acid phosphatase and β-D-galactosidase. The obtained data confirmed the influence of diabetes mellitus on changes in the activity of examined lysosomal enzymes in the adrenal cortex.

10

Stress-induced enhancement of activity of lymphocyte lysosomal enzymes in pigs of different stress-susceptibility

63%

Stojek W., Borman A., Glac W., Baracz-Jozwik B., Witek B., Kamyczek M., Tokarski J.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 8

61-72

To evaluate a possible mechanism of stress-induced lymphopenic effect we assessed the activity of lymphocyte lysosomal enzymes (LE) under immobilization. The effects of immobilization stress on LE (AP, acid phosphatase, cathepsin D and L, ß-N-acetyl-glucosamidase) activity in lymphocytes, number of lymphocytes and plasma cortisol (COR) level in the peripheral blood were examined in the cross-bred Pietrain pigs showing genotypic (presence or lack of RyR1 gene mutation) and phenotypic (reactivity to halothane) differences. It was found that immobilization stress evoked an increase in LE which was concomitant with lymphopenia and a rise of COR level. The most pronounced enhancement of LE, which may reflect a tendency to lymphocyte cytolysis, was found in the recessive homozygotes RyR1 (nn) phenotypically defined as stress/halothane susceptible as well as in the heterozygotes RyR1 (Nn) included in the group of stress/halothane resistant. Despite this individual variability the stress-induced increase in LE activity was present in all the animals. It seems that a possibility of destruction (lysis) of lymphocyte cells should not be excluded as one of the causes of stress lymphopenia.

11

Cytochemical studies on the secretory activity of guinea pig heterophols during sensitization

63%

Godlewski A.

Folia Histochemica et Cytobiologica

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1985

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tom 23

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nr 1-2

12

Missorting of cathepsin B into the secretory compartment of CI-MPR/IGFII-deficient mice does not induce spontaneous trypsinogen activation but leads to enhanced trypsin activity during experimental pancreatitis - without affecting disease severity

63%

Meister T., Niehues R., Hahn D., Domschke W., Sendler M., Lerch M. M., Schnekenburger J.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 5

13

The activity and immunoexpression of cathepsin D in rat male reproductive organs

63%

Burdan F., Szumilo J., Dudka J., Dabrowski A., Maciejewski R., Wojtowicz Z.

Folia Morphologica

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2006

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tom 65

|

nr 2

111-115

Cathepsin D is a cysteine endopeptidase that belongs to the lysosomal enzyme family. The aim of the study was to evaluate the enzyme immunoexpression and activity in selected male genital organs in mature Wistar rats. The activity of cathepsin D was measured spectrophotometrically in homogenates of the testis, epididymis, seminal vesicle and prostate. Immunohistochemical staining was also performed in the ductus deferens. Enzyme activity was found in the following sequence: testis>epididymis>dorsal prostatic lobe>seminal vesicle>lateral prostatic lobe>ventral prostatic lobe. Although there were differences in enzyme activity between various organs of the male reproductive system, cathepsin D immunoreactivity was seen exclusively in the Sertoli and Leydig cells in the testis.

14

Activity of lysosomal enzymes in the liver and kidneys of mice after morphine administration

63%

Witek B., Ochwanowska E., Wrobel A., Kolataj A.

Animal Science Papers and Reports

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2010

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tom 28

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nr 2

Used were ninety Swiss mice males divided into 9 groups (n=10). Three control groups [I, II, III] were injected with 0.9% NaCl solution, while six experimental groups (A, B, C, D, E, F) with the morphine hydrochloride. Mice from groups A, C and E were injected with a dose of 20 and those from B, D and F with a dose of 30 mg morphine per kg body weight. In each group, both solutions were administered intramuscularly once a day from 9:00 to10:00 a.m. for 4, 10 and 14 days.In the lysosomal fraction of the liver and kidney the activities of cathepsin D, cathepsin L, alanine aminopeptidase, leucine aminopeptidase, lysosomal lipase, and β-glucosidase were estimated.Morphine led to the increased activity of all examined enzymes except lysosomal lipase, the activity of which dropped in both organs examined.

15

Effects of a gold salt and its intracellular distribution in Tetrahymena

63%

Nilsson J. R.

Acta Protozoologica

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1993

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tom 32

|

nr 3

16

Effect of aluminum on the activity of model lysosomal hydrolases and the ultrastructure of mouse hepatocyte

63%

Krol T., Lach H., Wieczorek A., Lysek-Gladysinska M., Staszczyk K., Mycinska M., Gren A.

Acta Biologica Cracoviensia. Series Zoologia

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2004

|

tom 46

17

Effects of paclitaxel on lysosomal enzyme activity and ultrastructure of mouse liver cells

63%

Krol T.

Acta Biologica Cracoviensia. Series Zoologia

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2002

|

tom 44

18

Effect of glucose, glutathione and ethanol on the activity of some lysosomal enzymes in quail

63%

Witek B., Kolataj A., Jezewska G., Witkowski A.

Acta Biologica Cracoviensia. Series Zoologia

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2000

|

tom 42

19

Lysosomal enzymes in urine for the assessment of renal function in workers exposed to gasoline

63%

Marchewka Z., Stoklosa A., Dlugosz A.

Acta Toxicologica

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2005

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tom 13

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nr 2

20

Effect of antioxidants on lysosomal enzyme activity in mouse liver, kidney and muscle, I. Effect of vitamin C

63%

Kolataj A., Konecka A. M., Sliwa-Jozwik A., Jozwik A.

Acta Biologica Cracoviensia. Series Zoologia

|

2003

|

tom 45

Ograniczanie wyników

Changes in pancreatic lysosomal enzymes activity as the potential factors leading to diabetic enteropathy

Effect of morphine on activity of five selected lysosomal enzymes and kidneys of mice

The influence of detergent SDS and fuel oil on the arylsulphatase and acid phosphatase activity in Gasterosteus aculeatus L.

Effect of bacitracin on Tetrahymena

Rat sciatic nerve lysosomal enzyme activity as a test for noncholinergic neurotoxic effect of Bromphenvinphos

Activity of lysosomal enzymes in acute intoxication with organophosphorus insecticides

The activation and inactivation of lysosomal and extralysosomal compartments - a simple general model

Role of cathepsin A and cathepsin C in the regulation of glycosidase activity

Activity of some lysosomal enzymes in adrenal cortex during experimental alloxan-induced diabetes mellitus in rabbits

Stress-induced enhancement of activity of lymphocyte lysosomal enzymes in pigs of different stress-susceptibility

Cytochemical studies on the secretory activity of guinea pig heterophols during sensitization

Missorting of cathepsin B into the secretory compartment of CI-MPR/IGFII-deficient mice does not induce spontaneous trypsinogen activation but leads to enhanced trypsin activity during experimental pancreatitis - without affecting disease severity

The activity and immunoexpression of cathepsin D in rat male reproductive organs

Activity of lysosomal enzymes in the liver and kidneys of mice after morphine administration

Effects of a gold salt and its intracellular distribution in Tetrahymena

Effect of aluminum on the activity of model lysosomal hydrolases and the ultrastructure of mouse hepatocyte

Effects of paclitaxel on lysosomal enzyme activity and ultrastructure of mouse liver cells

Effect of glucose, glutathione and ethanol on the activity of some lysosomal enzymes in quail

Lysosomal enzymes in urine for the assessment of renal function in workers exposed to gasoline

Effect of antioxidants on lysosomal enzyme activity in mouse liver, kidney and muscle, I. Effect of vitamin C