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Mechanical damage is one of the causes of great loss in the quality of soybean seeds during harvest and processing. Considerable interest exists in the lignin since its deposition in the seed coat tissue provides mechanical resistance and protects the cell against microorganisms. In addition, peroxidases might be involved in the oxidation of cinnamyl alcohols prior to their polymerization during lignin formation. Thus, the aim of the present work was to analyze the lignin contents and peroxidases activities of six Brazilian soybean cultivars (Savana, Paranagoiana, FT-10, Santa Rosa, Doko and Parana) and their relationships with the mechanical damage. Results showed that the lignin content and peroxidase activity in the seed coat significantly differed among the soybean cultivars. Cultivars Doko and Parana had the highest contents of lignin and peroxidases activities while the other cultivars had lowest lignin contents and enzyme activtties. Lignin content and peroxidase activity may be reasonable indicators of resistance to mechanical damage in soybean seeds.
Impact of environmental pollution caused by sulphur mining on the chemical composition of wood and bark of birch (Betula pendula Roth.) Content of extractives, cellulose, !% NaOH soluble substances and lignin were examined. Samples were gained from cca. 70-year old birch (Betula pendula Roth.) grown in the environment polluted by sulfur mining industry. 200mm height disks were cut in the butt-end, middle and top section of the trunk. Disk from the main branch was also collected. Bark was collected from butt-end section. On the cross-section from butt-end and middle part of the trunk following zones were marked: outer, center and pith adjacent wood. On the cross-section from the top part wood was divided into outer and pith adjacent. Only one zone was marked in branch. Results show that environmental pollution caused by sulfur mining industry influences the content and distribution of extractives on the cross- and longitudinal sections. It also causes the increase of extractives content in bark. Content and distribution of 1% NaOH soluble substances are also changed on both cross-sections. Environmental pollution decreased lignin content in wood and increased this content in bark from butt-end section.
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