Wyniki wyszukiwania - AGRO

1

Incorporation of stilbazolium merocyanines into resting and stimulated mononuclear leukocytes

100%

Frackowiak D., Wiktorowicz K., Cofta J., Niedbalska M., Latosinska M.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

Stimulated and resting mononuclear leukocytes were incubated with a stilbazolium merocyanine dye l-(6'-hydroxyhexyl)-4-[(4-oxocyclohexa-2,5-dienylidene)ethylide- ne]-l,4-dihydropyridine and immobilized in isotropic and stretched polyvinyl alcohol film. Polarized absorption, fluorescence and fluorescence excitation spectra were collected and the anisotropy of absorption and emission were calculated. Analysis of the spectra pointed to: i. the occurrence of perturbation of the membrane structure by incubation with the dye, and ii. influence of the blood serum addition, during the process of incubation with.the dye, on the efficiency of incorporation of merocyanine into the cells and the degree of the dye orientation in the membrane. A small fraction of the dye molecules introduced into resting cells was found oriented to a higher degree than a large fraction incorporated into stimulated cells. The incubation time longer than 15 min caused strong changes in the membrane structure both of the resting and stimulated cells.

2

Influence of various forms of dialyzable leukocyte extracts on rat adjuvant arthritis

88%

Stancikova M., Rovensky J., Pekarek J., Orvisky E., Blazickova S., Cech K.

Archivum Immunologiae et Therapiae Experimentalis

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1994

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tom 42

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nr 4

3

The spectral properties of stilbazolium merocyanine coplexes with Fe [II] and Fe [III] ions

88%

Cegielski R., Niedbalska M., Pajchrowski G., Manikowski H.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

In vivo imaging using liposomal phage display peptide derivates targeting integrins in activated leucocytes

88%

Ranta T. M., Valtanen H., Kaukinen S., Boerman O. C., Simpura I., Zhu Y., Medina O. P., Oyen W. J. G., Kairemo K.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.

5

Role of the p38 MAPK pathway in induction of iNOS expression in human leukocytes

75%

Jablonska E., Ratajczak W., Jablonski J.

Folia Biologica

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2008

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tom 56

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nr 1-2

83-89

6

Are changes in HSPA1A, HSPB1 and LDHb genes expression during physical performance ”till exhaustion” independent of their exercise possibility?

75%

Jastrzebski Z., Zychowska M.

Baltic Journal of Health and Physical Activity. The Journal of Gdansk University of Physical Education and Sport

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2014

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tom 06

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nr 4

Background: The aim of the study was to designate changes in the expression of HSPA1A, HSPB1 and LDHb in elite rowers after completing a test “till exhaustion” on a rowing ergometer. Finally, we searched for the answer whether there are significant correlations between the expression of the genes and anaerobic threshold (AnT) or the maximal oxygen uptake (VO2max). Material/Methods: The research was conducted on the sample of 9 Polish lightweight male rowers (23.7 ±3.77 yrs, 72.7 ±1.76 kg, 183.6 ±4.58 cm). To determine AnT and VO2max, the subjects performed the test “till exhaustion” with an increasing load on a rowing ergometer. Directly before and after the test, blood samples were collected from the ulnar vein in order to isolate genetic material. RNA was extracted from white cells of venous blood by the chemical method. 2 µg RNA for the reverse transcription was used and the expression of HSPA1A, HSPB1 and LDHb was determined by Real time PCR reaction. To assess the intensity of expression, the ∆∆Ct method was used. Results: The study showed an increased expression of HSPA1A and HSPB1 and a decreased one of LDHb. Moreover, post-training changes of the genes activity in white blood cells occurred immediately and could be determined directly after the termination of exertion. Conclusions: No significant correlations between the expression of the genes and anaerobic threshold (AnT), maximal oxygen uptake (VO2max) were stated.

7

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Effect of conglutinin on phagocytic activity of bovine granulocytes

75%

Dec M., Wernicki A., Puchalski A., Urban-Chmiel R., Radej S.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

In the present study we investigated the effect of bovine conglutinin on the phagocytic activity of leukocytes. We measured both the chemotactic activity of conglutinin and its effect on the internalization of zymosan particles and E. coli by granulocytes. We also assessed the binding of conglutinin to various microorganisms isolated from clinical cases in cattle. We showed that conglutinin binds strongly to the surface of yeast cells and to mannan-rich zymosan particles, while weak binding was observed in the case of the bacterial strains tested, including those whose O antigen is composed of mannan. Conglutinin (1-10 μg/ml) neither acts as a chemotactic factor for peripheral blood leukocytes nor affects ingestion of E. coli by granulocytes. However, as flow cytometry based assay showed, conglutinin (0.1-1 μg/ml) increased ingestion of zymosan expressed as mean fluorescence intensity (MFI) of positive cells.

8

Elastinolytic activity of horse leukocyte proteinases. Comparison with elastases from human leukocytes and porcine pancreas

75%

Potempa J., Korzus E., Dubin A., Silberring J.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

9

Nuclear import of arachidonate 5-lipoxygenase

75%

Brock T. G., Healy A. M.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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tom 48

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nr 6 Spec.Iss.

481-486

10

Flow cytometric assay in peripheral blood of dogs - reference values for leukocytes from Brazilian Beagles

75%

Fujiwara R. T., Bueno L. L., Vale A. M., Franca-Silva J. C., da Costa R. T., da Silva Quetz J., Machado-Coelho G. L., Reis A. B., Martins Filho O. A., Genaro O., Nascimento E., Mayrink W.

Polish Journal of Veterinary Sciences

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2005

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tom 08

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nr 1

11

Evaluation of peripheral blood leukocyte subpopulations by flow cytometry in calves treated with Mannheimia haemolytica leukotoxin

75%

Bednarek D., Urban-Chmiel R., Dudek K., Szymanska-Czerwinska M.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

199-203

The aim of the study was to evaluate the effect of Mannheimia haemolytica leukotoxin (Lkt) on cellular immune response in clinically-healthy calves given intravenously 25 µg per animal of M. haemolytica Al Lkt. The alternations of peripheral blood leukocytes were examined with a flow cytometry. The Lkt treated calves were compared with the non-treated controls before (0) and 1, 2, 3, 4, 5, 6, and 24 h after the treatment. The following parameters were assayed: white blood cell (WBC) count, percentage of polymorphonuclear leukocytes (PMNL), mid-size leukocytes, total percentage of lymphocytes and their subsets: CD2⁺ (T lymphocytes), CD4⁺ (T helper lymphocytes), and CD8⁺ (T suppressor/cytotoxic lymphocytes) with CD4⁺: CD8⁺ratio, and also WC4⁺ cells (B lymphocytes). The obtained results showed that in the treated calves, the WBC count, the percentages of PMNL, mid-size leukocytes, and some subpopulations of peripheral lymphocytes (CD2⁺, CD4⁺, CD8⁺) were significantly lower compared with the controls during the first of three hours of the experiment, and then the parameters increased and returned to the physiological level.

12

Humic-fatty acid preparation in growing rabbits nutrition

75%

Rzasa A., Mista D., Wincewicz E., Zawadzki W., Dobrzanski Z., Gelles A.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2014

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tom 17

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nr 3

13

Comparison of specificity of human and horse leucocyte proteinases with synthetic peptide substrates

75%

Dubin A., Potempa J., Schnebli H. P., Koj A.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

14

Abnormal eicosanoid-pattern of peripheral white-blood-cells in gastro-intestinal cancer

75%

Baenkler H. W., Schafer D.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 5

119-128

COX-inhibitors promote nasal polyps or bronchial asthma in individuals susceptible to an alteration of the pattern of the eicosanoids, especially leukotrienes and prostaglandins. This is associated with an abnormal release of eicosanoids from white blood cells. Since COX-inhibitors also protect from colorectal cancer an analogous association may be suggested. The study was performed to detect abnormal patterns of eicosanoids in white blood cells of patients with intestinal cancer compared to healthy controls. Seventy patients with intestinal cancer (stomach = 5; colon = 25; sigma = 18; rectum = 22) were compared to 62 healthy controls. Blood leukocytes from patients in complete long-lasting remission were incubated with diluent, arachidonic acid or acetylsalicylic acid. The synthesis of prostaglandin E2 and peptido-leukotrienes was quantified using competitive enzyme-immuno-assays and calculated for individual eicosanoid patterns. The mean basal and arachidonic- or acetylsalicylic acid-modulated PGE2 synthesis in patients was significantly higher than in controls (4.8-fold, 9.4-fold, 3.7-fold, respectively) whereas pLT was generally less elevated. We conclude that the eicosanoid-pattern of white-blood-cells from patients with intestinal cancer differs significantly from that in healthy individuals. This abnormal cellular metabolism may contribute to the manifestation of cancer and help to detect individuals at risk.

15

Morphology and phagocytic activity of heterophilic granulocytes in the rat and quail

75%

Kaczanowska-Taraszkiewicz E., Gromysz-Kalkowska K.

Zoologica Poloniae

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1997

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tom 42

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nr 1-4

16

Seasonal changes in the white blood cell count and blood cell sedimentation rate in the European bison Bison bonasus

75%

Gill J.

Acta Theriologica

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1992

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tom 37

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nr 3

17

Influence of the perinatal period on bovine interferon production

75%

Kandefer-Szerszen M., Kondracki M., Szuster-Ciesielska A., Rzeski W., Zdzisinska B., Cakala S.

Archivum Immunologiae et Therapiae Experimentalis

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1994

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tom 42

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nr 4

18

The effect of danazol on the activity of peritoneal macrophages

75%

Kurzawa R., Barcew-Wiszniewska B., Skowron J.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 2

19

Endotoxaemia in rats: role of leucocyte sequestration in rapid pulmonary nitric oxide synthase-2 expression

75%

Gebska A., Olszanecki R., Korbut R.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 2

Nitric oxide (NO), depending on the amount, time and source of generation may exert both, protective and deleterious actions during endotoxic acute lung injury (ALI). Evaluation of the expression and localization of NOS isoforms in the lung of lipopolysaccharide (LPS) - treated rats may contribute to understanding the role of NO in pathogenesis of ALI. Tissue samples (lung, heart, liver, kidney and spleen) as well as peripheral blood polymorphonuclear cells (PMNs) were collected from control male Wistar rats and LPS - treated animals, 15, 30, 60, 120 and 180 min after LPS injection (2 mg kg-1 min-1 for 10 minutes, i.v.). Levels of NOS-2 and NOS-3 mRNA and protein in tissues and PMNs were estimated by RT-PCR, Northern blotting and Western blotting. Additionally, myeloperoxidase (MPO) activity in tissue samples was assayed. NOS-3 mRNA as well as protein were detected in lungs of control animals; pulmonary NOS-3 expression was not influenced by LPS. The induction of NOS-2 mRNA in rat lungs and in PMNs isolated from peripheral blood was observed 15 minutes after LPS challenge. In contrast, increase of NOS-2 mRNA in the heart, kidneys, liver and spleen was observed 2-3 hours after LPS injection. In all tissues rise in NOS-2 mRNA was followed after 1-2 hours by increase of NOS-2 protein. Importantly, progressive leukocyte sequestration in the lung parenchyma that started as early as 15 min after LPS injection was revealed only in the lungs; in other organs no significant changes in MPO activity were detected up to 180 min after LPS injection. In conclusion, infusion of LPS caused much more rapid expression of NOS-2 in lungs as compared to the heart, kidneys, liver and spleen. Early induction of NOS-2 may depend on the LPS-stimulated rapid neutrophil sequestration within lung vasculature and fast induction of NOS-2 in sequestrated neutrophils.

20

Peripheral blood leukocytes and lymphocyte subpopulations as determined by flow cytometric measurements in healthy children

75%

Kawiak J., Rokicka-Milewska R., Zeman K., Hoser G., Derulska G., Fornalczyk-Wachowska E., Gosk B., Kantorski J., Pacholska J., Tchorzewski H., Pawelec K.

Folia Histochemica et Cytobiologica

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1995

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tom 33

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nr 1

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