Wyniki wyszukiwania - AGRO

1

Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv.viciae

100%

Izmailov S. F., Zhiznevskaya G. Y., Kosenko L. V., Troitskaya G. N., Kudryavtseva N. N., Borodenko L. I., Dubrovo P. N., Russa R., Pietras H., Lorkiewicz Z.

Acta Biochimica Polonica

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1999

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tom 46

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nr 4

Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.

2

Characterization of two lipopolysaccharide types isolated from Rhizobium galegae

100%

Rasanen L. A., Russa R., Urbaniak T., Choma A., Mayer H., Lindstrom K.

Acta Biochimica Polonica

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1997

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tom 44

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nr 4

Lipopolysaccharides (LPS) of Rhizobium galegae, a symbiotically nitrogen-fixing species of root-nodule bacteria, were isolated by the phenol-water method from strain HAMBI 1461, the LPS of which resembled enterobacterial smooth type LPS, and from strains HAMBI 1174 and HAMBI 1208, the LPSs of which resembled rough type LPS. The results of PAGE analysis of LPSs, Bio-Gel P2 gel filtration of polysaccharide fractions and the presence of deoxysugars and 4-O-methyl-deoxysugar both in the rough and smooth LPSs suggested that rough LPS contained a short O-antigenic polysaccharide for which we propose the name short O-chain LPS. Accordingly, the smooth LPS is called long O-chain LPS. Despite of the differences in the structure of LPS of R. galegae, all strains were equally effective in nodulating their hosts. The short O-chain LPS of R. galegae showed many features similar to those of phylogenetically related agrobacteria.

3

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Selective brain cooling in the ox during E.coli LPS induced fever

100%

Lachowski A., Chesy G.

Bulletin of the Veterinary Institute in Puławy

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1994

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tom 38

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nr 2

4

Level of interleukin-1beta and clinical signs after endotoxin injection in stallions

88%

Danek J.

Bulletin of the Veterinary Institute in Puławy

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2002

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tom 46

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nr 2

5

Central and peripheral neural aspects of gastroprotective and ulcer healing effects of lipopolysaccharides

88%

Konturek P. C., Brzozowski T., Meixner H., Ptak A., Hahn E. G., Konturek S. J.

Journal of Physiology and Pharmacology

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2001

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tom 52

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nr 4,1

Lipopolysaccharides (LPS) are major components of the outer membrane of Gram-- negative bacteria playing a central role as potent endotoxins in the pathogenesis of endotoxic shock. Although large amounts of endotoxin may produce hemorrhagic lesions in the stomach, the possible gastroprotective effect of central or peripheral LPS against the acute gastric lesions has not been extensively studied. The aim of the present study was to compare the effect of intracerebroventricular (i.c.v.) and parenteral (i.p.) injection of LPS against gastric lesions induced by 100% ethanol. Male Wistar rats were treated either with a) vehicle (control); b) E-coli-LPS in various concentrations (1—10 µg/kg i.c.v or 0.1—40 mg/kg i.p.) followed 30 min later by 100% ethanol. The effects of pretreatment with nonselective inhibitor of nitric oxide synthase (L-NAME, 20 mg/kg i.g.) or selective inhibitor of inducible nitric oxide synthase, L-NIL (30 mg/kg i.g) on the gastroprotection induced by LPS was investigated. One hour after ethanol application, the gastric blood flow (GBF) and the area of gastric lesions were determined. In addition, the mucosal expression of iNOS, cNOS and leptin was assessed using RT-PCR. LPS applied i.c.v. or i.p. dose dependently reduced gastric lesions induced by ethanol and this effect was similar to that observed after the administration of NO donor (SNAP). LPS-induced protection was significantly abolished by L-NAME and significantly attenuated by the selective inhibitor of iNOS (L-NIL). The expression of cNOS was detected in vehicle treated gastric mucosa and did not change after LPS administration. iNOS was not detectable in intact mucosa but its expression dose-dependently increased after the LPS administration. The i.c.v. administration of LPS did not upregulate further the iNOS expression, and dose-dependently inhibited the leptin mRNA expression in gastric mucosa. We conclude that LPS applied centrally or peripherally protects gastric mucosa against ethanol-induced damage through an increase in gastric microcirculation mediated by NO due to overexpression of iNOS. Transcriptional downregulation of leptin in gastric mucosa is probably due to the increased leptin release induced by the intracerebroventricular application of lipopolysaccharide.

6

Preparation of endotoxin-free bacteriophages

88%

Boratynski J., Syper D., Weber-Dabrowska B., Lusiak-Szelachowska M., Pozniak G., Gorski A.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 2

Bacteriophages (phages) are bacterial viruses that interact with bacterial walls and invade bacterial cells. Moreover, they disturb bacterial metabolism and lead to bacteria lysis. In the case of Gram-negative bacteria crude phage cultures, apart from the phages themselves, the bacterial debris, bacterial proteins and nucleic acids contain endotoxins. These endotoxins (lipopolysaccharides) posses a high degree of toxicity in vitro and in vivo, and their removal is essential for safety in antibacterial bacteriophage therapy. An effective, scaleable purification of bacteriophages from endotoxins was accomplished by sequential ultrafiltration through polysulfone membrane (30 nm) followed by chromatography on sepharose 4B and Matrex Cellulofine Sulfate. The phage fraction after gel filtration chromatography routinely contained endotoxins in the 150-2500 EU/mL range. The procedure yielded bacteriophages contaminated with as little as 0.4-7 EU/ml (Limulus assay). This value lies within the permitted level for intravenous applications (5 EU/kg/h by European Pharmacopoeia, 1997)

7

Role of prostaglandins and nitric oxide in the lipopolysaccharide-induced ACTH and corticosterone response

88%

Gadek-Michalska A., Bugajski J.

Journal of Physiology and Pharmacology

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2004

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tom 55

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nr 3

This study was designed to determine the role of endogenous prostaglandins (PG) and nitric oxide (NO) in the lipopolysaccharide (LPS)-induced ACTH and corticosterone secretion in conscious rats. LPS (0.5 and 1 mg/kg) given i.p. stimulated the hypothalamic-pituitary-adrenocortical (HPA) activity measured 2 h later. A non-selective cyclooxygenase inhibitor indomethacin (10 mg/kg i.p.), piroxicam (2 mg/kg i.p.), a more potent antagonist of constitutive cyclooxygenase (COX-1) and compound NS-398 (2 mg/kg i.p.), a selective inhibitor of inducible cyclooxygenase (COX-2) given 30 min before LPS (1 mg/kg i.p.) significantly diminished both the LPS-induced ACTH and corticosterone secretion. COX-2 blocker was the most potent inhibitor of ACTH secretion (72.3%). Nw-nitro-L-arginine methyl ester (L-NAME 2 and 10 mg/kg i.p.), a non-selective nitric oxide synthase (NOS) blocker given 15 min before LPS did not substantially alter plasma ACTH and corticosterone levels 2 h later. Aminoguanidine (AG 100 mg/kg i.p.), a selective inducible nitric oxide synthase (iNOS) inhibitor, considerably enhanced ACTH and corticosterone secretion induced by a lower dose (0.5 mg/kg) of LPS and did not significantly alter this secretion after a larger dose (1 mg/kg) of LPS. L-NAME did not markedly affect the indomethacin-induced inhibition of ACTH and corticosterone response. By contrast, aminoguanidine abolished the indomethacin-induced reduction of ACTH and corticosterone secretion after LPS. These results indicate an opposite action of PG generated by cyclooxygenase and NO synthesized by iNOS in the LPS-induced HPA-response.

8

Immunochemical studies on R mutants of Yersinia enterocolitica O:3

88%

Radziejewska-Lebrecht J., Skurnik M., Shashkov A. S., Brade L., Rozalski A., Bartodziejska B., Mayer H.

Acta Biochimica Polonica

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1998

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tom 45

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nr 4

Three mutants of Yersinia enterocolitica O:3, namely: YeO3-R1, YeO3-RfbR7 and YeO3-c-trs8-R were classified on the basis of sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS/PAGE) profile of isolated lipopolysaccharides (LPS) as belonging to the Ra- (the first) and the Rc-type (the other two mutants). Methylation analysis, in addition to 13C and 1H NMR studies of purified core oligosaccharides revealed structures similar to those established previously for the full core of Y. enterocolitica O:3 in the case of the Ra mutant, and identical to that reported for the Rc mutant Ye75R, in the case of the two other mutants. The O-specific sugar, 6d-l-altrose, which forms a homopolymeric O-chain, was present in small amounts in all three LPS preparations, as well as in the core oligosaccharide preparations along with the Ra and the Rc sugars, characteristic of the Y. enterocolitica O:3 core. This result is in line with genetic data, indicating that it is the inner core region which is the receptor for the O-specific chain in Y. enterocolitica O:3. This region seems likewise to be the anchoring region for the enterobacterial common antigen (ECA), as shown by SDS/PAGE/Western blot analysis with monoclonal antibodies against ECA. In addition, we also demonstrated that the Ye75R mutant Rc and its parental strain Ye75S, both were ECA-immunogenic strains. So far, ECA-immunogenic strains, i.e. those with LPS-linked ECA, were only identified in E. coli mutants of the R1, R4 and K-12 serotype.

9

Carvedilol and adrenergic agonists suppress the lipopolysaccharide-induced NO production in RAW 264.7 macrophages via the adrenergic receptors

75%

Pekarova M., Kralova J., Kubala L., Ciz M., Papezikova I., Macickova T., Pecivova J., Nosal R., Lojek A.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 1

143-150

The interaction of adrenergic agonists and/or antagonists with the adrenergic receptors expressed on immunologically active cells including macrophages plays an important role in regulation of inflammatory responses. Our study investigated the effects of carvedilol, a unique vasodilating b-adrenergic antagonist, and endogenous adrenergic agonists (adrenalin, noradrenalin, and dopamine) and/or antagonists (prazosin, atenolol) on lipopolysaccharide-stimulated nitric oxide (NO) production from murine macrophage cell line RAW 264.7. The production of NO was determined as the concentration of nitrites in cell supernatants (Griess reaction) and inducible nitric oxide synthase (iNOS) protein expression (Western blot analysis). Scavenging properties against NO were measured electrochemically. Carvedilol in a concentration range of 1, 5, 10 and 25 µM inhibited iNOS protein expression and decreased the nitrite concentration in cell supernatants. Adrenalin, noradrenalin or dopamine also inhibited the iNOS protein expression and the nitrite accumulation. Prazosine and atenolol prevented the effect of both carvedilol and adrenergic agonists on nitrite accumulation and iNOS expression in lipopolysaccharide-stimulated cells. These results, together with the absence of scavenging properties of carvedilol against NO, imply that both carvedilol and adrenergic agonists suppress the lipopolysaccharide-evoked NO production by macrophages through the activation and modulation of signaling pathways connected with adrenergic receptors.

10

Visfatin plays a significant role in alleviating lipopolysaccharide-induced apoptosis and autophagy through PI3K/AKT signaling pathway during acute lung injury in mice

75%

Wu X.-T., Ansari A. R., Pang X.-X., Li H.-Z., Zhang Z.-W., Luo Y., Arshad M., Song H.

Archivum Immunologiae et Therapiae Experimentalis

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2019

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tom 67

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nr 4

11

The effect of omega-3 fatty acids on expression of connexin-40 in Wistar rat aorta after lipopolysaccharide administration

75%

Frimmel K., Vlkovicova J., Sotnikova R., Navarova J., Bernatova I., Okruhlicova L.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 1

12

Human complement activation by smooth and rough Proteus mirabilis lipopolysaccharides

75%

Kaca W., Arabski M., Fudala R., Holmstrom E., Sjoholm A., Weintraub A., Futoma-Koloch B., Bugla-Ploskonska G., Doroszkiewicz W.

Archivum Immunologiae et Therapiae Experimentalis

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2009

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tom 57

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nr 5

383-391

13

Lack of TCRalpha beta plus CD8plus and TCRgamma delta plus lymphocytes ameliorates LPS induced orchitis in mice - preliminary histological observations

75%

Sliwa L., Macura B., Majewska-Szczepanik M., Szczepanik M.

Folia Biologica

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2014

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tom 62

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nr 1

14

Endotoxin elimination in patients with septic shock: an observation study

75%

Adamik B., Zielinski S., Smiechowicz J., Kubler A.

Archivum Immunologiae et Therapiae Experimentalis

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2015

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tom 63

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nr 6

15

Crystals of recombinant human tumor necrosis factor

75%

Joachimiak A., Giel M., Klysik J. E., Stec W. J., Barciszewski J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1991

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tom 39

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nr 1

16

Seasonal modulation of interferon response in spotted sousliks [Spermophilus suslicus]

75%

Kandefer-Szerszen M., Meczynski S., Borowska L., Szuster-Ciesielska A., Rzeski W.

Archivum Immunologiae et Therapiae Experimentalis

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1994

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tom 42

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nr 5-6

17

Rat epididymal epithelial cells produce nitric oxide upon concomitant lipopolysaccharide and interferon-gamma stimulation

75%

Kurzawa R., Barcew-Wiszniewska B., Skowron J.

Folia Histochemica et Cytobiologica. Supplement

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1996

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tom 34

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nr 1

18

Effect of unilateral, intraovarian infusions of Escherichia coli endotoxin on ovarian morphology and blood hormonal profiles in gilts

75%

Jana B., Kucharski J., Kotwica J.

Polish Journal of Veterinary Sciences

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2000

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tom 03

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nr 2

The aim of this study was to determine the influence of unilateral, intraovarian infusions of Escherichia coli endotoxin (LPS, lipopolysaccharide, serotyp 055:B5) on the concentration of progesterone (P4), testosterone (T), estradiol-17ß (E2) and luteinizing hormone (LH) in peripheral and ovarian blood plasma of gilts. The morphology of ovaries was also estimated. Fourteen sexually matured gilts with controlled estrous cycle were used. The animals were randomly divided into two groups: I (treated; n=7), and II (control; n=7). In the group I, 2 mg of LPS in 1 ml of saline was infused into the hilus of one ovary from the 15th to the 19lh day of the estrous cycle, twice a day (at 06:00 and 18:00). At the same time, 1 ml of saline was infused into the hilus of the contralateral ovary and into both ovaries of the control gilts (Gr. II). From the 15lh to the 7th day of the next estrous cycle blood samples from the jugular vein were collected with various frequency. Additionally, on the 7th day, during the laparotomy, blood samples from the utero-ovarian vein of each ovary were collected and then both ovaries were dissected out to estimate their morphology. Plasma hormone concentrations were determined by radioimmunoassay (RIA). Macroscopic investigation revealed the presence of cysts in both ovaries after unilateral, intraovarian LPS infusions but the number of cysts in the LPS-treated ovaries was higher (P < 0.05) than that in the contralateral ones. In LPS-infused ovaries, numbers of follicles and corpora lutea were lower (P < 0.05) than those in the contralateral and control ovaries. In LPS-treated gilts, P4 concentrations in peripheral blood was increased (P < 0.05-0.001) as compared to that found in the control group in the period from the 21lh to the 7lh day of the next estrous cycle. After LPS infusions, the level of P4 in blood plasma from the utero-ovarian vein of both ovaries was higher (P < 0.05) than that observed in the control group. Moreover, the concentration of P4 was higher (P < 0.05) in LPS-infused ovary than that found in the contralateral one. During the consecutive days of the study, the T level in peripheral and ovarian blood remained unchanged in both the control and LPS-treated groups. In the gilts receiving LPS, the peripheral level of E2 decreased (P < 0.01-0.001) as compared to that found in the control animals from the 20th to the 7lh day of the next estrous cycle. On the 7lh day, the plasma concentration of E2 in the utero-ovarian vein was lower in LPS-treated gilts (P < 0.05) than that found in the control group. This study revealed that infusions of LPS into the hilus of one ovary in gilts caused morphological changes in both ovaries and deviations from normal plasma hormonal profiles. These disturbances partly depended on the location of LPS infusions. The results obtained suggest that the pathologically changed ovary can affect the pituitary function and steroidogenesis of the contralateral ovary what leads to disturbances in the physiologic rythm of the estrous cycle in females.

19

Effects of administration of Escherichia coli lipopolysaccharides and flunixin meglumine on semen quality in the stallion

75%

Danek J.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 4

20

Psychosocial stress affects the involvement of prostaglandins and nitric oxide in the lipopolysaccharide-induced hypothalamic-pituitary-adrenal response

75%

Gadek-Michalska A., Spyrka J., Bugajski J.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 2

The role of prostaglandins and nitric oxide (NO), generated after peripheral lipopolysaccharide (LPS) administration, in the adaptation of hypothalamic-pituitary-adrenal (HPA) axis under stressful circumstances remains to be elucidated. The aim of the present study was to assess the effect of chronic repetitive restraint or social crowding stress on the involvememt of nitric oxide and prostaglandins in the LPS-induced pituitary-adrenocortical response. Male Wistar rats were restrained in metal tubes 2x10 min/day or crowded in cages for 7 days prior to treatment. All compounds were injected i.p., cyclooxygenase (COX) and nitric oxide synthase (NOS) inhibitors 15 min before LPS. Two hrs after injection LPS induced a significant increase in ACTH and corticosterone secretion. Repeated restraint impaired more potently than crowding stress the LPS-induced HPA-response. Indomethacin, a non-selective COX inhibitor, considerably reduced the LPS-induced HPA response in non-stressed rats and to a lesser extent diminished this response in repeatedly restrained or crowded rats. Neuronal NOS inhibitor, Nw-nitro-L-arginine decreased the LPS-induced HPA response, more potently in control than crowded rats. Aminoguanidine, an iNOS inhibitor, diminished the LPS-elicited ACTH response in crowded rats. These results indicate that prostaglandins and NO generated by neuronal and inducible NOS are involved in the LPS-induced HPA axis response under basal conditions and during its adaptation to chronic social stress circumstances.

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