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Unique analytical capabilities of laser scanning cytometry [LSC] that complement flow cytometry

100%

Deptala A., Bedner E., Darzynkiewicz Z.

Folia Histochemica et Cytobiologica

2001

tom 39

nr 2

Dissimilar effects of LY 294002 and PD 098059 in IGF-I- mediated inhibition of TGF-beta1 expression and apoptosis in bovine mammary epithelial cells

75%

Zarzynska J., Motyl T.

Journal of Physiology and Pharmacology. Supplement

2005

tom 56

nr 3

181-193

In mammary epithelial cells (MEC) TGF-ß1 is the auto-/paracrine growth inhibitor and inducer of apoptosis and therefore is considered as an important local regulator of mammary tissue involution. However, the mechanisms of controlled TGF-ß1 expression in the course of bovine mammary gland remodelling are still unclear. Recent study performed in this laboratory support the evidence that TGF-ß1 expression in bovine MEC is regulated by hormones of somatotropic axis (GH, IGF-I and somatostatin). Present study was focused on the contribution of IGF-I-induced signaling pathways in anti-TGF-ß1 and anti-apoptotic effects of IGF-I. Laser scanning cytometry was applied for the measurement of TGF-ß1 content and apoptotic cell number in bovine BME-UV1 MEC. Involution of the bovine mammary gland in vitro was modeled by decreasing the availability of FBS for bovine MEC. Reducing FBS content in the medium from 10% to 0.5% evoked highly significant increase of TGF-ß1 expression and increase of apoptotic cell number. IGF-I (50 ng/ml) completely abrogated FBS deficiency-induced TGF-ß1 expression and apoptosis in bovine MEC. In order to establish which of the IGF-I signaling pathways contributed to anti-TGF-ß1 and anti-apoptotic effects, the inhibitors of PI3-kinase - (LY 294002) and MEK- (MAPKK for ERK) (PD 098059) mediated signaling pathways were applied to our model. The results clearly showed that inhibition of PI3-K reverses the ability of IGF-I to suppress TGF-ß1 expression and apoptosis. An inhibition of ERK1/2 pathway even potentiated inhibitory effect of IGF-I on TGF-ß1 expression, but partially abrogated anti-apoptotic effect of IGF-I. In conclusion, the results of the study indicate that PI3-K/Akt pathway contributed significantly to the inhibition of TGF-ß1 expression by IGF-I, whereas both PI3-K/Akt and ERK1/2 pathways are involved in the anti-apoptotic effect of IGF-I in bovine MEC.

Application of primary cell cultures of laryngeal carcinoma and laser scanning cytometry in the evaluation of tumor reactivity to cisplatinum

63%

Klatka J., Paduch R., Pozarowski P., Pietruszewska W., Kupisz K., Trojanowski P., Rolinski J.

Folia Histochemica et Cytobiologica

2008

tom 46

nr 2

159-164

Ograniczanie wyników

2 Folia Histochemica et Cytobiologica

1 Journal of Physiology and Pharmacology. Supplement

1 Bedner E.

1 Darzynkiewicz Z.

1 Deptala A.

1 Klatka J.

1 Kupisz K.

1 Motyl T.

1 Paduch R.

1 Pietruszewska W.

1 Pozarowski P.

1 Rolinski J.

1 Trojanowski P.

1 Zarzynska J.

1 2008

1 2005

1 2001

Wyniki wyszukiwania

Unique analytical capabilities of laser scanning cytometry [LSC] that complement flow cytometry

Dissimilar effects of LY 294002 and PD 098059 in IGF-I- mediated inhibition of TGF-beta1 expression and apoptosis in bovine mammary epithelial cells

Application of primary cell cultures of laryngeal carcinoma and laser scanning cytometry in the evaluation of tumor reactivity to cisplatinum