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To determine whether behavioral sensitization produced by prolonged D-amphetamine administration affects susceptibility of nigrostriatal dopaminergic neurons to the neurotoxic actions of 6-hydroxydopamine (6-OHDA), rats were treated daily from the 23 rd day after birth for 11 consecutive days with D-amphetamine (1.0 mg/kg s.c.) or saline. On the last day of treatment, one group primed with D-amphetamine and one control group of rats were tested to confirm behavioral sensitization development. The remaining animals were additionally treated on the 34 th day (one day after the last D-amphetamine injection) with 6-OHDA HBr (300 µg in 10 µl i.c.v., salt form, half in each lateral ventricle) or its vehicle. Four weeks later the levels of dopamine (DA) and its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-metoxytyramine (3-MT), as well as 5-hydroxytrypatmine (5-HT) and its metabolite 5-hydroxyindoleacteic acid (5-HIAA) were assayed in the striatum, by HPLC/ED. In rats with behavioral sensitization, 6-OHDA reduced endogenous dopamine and its metabolites content to a comparable degree in comparison to controls. This finding indicates that presumed up-regulation of the dopamine transporter in the behaviorially sensitized rats did not increase the neurotoxicity of a high dose of 6-OHDA.
Jurkowlaniec E., Trojniar W. and Tokarski J.: The EEG activity after lesions of the diencephalic part of the zona incerta in rats, Acta Physiol. Pol. Neocortical and hippocampal EEG activity was recorded in 23 rats subjected to the bilateral electrolytic lesions of the diencephalic zona incerta (ZI). The aim was to find whether damage to ZI can replicate insomnia and disturbances in cortical EEG desynchronization and hippocampal theta rhythm found after lesions of the lateral hypothalamic (LH) area. No effect of the ZI lesions on waking-sleep cycle was found. The amplitude and frequency of cortical waves and hippocampal theta rhythm during waking were changed only in some rats. These changes were small, short-lasting and bidirectional (toward and increase or decrease in different subjects). Both the amplitude and frequency of paradoxical sleep theta were depressed in part of animals. Thus the marked EEG changes after LH lesions can not be attributed to simultaneous damage of the adjacent subthalamic region. However, the ZI seems to constitute a part of a larger system regulating cortical arousal and hippocampal theta rhythm.
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