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Our study evaluated the sensitivity and repeatability of nested PCR-based assays for directly detecting L. monocytogenes in artificially contaminated human serum, pasteurized milk and physiological saline samples. The detection of the hlyA (267bp) and iap (371bp) gene fragments was compared. The logistic regression (logit model) was used to evaluate the probability of detection of L. monocytogenes at various contamination levels and to calculate the number of test repetitions required to reach necessary detection limits (e.g. 50%, 80%, 90%, and 95%). The reliable limit of detection for both genetic markers, ensuring ≥95% probability of detection, was established at 102 CFU/100μL.
The observational study was carried out in a population of Polish breeding goats in 2007 to determine the prevalence of fetal loss and identify risk factors contributing to its occurrence. The multivariate model allowing to predict the risk of the occurrence of fetal loss in a herd in a study population was developed. Data on the occurrence of fetal loss, as well as of 28 hypothesized risk factors were collected from goat owners using standardized questionnaire during face-to-face reviews on farms. Moreover, data on the herd-level seroprevalence of four abortifacient infections – Chlamydophila abortus, Leptospira spp., BVDV-1 and Neospora caninum – were included in the final analysis. Fetal loss was reported as occurring often in 12 of 49 goat herds (24.5%). The relationship between the hypothesized risk factors and the occurrence of fetal loss was verified in the multivariate logistic regression (α=0.05). Final analysis yielded four risk factors: regular veterinary supervision at least twice a year (OR 0.188; CI 95% 0.054 – 0.656), frequent occurrence of injuries and fractures (OR 3.172; CI 95% 1.081 – 9.310), frequent occurrence of respiratory signs in adult goats (OR 4.848; CI 95% 1.353 – 17.377) and presence of antibodies to C. abortus in a herd (OR 58.116; CI 95% 1.369 – 2466.438). The accuracy of the multivariate model was analyzed using receiver operating characteristic (ROC) curve technique. Area under the curve was 0.895 (CI 95% 0.801-0.981). For optimal cut-off value of 0.20-0.35 the multivariate model had sensitivity of 75.00% and specificity of 89.19% in predicting fetal loss in a herd.
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This paper proposes a method to unify the defi nition of the main pollen season based on statistical analysis. For this, an aerobiological study was carried out in Porto region (Portugal), from 2003-2005 using a 7-day Hirst-type volumetric spore trap. To defi ne the main pollen season, a non-linear logistic regression model was fi tted to the values of the accumulated sum of the daily airborne pollen concentration from several allergological species. An important feature of this method is that the main pollen season will be characterized by the model parameters calculated. These parameters are identifi able aspects of the fl owering phenology, and determine not only the beginning and end of the main pollen season, but are also infl uenced by the meteorological conditions. The results obtained with the proposed methodology were also compared with two of the most used percentage methods. The logistic model fi tted well with the sum of accumulated pollen. The explained variance was always higher than 97%, and the exponential part of the predicted curve was well adjusted to the time when higher atmospheric pollen concentration was sampled. The comparison between the different methods tested showed large divergence in the duration and end dates of the main pollen season of the studied species.
The predictive modeling of plant species distribution has wide applications in vegetation studies. This study attempts to assess three modeling approaches to predict the plant distribution in the dry (precipitation 128–275 mm) mountainous (altitude 1129–2260 m a.s.l.) scrub vegetation on the example of the rangelands of northeastern Semnan, Iran. The vegetation of the study area belongs to the communities of Artemisia, Astralagus, Eurotia and other scrub species. The main objective of this study is to compare the predictive ability of three habitat models, and to find the most effective environmental factors for predicting the plant species occurrence. The Canonical Correspondence Analysis (CCA), Logistic Regression (LR), and Artificial Neural Network (ANN) models were chosen to model the spatial distribution pattern of vegetation communities. Plant density and cover, soil texture, available moisture, pH, electrical conductivity (EC), organic matter, lime, gravel and gypsum contents and topography (elevation, slope and aspect) are those variables that have been sampled using the randomized systematic method. Within each vegetation type, the samples were collected using 15 quadrates placed at an interval of 50 m along three 750 m transects. As a necessary step, the maps of all factors affecting the predictive capability of the models were generated. The results showed that the predictive models using the LR and ANN methods are more suitable to predict the distribution of individual species. In opposite, the CCA method is more suitable to predict the distribution of the all studied species together. Using the finalized models, maps of individual species (for different species) or for all the species were generated in the GIS environment. To evaluate the predictive ability of the models, the accuracy of the predicted maps was compared against real-world vegetation maps using the Kappa statistic. The Kappa (κ) statistic was also used to evaluate the adequacy of vegetation mapping. The comparison between the vegetation cover of a map generated using the CCA application and its corresponding actual map showed a good agreement (i.e. κ= 0.58). The results also revealed that maps generated using the LR and ANN models for Astragalus spp., Halocnemum strobilaceum, Zygophyllum eurypterum and Seidlitzia rosmarinus species have a high accordance with their corresponding actual maps of the study area. Due to the high level of adaptability of Artemisia sieberi, allowing this specie to grow in most parts of the study area with relatively different habitat conditions, a predictive model for this species could not be fixed. In such cases, a set of predictive models may be used to formulate the environment-vegetation relationship. Finally, the predictive ability of the LR and ANN models for mapping Astragalus spp. was determined as κ = 0.86 and κ = 0.91 respectively, implying a very good agreement between predictions and observations. It is concluded that the combination of mod- elling of the local species distribution constitutes a promising future research area, which has the potentiality to enhance assessments and conservation planning of vegetation (like rangelands) based on predictive species models.
Elephants were confined to Mengyang Protected Area in China and their distribution range had reduced greatly compared to past records. A preliminary study of habitat selection by Asian elephantsElephas maximus Linnaeus, 1758 and their distribution was conducted in Mengyang Protected Area and its surrounds using site visits and transect surveys from July 2003 to December 2006. Although no variable significantly influenced their habitat selection, elephants still showed preference for altitudes between 900 and 1200 m, gradients <30°, and orientations to the south-east, south and south-west. Human activities, including habitat transformation and degradation, disturbance by large infrastructure and poaching were considered to be the main factors inducing elephant distribution changes.
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