Wyniki wyszukiwania

1

High density lipoprotein and low density lipoprotein-mediated changes in cultured human skin fibroblasts

100%

Jozwiak Z., Szosland D., Rozga B.

Cellular and Molecular Biology Letters

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1996

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tom 01

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nr 2

We investigated the effect of native and modified high density lipoprotein (HDL) and low density lipoprotein (LDL) on the viability and the membrane fluidity of normal and diabetic fibroblasts. Under experimental conditions, HDL did not affect the cultured normal cells. Incubation of HDL with diabetic fibroblasts resulted in a negligible decrease in the cell viability and induced an increase in the lipid fluidity at surface of the cell membranes. LDL at the higher concentration reduced the viability both normal and diabetic fibroblasts and also enhanced the fluidity of membrane lipids. The above changes in the fluidity of fibroblast membranes were observed mainly in the presence of native and oxidized lipoproteins and are connected with the differences in the structure of cell membranes and nature of the cytotoxicity of LDL and HDL.

2

Ultrastructural studies of the liver during experimental hypercholesterolaemia

100%

Fortak A., Fortak W.

Folia Histochemica et Cytobiologica

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1999

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tom 37

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nr 2

3

Effect of goitrogenic alliloisothiocyanate on thyroid hormones and serum lipid profile in rats

100%

Kostogrys R. B., Pisulewski P. M., Pecio A.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 1

The objectives of the present study were to estimate the effects of increasing levels of the goitrogenic alliloisothiocyanate on body weight, thyroid hormones, morphology of thyroid follicles, and serum lipoproteins concentration. Twenty-four, growing male rats of Wistar strain were randomly assigned to four experimental groups (I – 0, II – 2, III – 4, and IV – 6 mg alliloisothiocyanate per kg body weight). For 20 days the rats were fed restricted amounts of AIN’93G diets and had free access to distilled water. Animal body weight was recorded weekly. No visible signs of alliloisothiocyanate toxicity were observed in this study. Feeding graded amounts of alliloisothiocyanate to rats resulted in insignificantly decreased serum concentrations of fT4 (by 30.5%). No significant effect of dietary alliloisothiocyanate on TSH concentrations was noted. In addition, alliloisothiocyanate altered thyroid follicle morphology as indicated by both hyperplasia and hypertrophy of the follicular epithelial cells. Goitrogenic alliloisothiocyanate was found to decrease serum total cholesterol and serum LDL-cholesterol (24% and 30%, respectively).

4

A study of the interaction of the C-reactive protein monomer with the U937 monocyte

84%

Zhao J., Shi X.-H.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 3

485-495

C-reactive protein (CRP) has two structurally distinct isoforms, the CRP pentamer and the CRP monomer. A role for the CRP monomer in atherosclerosis is emerging, but the underlying mechanisms are only beginning to be understood. Monocytes are an important contributor to atherosclerosis, and foam cell formation is the hallmark of atherogenesis. However, whether the CRP monomer can directly interact with the monocytes and modulate their responses remains unknown. Furthermore, although FcγRIII (CD16) has been identified as the receptor for the CRP monomer on neutrophils, its role in mediating the CRP monomer’s biological effects in other cell types has been questioned. In this study, we investigated the interaction of the CRP monomer with the monocytes using the U937 monocytic cell line. The CRP monomer specifically binds to U937 cells. This binding is unique in that it is independent of FcγRs and insensitive to protease digestion of the cell surface proteins. Further assays revealed that the CRP monomer directly incorporates into the plasma membrane. Interestingly, the presence of the CRP monomer efficiently retards oxidized low-density lipoprotein-induced foam cell formation of PMA-differentiated U937 macrophages and peripheral blood monocytic cell-derived macrophages. These findings provide additional evidence for the notion that the CRP monomer is an active CRP isoform that plays a role in atherogenesis via the direct modulation of the behavior of the monocytes.

5

Components of metabolic syndrome and their impact on fetal growth in women with gestational diabetes mellitus

84%

Zawiejska A., Wender-Ozegowska E., Brazert J., Sodowski K.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 4

5-18

6

Distribution of apolipoprotein Al-containing lipoprotein subclasses in plasma of normolipidemic subjects

84%

Atmeh R. F., Kasasbeh A. Z., Abu Odeh M. R.

Acta Biochimica Polonica

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2010

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tom 57

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nr 3

The distribution of apoA-I among apoA-I-containing lipoprotein (AI-Lp) subclasses in plasma was studied by immunoblotting utilizing agarose gel matrix incorporating anti-apoA-I as the transfer medium. Nine AI-Lp subclasses were detected in the plasma of normolipidemics, with relative molecular masses ranging from 70 000 to ≥ 354 000 and diameters from 7.12 to ≥ 11.6 nm. The mass distribution of AI-Lp subclasses was significantly different between males and females, and some subclasses increased gradually with age while others decreased. There was a significant strong positive correlation between subclass 1 (Mr 70 000-75 000) and subclass 3 (Mr 105 000-126 000) in all subjects and age groups. Analysis of similar AI-Lp or HDL subclasses reported in the literature showed variability in the sizes reported by various workers. This stresses the need for a unified classification of such subclasses, and this work contributes to this direction. The quantitative nature of the method used in this work compared with the semiquantitative approaches used earlier makes it a better method for the study of the quantitative changes of the subclasses in various physiological and pathological states. The method helps to generate ideas for in vitro and in vivo studies of apoA-I exchange among subclasses and in vivo kinetic studies. Conclusion. Plasma level of the AI-Lp subclasses varied quantitatively with age and gender, and strong correlations were detected between some subclasses. This work contributes to a better classification of AI-Lp subclasses according to their size. Comparison of the method used here with the methods reported in the literature revealed its advantages.

7

Mechanism of cell infection with hepatitis C virus [HCV] - a new paradigm in virus-cell interaction

84%

Budkowska A.

Polish Journal of Microbiology

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2009

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tom 58

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nr 2

93-98

Hepatitis C virus (HCV) is an enveloped, single-stranded RNA virus, belonging to the Flaviviridae family. HCV infection is a major cause of chronic hepatitis worldwide, leading to steatosis, liver cirrosis and hepatocellular carcinoma. Significant advances in understanding the mechanisms of HCV infection have been made since the development of a cell culture system reproducing the complete HCV cell cycle in vitro. HCV represents a new paradigm in interactions between the virus and its target cell, the human hepatocyte, due to the central role of lipoproteins in the HCV life cycle. Very low density lipoproteins are required for virus particle assembly and secretion. Upon the release, the infectious virus circulates in the blood as triglyceride-rich particles and infects cells using lipoprotein-receptor dependent mechanisms. HCV cell entry is a multi-step process: heparan sulphate and/or low-density lipoprotein receptor are cell surface factors mediating an initial virus attachment; subsequent virus interaction with tetraspanin CD81 and the human scavenger receptor SR-BI, the main HCV receptors, triggers virus movement to the tight junctions and its uptake via Claudin-1 and occludin. Another originality of HCV is that initiation of productive infection requires dynamic microtubules. Whereas other viruses use kinesin or dynein-dependent transport, HCV exploits mechanisms driven by microtubule polymerization to efficiently infect its target cell, in which virus nucleocapsid protein might play a particular role. An improved of understanding of the cellular-events involved in HCV cell entry and transport, leading to the initiation of productive HCV infection, may reveal novel targets for anti-viral interventions.

8

Mechanism of anchoring proteins on the cell envelope

84%

Pietraszek P. P., Walczak P. P.

Biotechnology and Food Science

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2011

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tom 75

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nr 1

9

Serum concentration of visfatin is decreased in patients with chronic heart failure

84%

Straburzynska-Migaj E., Pilaczynska-Szczesniak L., Nowak A., Straburzynska-Lupa A., Sliwicka E., Grajek S.

Acta Biochimica Polonica

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2012

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tom 59

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nr 3

Background. There is an increasing interest in the role of adipocytokines in cardiovascular pathophysiology. Aim. The aim of the study was to compare visfatin levels, a novel adipokine, in patients with heart failure (HF) due to the left ventricular systolic dysfunction with those in age- and body mass index (BMI) - matched healthy controls in relation to the parameters of glucose metabolism and high sensitivity C-reactive protein (hsCRP) levels. Material/Subjects and Methods. The study population consisted of 28 males with systolic HF referred for cardiopulmonary exercise testing, divided into two subgroups based on their NYHA class (HF patients NYHAI+II, n=17, and HF patients NYHAIII+IV, n=11), and 23 controls. The following indices were measured in a serum samples: visfatin, hsCRP, glucose and lipid metabolism parameters, and the insulin resistance index HOMAIR (homeostasis model assessment insulin resistance) was calculated. Results. Concentrations of visfatin and high-density lipoprotein cholesterol (HDL-cholesterol) in the HF subjects were significantly lower (p ≤ 0.01) than in controls. The Kruskal-Wallis test showed significant differences between three groups (controls and both subgroups of heart failure patients) in mean levels of visfatin, hsCRP, glucose, HOMAIR and HDL-cholesterol. Conclusion. Serum visfatin concentrations in patients with systolic HF, particularly with more advanced NYHA classes, are significantly lower in comparison to healthy controls and are independent of age or anthropometric and metabolic parameters.

10

Ionic channels formation in the course of endocytosis

84%

Lev A. A.

Biophysics of Membrane Transport. Supplement

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1994

|

tom 12

11

A note on the development of a rat model for cholesterol and lipoprotein metabolism - short report

84%

Pisulewski P. M., Kopec A., Cieslik E.

Polish Journal of Food and Nutrition Sciences

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2002

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tom 11/52

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nr 4

Twenty-four growing male rats of Wistar strain, weighing initially 120±3.5 g were randomly divided into four groups of six animals each and fed four different diets for 14 days: I - control diet (AIN-93G with soybean oil as a source of fat: 7 g/100 g), II - high-cholesterol diet (AIN-93G with soybean oil as a source of fat: 7 g/100 g + 1 g/100 g cholesterol), III - high-cholesterol diet (AIN-93G with lard as a source of fat: 7 g/100 g + + 1 g/100 g cholesterol), and IV - high-cholesterol diet (AIN-93G with lard as a source of fat: 7 g/100 g lard + 1 g/100 g cholesterol + 0.5 g/100 g cholic acid). On the day 14, the rats were anaesthetized and killed by withdrawing blood from the heart to obtain serum samples. The most striking increases (PcO.Ol) in the serum total and LDL+VLDL cholesterol were induced by the diet containing lard and supplemented with cholesterol + cholic acid (Group IV). Conversely, feeding the above diet to rats produced minimum (P<0.01) concentrations of the serum HDL cholesterol. It is concluded that the present animal model may be useful for evaluating the role of dietary factors in the development or regression of hypercholesterolemia.

12

Lipid metabolism parameters in patients with Alzheimer's disease and their first degree relatives

84%

Czyzewski K., Lalowski M. M., Pfeffer A., Barcikowska M.

Acta Neurobiologiae Experimentalis

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2001

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tom 61

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nr 1

13

Small high density lipoprotein subclasses: some of their physico-chemical properties and stability in solution

84%

Atmeh R. F., Issa A. E. O.

Acta Biochimica Polonica

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2005

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tom 52

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nr 2

Small high density lipoproteins (SHDL) contribute to the protection from atherosclerosis, but detailed information about their properties is not available yet. We isolated four of the smallest HDL subclasses that contain apoA-I alone, the small lipoprotein A-I (SLpAI), by their separation on gradient polyacrylamide gel followed by electroelution. Their physico-chemical properties were calculated from their displacement in non-denaturing gradient polyacrylamide gel under the effect of electrical potential. The properties are: Stokes’ radii 2.96–3.56 nm; molecular masses 42–70 kDa; net negative charge 7.2–13.5; surface charge densities 3139–4069 –esu ∙ cm–2; surface potentials 10.6–15.7 –mV; coefficients of friction 5.74–6.90 × 10–8 g ∙ s–1; and diffusion coefficients 5.76–6.94 × 10–7 cm2 ∙ s–1. We found that these particles were of low stability as they underwent molecular transformation into larger particles on storage. The estimated dimensions of these particles do not support ellipsoidal shape, therefore, the most probable shape is spherical; consequently, their hydrated characteristics were estimated. We conclude that these particles have high values of negative surface charge and diffusion coefficients, and are of low stability. Their small Stokes’ radii were similar to each other and they are spherical and highly hydrated.

14

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Beneficial effects of soybean protein and isoflavone extract supplementation on bone density and plasma lipids in females rats

84%

Ghanem K. Z.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 1

The main purpose of this study was to evaluate the effect of supplementation of a soybean protein concentrate (20%) on bone mass and plasma lipid profile of adult female rats as well as to test the effectiveness of a soybean isoflavonoid extract (0.5%) in preventing bone loss due to ovariectomy. Results showed that isoflavones separated from the soybean extract contain a higher amount of isoflavonoid glycosides (daidzin and genestin) than the aglycones (diadzein and genistein). Data obtained revealed that both plasma total cholesterol and high density lipoprotein cholesterol(HDL-C) of rats were not significantly changed due to the presence of the soybean protein concentrate (20%) in the diet. On the other hand, there was a significant decrease in plasma low density lipoprotein cholesterol (LDL-C) in soy protein group. Lipid peroxidation was significantly reduced due to feeding soybean protein concentrate (20%) of the diet (approximately 30% reduction). Rats fed the soybean protein concentrate (20%) diet had significantly higher mean bone densities (10% increase) of right tibia as compared with control (protein-based diet, 20%). The ovariectomized rats fed a diet containing the isoflavonoid extract had significantly higher mean bone densities (15% increase) and bone calcium of right tibia than ovariectomized rats fed the control diet. In conclusion, the present study demonstrated that soybean protein and isoflavones could have the potential to reduce the risks of postmenopausal osteoporosis and cardiovascular diseases in such women.

15

Accelerated oxidation of serum low density lipoprotein in gout and its improvement by allopurinol

84%

Tsutsumi Z., Yamamoto T., Takahashi S., Moriwaki Y., Yamakita J. I., Matsui K., Higashino K.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

16

Comparison of the localization and post-translational modification of Campylobacter coli CjaC and its homolog from Campylobacter jejuni, Cj0734c-HisJ

84%

Wyszynska A., Tomczyk K., Jagusztyn-Krynicka E. K.

Acta Biochimica Polonica

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2007

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tom 54

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nr 1

Campylobacte is an asaccharolytic microorganism which uses amino acids as a source of carbon and energy. CjaC/HisJ is a ligand-binding protein, a component of the ABC transport system. Campylobacter CjaC/HisJ is post-translationally modified by glycosylation. The number of glycosylation motifs present in the CjaC protein is species-specific. C. coli CjaC has two and C. jejuni one motif (E/DXNYS/T) which serves as a glycan acceptor. Although the two C. coli CjaC motifs have identical amino-acid sequences they are not glycosylated with the same efficiency. The efficacy of CjaC glycosylation in Escherichia coli containing the Campylobacter pgl locus is also rather low compared to that observed in the native host. The CjaC localization is host-dependent. Despite being a lipoprotein, CjaC is recovered in E. coli from the periplasmic space whereas in Campylobacter it is anchored to the inner membrane.

17

Free thyroid hormones and cholesterol in follicular fluid of bovine ovaries

84%

Blaszczyk B., Stankiewicz T., Udala J., Gaczarzewicz D., Lasota B., Blaszczyk P., Szymanska A., Szymanska-Pasternak J.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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nr 2

18

Ozone-induces changes in 1H NMR spectrum of lipoproteins of rabbits with atherosclerosis

84%

Kopieczna-Grzebieniak E., Drozdz M., Sroczynski J., Tarnawski R., Piskorska D., Grabowska-Bochenek R., Wesolowski W.

Current Topics in Biophysics

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1994

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tom 18

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nr 2

19

Lipids and lipoproteins in blood serum of calves receiving Yea-Sacc1026 dietary supplement

84%

Pysera B., Opalka A.

Journal of Animal and Feed Sciences. Supplement

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2001

|

tom 10

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nr 2

20

Decomposition of lipid hydroperoxides enhances the uptake of low density lipoprotein by macrophages

84%

Babiy A. V., Gebicki J. M.

Acta Biochimica Polonica

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1999

|

tom 46

|

nr 1

This study examined the roles of low-density lipoprotein (LDL) lipid oxidation and peroxide breakdown in its conversion to a form rapidly taken up by mouse peritoneal macrophages. Oxidation of the LDL without decomposition of the hydroperoxide groups was performed by exposure to gamma radiation in air-saturated solutions. Virtually complete decomposition of the hydroperoxides was achieved by treatment of the irradiated LDL with Cu2+ under strictly anaerobic conditions. No uncontrolled LDL uptake by macrophages occurred when the lipoprotein contained less than 150 hydroperoxide groups per particle. More extensively oxidized LDL was taken up and degraded by mouse macrophages significantly faster than the native lipoprotein. The uptake was greatly enhanced by treatment of the oxidized LDL with Cu2+. A significant proportion of the LDL containing intact or copper-decomposed LDL hydroperoxide groups accumulated within the macrophages without further degradation. Treatment of the radiation-oxidized LDL with Cu2+ was accompanied by aggregation of the particles. Competition studies showed that the oxidized LDL was taken up by macrophages via both the LDL and the scavenger receptors, whereas the copper-treated lipoprotein entered the cells only by the scavenger pathway. Phagocytosis also played an important role in the metabolism of all forms of the extensively modified LDL. Our results suggest that minimally-oxidized LDL is not recognized by the macrophage scavenger receptors unless the lipid hydroperoxide groups are decomposed to products able to derivatize the apo B protein.

Ograniczanie wyników

High density lipoprotein and low density lipoprotein-mediated changes in cultured human skin fibroblasts

Ultrastructural studies of the liver during experimental hypercholesterolaemia

Effect of goitrogenic alliloisothiocyanate on thyroid hormones and serum lipid profile in rats

A study of the interaction of the C-reactive protein monomer with the U937 monocyte

Components of metabolic syndrome and their impact on fetal growth in women with gestational diabetes mellitus

Distribution of apolipoprotein Al-containing lipoprotein subclasses in plasma of normolipidemic subjects

Mechanism of cell infection with hepatitis C virus [HCV] - a new paradigm in virus-cell interaction