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Polymerase chain reaction for the differentiation of Marek's disease virus strains

100%

Kozdrun W., Samorek-Salamonowicz E., Czekaj H.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

The aim of this study was an attempt to apply PCR for diagnosis of Marek's disease. Eighteen field Marek's disease virus strains and 4 standard strains: virulent HPRS₁₆ strain and attenuated CVI 988 strain (serotype 1 - MDV-1), apathogenic SB 1strain (serotype 2 - MDV-2) and non - pathogenic turkeys HVT FC 126 strain (serotype 3 MDV-3) were used. Chicken anaemia virus (CAV) and infectious laryngotracheitis virus (ILTV) were used as control of PCR. The virus strains were cultured in CEF and total DNA was isolated by A@A Biotechnology kit. Three pairs of primers were used: for gene A of serotype 1, for 132 bp sequence of serotype 1 and for gene A of serotype 3. PCR was carried out under the following conditions: 94°C - 1 min (denaturation), 55°C - 30 s (annealing), 72°C -30 s (elongation). PCR products were analysed by electrophoresis in 2% agarose gel at 100 V/h. The PCR product of 314 bp was obtained from all field strains and HPRS₁₆ standard after the primers for gene A of serotype 1 were used. After the primers for 132 bp sequence of serotype 1 were used, 434 bp fragment for 19 DNA samples derivied from field strains and standard HPRS₁₆ strain and 1033 bp fragment from attenuated CVI 988 strain were obtained. Application of primers for gene A serotype 3 allowed the detection of 436 bp product only from non-pathogenic HVT FC 126 strain (serotype 3). These results indicated that the PCR technique can be used for the differentiation of MDV strains.

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Histomorphometric study of megakaryocytes in bone marrow in selected myeloproliferative and lymphoproliferative diseases

100%

Lemancewicz D., Dzieciol J., Kloczko J., Litwiejko-Pietrynczak E., Boguslowicz W.

Folia Morphologica

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2003

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tom 62

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nr 1

The aim of the study was an estimation of the histomorphometry of megakaryocytes (MK) in bone marrow in selected myeloproliferative and lymphoproliferative diseases. Bone marrow specimens were obtained by trephine biopsies from 41 patients with polycythaemia rubra vera (PV), idiopathic myelofibrosis (MF), chronic lymphocytic leukaemia (CLL), hairy cell leukaemia (HCL) and diffuse large B-cell lymphoma (L). Morphometric evaluation was performed using a standard program set MicroImage (OLYMPUS). The greatest number of typical nucleated MK, “naked” nuclei, anucleated cytoplasmic fragments and the largest area were found in PV. The circular deviation factor of MK and their nuclei increased in all cases. The greatest number of clusters was observed in PV and HCL. A significant increase in the number of dysplastic and “naked” nuclei of MK was noted in all selected haematological diseases. The presence of neoplastic cells in bone marrow increased the morphological changes in MK. Quantitative and morphometrical significant differentiation of MK in separate microscopic field in the same slides confirms the necessity of performing trephine biopsies in each patient with haematological disorders.

3

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay

80%

Bicka L., Kuzmak J., Kozaczynska B., Plucienniczak A., Skorupska A.

Acta Biochimica Polonica

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2001

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tom 48

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nr 1

The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.

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Effect of mitogens on the in vitro stimulation of lymphocytes from normal and BLV-infected cattle

80%

Stec J., Grundboeck-Jusko J., Szczotka M., Reichert M., Rulka J., Kozaczynska B.

Bulletin of the Veterinary Institute in Puławy

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1993

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tom 37

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nr 2

Ograniczanie wyników

2 Bulletin of the Veterinary Institute in Puławy

1 Acta Biochimica Polonica

1 Folia Morphologica

2 Kozaczynska B.

1 Bicka L.

1 Boguslowicz W.

1 Czekaj H.

1 Dzieciol J.

1 Grundboeck-Jusko J.

1 Kloczko J.

1 Kozdrun W.

1 Kuzmak J.

1 Lemancewicz D.

1 Litwiejko-Pietrynczak E.

1 Plucienniczak A.

1 Reichert M.

1 Rulka J.

1 Samorek-Salamonowicz E.

1 Skorupska A.

1 Stec J.

1 Szczotka M.

1 2003

2 2001

1 1993

Polymerase chain reaction for the differentiation of Marek's disease virus strains

Histomorphometric study of megakaryocytes in bone marrow in selected myeloproliferative and lymphoproliferative diseases

Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay

Effect of mitogens on the in vitro stimulation of lymphocytes from normal and BLV-infected cattle