Wyniki wyszukiwania

1

Lymphadenomegaly in dogs - cytological study

100%

Sapierzynski R., Micun J.

Polish Journal of Veterinary Sciences

|

2009

|

tom 12

|

nr 2

263-268

Lymphadenomegaly is the enlargement of the lymph node/nodes, due to various nonneoplastic or neoplastic processes, observed mainly in dogs, rarely in other animal species. The first step of a diagnostic procedure in cases of lymph nodes enlargement, apart from physical examination and morphological blood analysis, should be a cytological examination of an enlarged lymph node/nodes. The aim of the present study was to determine the character and the cause of the lymph node/nodes enlargement in dogs and to evaluate the usefulness of fine-needle cytology in a diagnosis of lymphadenomegaly. The study was performed on dogs submitted to consultation in the Small Animal Clinic of Department of Clinical Sciences, Warsaw University of Life Sciences, due to generalized or local lymphadenomegaly, and in which fine-needle biopsy was made. A signalement, general state of dog, the presence and duration of clinical signs, the character (generalized vs. localized) and intensity (mild, moderate or severe) of lympadenomegaly were recorded during physical examination. The average age of dogs examined was 8 years, 64 were males and 36 were females of various breeds; however the majority of them were greater than 25 kg in weight. The cytological diagnosis including most often lymphoma and reactive hyperplasia, more seldom metastatic neoplasm and lymphadenitis were recognized, some of the samples were considered nondiagnostic/nondefined. On the basis of the present results it can be concluded that cytological examination of good quality samples allows to obtain final diagnosis in over 90% of lymphadenomegaly. Higher incidence of canine lymphomas in males is suggested.

2

Monocytes in children with leukemias and lymphomas - down-regulation of HLA and costimulatory molecules

100%

Luczynski W., Stasiak-Barmuta A., Krawczuk-Rybak M., Szymanski M., Malinowska I., Mitura-Lesiuk M.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 4

The aim of the study was to evaluate the function of monocytes in children with leukemias and lymphomas based on the expression of critical costimulatory, activatory and adhesion molecules (CD80, CD86, HLA-DR and CD54 = ICAM-1), estimated with tricolor flow cytometry. In comparison to the control group we found a lower percentage of monocytes with costimulatory molecules (CD80 before and CD86 after lipopolysaccharide stimulation) at the time of diagnosis and of monocytes with HLA-DR molecules after remission induction. We also noted a lower percentage of monocytes with HLA-DR expression in the group with severe or therapy resistant infections. The results of our investigation suggest some defect in costimulation and antigen presentation in lymphoproliferative diseases in children.

3

Usefulness od real-time PCR in long-term follow-up of follicular lymphoma patients

100%

Tysarowski A., Fabisiewicz A., Paszkiewicz-Kozik E., Kulik J., Walewski J., Siedlecki J. A.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 1

The aim of this study was to evaluate the usefulness of quantitative real-time PCR (RQ-PCR) for the monitoring of molecular remission in follicular lymphoma (FL) patients during long-term follow-up. RQ-PCR by the use of TaqMan® detection system is a sensitive tool to monitor minimal residual disease (MRD) in FL through amplification of the t(14;18) fusion gene during and post-therapy. In most cases the breakpoint region occurs within the major breakpoint region (MBR). Among 75 patients diagnosed with FL, cells harboring the fusion gene BCL2/JH were found in peripheral blood of 31 patients (41%). We further monitored 30 of these patients in a period varying from 6 months to 5 years by RQ-PCR. In our study the level indicating the possibility of the presence of MRD was established at more than five t(14;18)-positive cells in the background of 83000 normal cells. The results of this work also confirmed that the presence of MRD detected by RQ-PCR is an indication for careful observation of patients because of a higher risk of disease recurrence.

4

Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas

100%

Luczynski W., Kovalchuk O., Krawczuk-Rybak M., Mitura-Lesiuk M., Malinowska I., Chyczewski L., Kowalczyk J., Matysiak M.

Folia Histochemica et Cytobiologica

|

2005

|

tom 43

|

nr 3

5

Immunocytochemical and cytomorphometric diagnostics of malignant lymphomas in dogs

100%

Dzimira S.

Bulletin of the Veterinary Institute in Puławy

|

2007

|

tom 51

|

nr 1

Monoclonal antibodies are used to determine whether tumour cells of malignant lymphomas in dogs belong to B or T lymphocytes, to define the proliferative potential of the cells (Ki-67 protein, PCNA antigen, AgNORs) and to demonstrate effects of potential mutations of the p53 gene. Among 76 examined cases, 58 tumours (76.32%) were lymphomas of lymphocytes B; the remaining 18 (23.68%) cases were qualified as originating from lymphocytes T. Lymphomas with a high grade of malignancy prevailed (54 cases, 71.06%), and also in this group of lymphomas of lymphocytes B prevailed (48 cases, 88.89%). The remaining 22 cases (28.95%) represented less malignant lymphomas, including 12 cases of lymphocyte T lymphomas (54.54%). In the group of highly malignant lymphomas, high values of Ki- 67 and PCNA indices were demonstrated. Positive reactions of low intensity with anti-p53 protein were obtained in 31 cases (57.4%) of highly malignant and in 9 cases (40.9%) of less malignant lymphomas. In dogs, most frequently malignant centroblastic B receptor lymphomas are encountered. The extent of malignancy is determined by young forms and their proliferation exponents are Ki-67 and PCNA proteins.

6

DNA repair in drug resistance induced by fusion tyrosine kinases

100%

Blasiak J.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

7

Association of EBV infection with lymphomas

100%

Trzcinska A., Litwinska B.

Acta Microbiologica Polonica

|

2003

|

tom 52

|

nr 2

8

Cytometric evaluation of transferrin receptor 1 (CD71) in childhood acute lymphoblastic leukemia

84%

Ploszynska A., Ruckemann-Dziurdzinska K., Jozwik A., Mikosik A., Lisowska K., Balcerska A., Witkowski J. M.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 2

9

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Cytopathology of canine lymphomas (100 cases)

84%

Sapierzynski R., Micun J., Jagielski D., Jurka P.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 4

Malignant lymphoma is one of the most common malignant tumours occurring in dogs. Fine needle aspiration biopsy (FNAB) is an excellent, specific diagnostic procedure used to assess pathological processes in lymph nodes. The aim of the present study was to conduct a cytopathological analysis of lymphoma in dogs and to analyse some epidemic aspects of occurrence of lymphoma in 100 dogs using Giemsa stained slides. Samples were obtained by fine needle aspiration biopsy, fine needle non-aspiration biopsy, lymph node impression smears and by examination of body cavity effusions. The determination of the type and subtype of tumour was made on the basis of the updated Kiel classification adopted for dogs. Based on cytopathological analysis, the lymphoma was diagnosed in 100 dogs: 44 were female and 56 male. The animals’ age ranged from 1.5 year to 15 years (median: 7.5 years), the animals were of different breeds (72% of all dogs belonged to 28 different breeds) and crossbreeds (28%). In 29% of dogs the regional or general lymphadenomegaly was the only clinical sign observed, in remaining cases (71%) at least one abnormality connected to lymphoma was found. Among all diagnosed lymphomas, high-grade lymphomas were more prevalent (86% of all cases) than low-grade lymphomas (14% of all cases). The possibility of boxers having a predisposition to T cell lymphoma development could be also suspected.

10

Canine oral cavity T-cell lymphoma - histopathological and immunohistochemical study

84%

Pazdzior-Czapula K., Otrocka-Domagala I., Gesek M., Mikiewicz M.

Medycyna Weterynaryjna

|

2018

|

tom 74

|

nr 01

This study presents a case of an unusually located canine T-cell lymphoma. A 5-year-old female dachshund was presented with a tumour located in the buccal mucosa. The tumour was excised, fixed, processed routinely for histopathology and stained. Microscopically, a dense infiltration of round cells with scant cytoplasm, large nuclei and numerous mitotic figures was detected within the mucosa. The tumour was diagnosed as a roundcell tumour. Subsequently, additional tumours developed in the mandibular and hock joint areas. The primary tumour was stained immunohistochemically using an antibody panel (CD3, MHCII, mast cell tryptase, CD18, CD79a). The tumour cells showed variable cytoplasmic expression of CD3, moderate-to-strong cytoplasmic or membranous expression of MHCII, and they were mast cell tryptase, CD18 and CD79a negative. The final diagnosis was T-cell lymphoma. The dog passed away within the next two months. This study revealed, that immunohistochemistry is necessary to diagnose canine oral cavity round cell tumours.

11

Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas

84%

Sokolowska J., Micun J., Zabielska K., Malicka E., Lechowski R.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 4

Nineteen canine lymphomas were included in this study. Tumors were classified according to the updated Kiel classification adapted for canine lymphomas by Fournel-Fleury et al. Immunoglobulin light chains (κ and λ) and IgM and IgG expression were determined by immunohistochemical method. In all examined cases neoplastic cells were positive for one of the immunoglobulin light chains. Expression of λ light chains and κ light chains was observed in 18/19 and 1/19 tumors, respectively. In the majority of neoplastic cells in each examined specimen this reaction had a membranous pattern (sκ/sλ). In all examined cases the presence of immunoglobulin light chains was also observed in the cytoplasm of some neoplastic cells (cκ/cλ). These cells were usally rare and never constituted a dominant population. The expression of immunoglobulin was found in 13/19 cases. Most lymphomas were sIgM positive (11/13 cases). In one case expression of IgG was found, and in another lymphoma two populations of neoplastic cells with different expression of examined immunoglobulins (cells with IgM+ and IgG+ phenotypes) were observed. The reaction also had a membranous pattern. The cells containing cytoplasmic immunoglobulins were rare, and in most cases were of the same type as the surface immunoglobulins. Our study has confirmed that canine lymphomas are a monoclonal proliferation of B-cells usually expressing immunoglobulin λ light chains and that the vast majority of tumors deriving from B-cells express IgM. Our study also indicates a possibility of occurence of biclonal lymphomas in canine species.

12

The cyclooxygenase-2/prostaglandin E2 pathway and its role in the pathogenesis of human and dog hematological malignancies

84%

Zmigrodzka M., Rzepecka A., Krzyzowska M., Witkowska-Pilaszewicz O., Cywinska A., Winnicka A.

Journal of Physiology and Pharmacology

|

2018

|

tom 69

|

nr 5

13

Soluble angiogenesis markers in gastric tumor patients

84%

Mysliwiec P., Pawlak K., Bandurski R., Kedra B.

Folia Histochemica et Cytobiologica

|

2009

|

tom 47

|

nr 1

81-86

14

STAT3 knockdown by siRNA induces apoptosis in human cutaneous T-cell lymphoma line Hut78 via downregulation of Bcl-xL

84%

Verma N. K., Davies A. M., Long A., Kelleher D., Volkov Y.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 2

Cutaneous T-cell lymphomas (CTCLs) are non-Hodgkin’s lymphomas resulting from clonal expansion and localization of malignant T-lymphocytes to the skin. CTCL cells have defective apoptosis. Signal transducers and activators of transcription (STAT) are a family of transcription factors known to play important roles in the development and progression of several human cancers by promoting cell proliferation and protecting against apoptosis. In this study, we investigated the specific role of STAT3, a major component of the STAT family, in growth and survival of human CTCL cell line Hut78. Western immunoblot analysis showed elevated expression of STAT3 and phospho-STAT3(Y705) in human CTCL cells as compared to freshly isolated peripheral blood lymphocytes (PBLs). Specific knockdown of STAT3 expression in Hut78 cells by RNA interference induced morphological and biochemical changes indicating apoptotic cell death. Moreover, STAT3 inhibition downregulated the expression of Bcl2 family of anti-apoptotic gene Bcl-xL. These observations suggest that STAT3 is required for the survival of CTCL cells and strongly indicate that targeting STAT3 using siRNA techniques may serve a novel therapeutic strategy for the treatment of CTCL.

15

Systemic administration of 2-chloro-2'-deoxyadenosine [2-CdA] in patients with systemic scleroderma

84%

Majewski S., Skopinska M., Blaszczyk M., Ryba M., Grieb P., Chorzelski T., Jablonska S.

Archivum Immunologiae et Therapiae Experimentalis

|

1994

|

tom 42

|

nr 1

16

2-chloro-2'-deoxyadenosine [2-CdA] treatment in a patient with Waldenstrom's macroglobulinaemia complicated by severe neutrocytopenia

84%

Zaucha J. M., Ciesielski D., Grieb P., Hellmann A.

Archivum Immunologiae et Therapiae Experimentalis

|

1994

|

tom 42

|

nr 1

17

Host immune response in B-cell lymphomas: friend or foe?

84%

Juszczynski P., Nowak J., Warzocha K.

Archivum Immunologiae et Therapiae Experimentalis

|

2008

|

tom 56

|

nr 4

18

Reactivity of monoclonal antibodies OK-CLL [anti-CD5] with peripheral blood cells of patients with B cell lymphoproliferative disorders

84%

Milosevic D., Marinkovic M., Jelic S., Colovic M., Spuzic I.

Archivum Immunologiae et Therapiae Experimentalis

|

1995

|

tom 43

|

nr 1

19

DNA-protection cross-linking induced by nitracrine in two strains of mouse lymphoma L5178Y cells

84%

Ciesielska E., Walicka M., Pastwa E., Szmigiero L.

Acta Biochimica Polonica

|

1992

|

tom 39

|

nr 1

20

Cytotoxic effect of nitric oxide on human hematological malignant cells

84%

Tsumori M., Tanaka J., Koshimura K., Kawaguchi M., Murakami Y., Kato Y.

Acta Biochimica Polonica

|

2002

|

tom 49

|

nr 1

We investigated the cytotoxic effect of nitric oxide (NO) on primary culture of human hematological malignant cells. Sodium nitroprusside (SNP), an NO donor, had cytotoxic effects on the cells of some patients with malignant lymphoma (ML), acute myelocytic leukemia (AML) or chronic myelomonocytic leukemia (CMMoL), but not with multiple myeloma. Cultured cells from the ML patient remained sensitive to SNP after the cells became resistant to anti-cancer drugs. In contrast, the cells from the patients with AML and CMMoL became resistant to SNP while anti-cancer drugs remained effective. In samples of the cells of the patients with ML and AML, the number of CD3 positive lymphoma cell was decreased by SNP and the number of CD33 negative cells and normal B lymphocytes (CD19 positive cells) were increased. In the cells of the patient with ML, apoptosis was induced by SNP. SNP had no effect on lymphocytes of healthy volunteers. These results suggest that SNP had an anti-tumor effect on human hematological malignant cells.

Ograniczanie wyników

Lymphadenomegaly in dogs - cytological study

Monocytes in children with leukemias and lymphomas - down-regulation of HLA and costimulatory molecules

Usefulness od real-time PCR in long-term follow-up of follicular lymphoma patients

Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas

Immunocytochemical and cytomorphometric diagnostics of malignant lymphomas in dogs

DNA repair in drug resistance induced by fusion tyrosine kinases

Association of EBV infection with lymphomas

Cytometric evaluation of transferrin receptor 1 (CD71) in childhood acute lymphoblastic leukemia

Cytopathology of canine lymphomas (100 cases)

Canine oral cavity T-cell lymphoma - histopathological and immunohistochemical study

Immunohistochemical study of expression of immunoglobulins in canine B-cell lymphomas

The cyclooxygenase-2/prostaglandin E2 pathway and its role in the pathogenesis of human and dog hematological malignancies

Soluble angiogenesis markers in gastric tumor patients

STAT3 knockdown by siRNA induces apoptosis in human cutaneous T-cell lymphoma line Hut78 via downregulation of Bcl-xL

Systemic administration of 2-chloro-2'-deoxyadenosine [2-CdA] in patients with systemic scleroderma

2-chloro-2'-deoxyadenosine [2-CdA] treatment in a patient with Waldenstrom's macroglobulinaemia complicated by severe neutrocytopenia