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The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X-100

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Panasiewicz M., Domek H., Hoser G., Fedoryszak N., Kawalec M., Pacuszka T.

Cellular and Molecular Biology Letters

2009

tom 14

nr 2

175-189

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Domek H.

1 Fedoryszak N.

1 Hoser G.

1 Kawalec M.

1 Pacuszka T.

1 Panasiewicz M.

1 2009

Wyniki wyszukiwania

The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X-100