An investigation of plants reach in lipophylic compounds and searching of sufficient raw material are being conducted due to rising scientists’ interest. Among them, we can count the plants of Fabaceae and Poaceae families. With the use of a gas-liquid chromatography method (GLC), the qualitative composition and the quantitative content of fatty acids and tocopherols in the lipophylic fraction of Glycine hispida, as well as sterols and fatty acids in the lipophylic fractions of fruits and grass of Setaria italica have been determined. In addition steroid and fatty-acid compositions of fruits of Setaria italica have been studied using the methods of gas chromatography/mass-spectrometry (GC/MS).