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1

Wplyw stresu transportowego na stopien wrazliwosci leukocytow na dzialanie leukotoksyny Mannheimia haemolytica

100%
Urban-Chmiel R., Puchalski A., Wernicki A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 09
1045-1047
The purpose of the study was estimating the viability and susceptibility effect of leukocytes isolated from cattle before and after the transportation in vitro on M. haemolytica leukotoxin (Lkt) cytotoxicity. 40 Simentaler heifers that were transported by truck a distance of 1700 km for 72 hours were used in the experiment. The material for the study was the blood (40 samples) collected on heparin directly before and after transportation. In relation to leukocytes the examination of susceptibility on cytotoxic effect of Lkt has been carried out with the use of MTT (microtitration assay) and the viability of leukocytes after 1, 2, 3 and 6 hour of incubation. The results obtained in the cell viability test did not show statistically significant differences (P≥0.05) in 1st and 2nd hour of incubation in leukocytes obtained from heifers before and after transportation. After the 1st hour of incubation the percentage of leukocyte viability was on a very high level and showed 87% in both groups of animals. The significantly lower cell viability values in comparison to leukocytes isolated from animals before the transportation was observed in the transported heifers from 3rd to 6th hour of incubation. The analysis of the results obtained by MTT test indicated statistically significant differences in the susceptibility of leukocytes for cytotoxic activity of Lkt. The average values of toxic activity of Lkt in relation to leukocytes isolated before and after transportation was 79% and 92% respectively. The lytic activity of Lkt for 50% of the cell population referred as 1 unit (1U) was observed in Lkt concentration 15 mg/ml (leukocytes before transportation) and 7.5 mg/ml (after the transportation). The increase of susceptibility of leukocytes isolated after transportation on the cytotoxic effect of leukotoxin suggest the significant influence of transporting stress on the increase of respiratory diseases caused by M. haemolytica strains.
2

Wplyw leukotoksyny Mannheimia haemolytica A1 na odpowiedz zapalna u cielat

100%
Bednarek D., Szymanska-Czerwinska M., Dudek K.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 06
400-404
The aim of this study was to estimate the effect of leukotoxin (Lkt) Mannheimia haemolytica A1 on the inflammatory response in calves. The study was performed on 12 clinically healthy, Black and White Lowland breed calves, aged 8 weeks. Each of the experimental animals received a single dose of 25µg of Mannheimnia haemolytica A1 Lkt intravenously. The Lkt treated calves were compared with non-treated controls before (0) and 1, 2, 3, 4, 5, 6, 12, 24, 48 and 72 h after the treatment. The following parameters were assayed: concentrations of eicosanoids such as: prostaglandins E₂ and F₂α (PGE₂, PGF₂α), thromboxaneB₂(TXB₂) and leukotriene B4 (LTB₄), as well as selected acute phase proteins (CRP, Cp, Tf, Hp, SAA). The obtained results showed that the concentrations of some acute phase proteins, such as Hp and SA,A and also eicosanoids (PGE₂, PGF2α, TXB₂, LTB₄), were significantly higher in comparison to the controls especially during the first 3-6 h after the Lkt administration.
3

Skutecznosc natywnej i inaktywowanej leukotoksyny Mannheimia haemolytica w immunoprofilaktyce swoistej syndromu oddechowego owiec

84%
Mikucki P., Wernicki A., Puchalski A., Urban-Chmiel R.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 11
1302-1305
The purpose of this study was to compare the immunostimulating properties and prophylactic effects of native M. haemolytica leukotoxin (Lkt) which was inactivated by formaldehyde and glutaraldehyde. The study also estimated the neutralizing properties of antibodies induced by different forms of Lkt and their protective effects on experimental challenge in sheep. The results of the study suggest that the inactivation procedure has an important effect on immunogenic obtained toxins. Vaccines used in the immunized animals demonstrated an immunogenic presence which was observed in an increase of the specific antibody titers in sera following immunization. An increase in the neutralizing properties of antibodies contained in the sera of animals immunized by Lkt was also observed. The obtained results indicate the possibility of a more effective use of M. haemolytica Lkt isolated from bovine respiratory syndrome as a subunit of the vaccines already used in immunoprophylaxis of this disease in sheep.
4

Odpowiedź immunologiczna cieląt immunizowanych szczepionką na wpływ leukotoksyny (LKT) Mannheimia haemolytica A1

67%
Bednarek D., Dudek K., Urban-Chmiel R.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2008
|
tom 03
|
nr 2
5

Wplyw stresu transportowego na stopien podatnosci bydla i owiec na oddzialywanie leukotoksyny Mannheimia haemolytica

67%
Urban-Chmiel R.
Rozprawy Naukowe. Akademia Rolnicza w Lublinie
|
2007
|
nr 323
1-88
6

Paleczki z rodziny Pasteurellaceae. Rola w etiopatogenezie syndromu oddechowego bydla

67%
Bednarek D.
Weterynaria w Terenie
|
2008
|
tom 02
|
nr 3
6-8
7

Molekularna charakterystyka czynnikow wirulencji Actinobacillus actinomycetemcomitans

67%
Affek K., Jagusztyn-Krynicka E. K.
Postępy Mikrobiologii
|
2007
|
tom 46
|
nr 2
113-123
8

Znaczenie Pasteurella sp. w etiopatogenezie syndromu oddechowego bydła

67%
Bednarek D.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2008
|
tom 03
|
nr 2
9

Zakażenia Mannheimia haemolytica - ważny problem w odchowie bydła

67%
Dudek K., Bednarek D.
Weterynaria w Terenie
|
2013
|
tom 07
|
nr 3
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