Wyniki wyszukiwania

1

Alterations in the lactate dehydrogenase isoenzyme pattern after maximal running exercise

100%

Szade B., Poprzecki S., Klapcinska B., Sadowska-Krepa E., Grzesiok K., Iskra J., Sobiech K. A.

Polish Journal of Environmental Studies

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1998

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tom 07

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nr 6

2

Simplified solution of lactate dehydrogenase catalysed reaction

100%

Kuczek M.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 2

The lactate dehydrogenase catalysed reaction shows lag phase. This lag phase is easy for explanation if the consecution of first and second order equilibrium reactions were assumed for calculation of pyruvate trace concentration. The same explanation was accepted for calculation of significant pyruvate concentration. For calculation significant pyruvate concentration the consecution of simple Michaelis - Menten type and second order reactions was assumed. The exact solution of reaction rate equations system for this complex reaction was counted.

3

Changes in creatine kinase and lactate dehydrogenase activity, and in myoglobin concentration during strength efforts

88%

Slowinska M., Stefaniak T., Sobiech K. A.

Polish Journal of Environmental Studies

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1998

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tom 07

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nr 6

4

Cystathionine gamma-lyase [EC 4.4.1.1]: an enzymatic assay of alpha-ketobutyrate using lactate dehydrogenase

75%

Czubak J., Wrobel M., Jurkowska H.

Acta Biologica Cracoviensia. Series Zoologia

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2002

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tom 44

5

Regulating the influence of NAD, ATP, and Mgplus2 on the interaction of lactate dehydrogenase [LDH] from Lactobacillus leischmannii with cardiolipin bilayers

75%

Terlecki G., Rogozik K., Czapinska E., Gutowicz J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

6

Hypoxic induction of alcohol and lactate dehydrogenases in lupine seedlings

75%

Garnczarska M.

Acta Physiologiae Plantarum

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2002

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tom 24

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nr 3

7

Further evidence for the importance of lipid bilayers in the interaction between lactate dehydrogenase and phosphatidylserine

75%

Terlecki G., Gutowicz J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

Lactate dehydrogenase (LDH) is one of the glycolytic enzymes, which have been proved to have the capability to reverse non-specific adsorption on cellular membranous structures in vitro, as well as on the structural proteins of the contractile system of muscle cells. It has been suggested that this binding may play a physiological role, as it alters the enzyme’s kinetic properties. Our previous studies on this enzyme showed that its interaction with some anionic phospholipids reveals similar characteristics and similar effect on the activity of the enzyme to those wich had been observed for the interaction with membranous structures. Disruption of the lipid bilayers by nonionic detergent (Tween 20) restored the enzyme activity inhibited by the presence of phosphatidylserine (PS) liposomes. In this study, we used the measurement of enzyme tryptophanyl fluorescence spectra to monitor the interaction and possible changes in the enzyme conformation. The investigation provided further evidence of the importance of the bilayer structure in this interaction. Similarly to the effect on the activity of the enzyme, the addition of Tween 20 diminishes the quenching of the LDH tryptophanyl ﬂuorescence, and finally completely restores the fluorescence.

8

Comparison of prostaglandin and cimetidine in protection of isolated gastric glands against indomethacin injury

63%

Brzozowski T., Tarnawski A., Hollander D., Sekhon S., Krause W. J., Gergely H.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 5

75-88

Prostaglandins can protect the in vivo gastric mucosa against necrosis produced by a variety noxious agents. Cimetidine has also been shown to have protective properties in humans and in some models of experimental injury. Whether prostaglandins or cimetidine may protect gastric mucosal cells directly in the absence of systemic factors remains controversial. In the present study, the potential protective actions of prostaglandin and cimetidine against indomethacin injury were assessed in isolated rat gastric glands. Gastric glands were pre-incubated in oxygenated medium with either placebo, 16,16 dimethyl prostaglandin E2 (dm PGE2) or cimetidine and incubated at 37°C in medium containing 0.5 mg/ml of indomethacin for 2, 4 and 6 hrs. Cell injury and protection was assessed by the Fast Green exclusion test (viability test), leakage of lactate dehydrogenase (LDH) into the medium, and by scanning and transmission electron microscopy. In addition, the generation of PGE2 by the gland cells was determined using RIA assay. Indomethacin by itself significantly reduced the viability of gastric glands, increased LDH release into the medium and produced prominent ultrastructural damage. In contrast to cimetidine, co-incubation of gastric glands with dm PGE2 added to indomethacin, significantly reduced indomethacin-induced injury, increased the number of viable cells, reduced LDH leakage and diminished the extent of ultrastructural damage. The dose of indomethacin (5 µg/ml) which significantly inhibited the generation of PGE2 (up to 90% inhibition) had no effect on cell viability nor LDH release. We conclude that 1) exogenous PGE2 exerts a potent protective activity in vitro which is independent on neural, vascular and hormonal factors; 2) inhibition of endogenous PGs may not the primary mechanism in the deleterious action of indomethacin against damage to gastric glandular cells and 3) indomethacin can exert a direct cytotoxic effect on the mucosal cells in gastric glands.

9

Excitatory neurosteroids attenuate apoptotic and excitotoxic cell death in primary cortical neurons

63%

Leskiewicz M., Jantas D., Budziszewska B., Lason W.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 3

457-475

Some neurosteroids show neuroprotective action in in vitro and in vivo studies, but their interaction with apoptotic/necrotic processes has been only partially unraveled. The aim of the present study was to examine the effect of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEAS), pregnenolone (PGL) and allopregnanolone (Allo) on staurosporine-, glutamate-, and NMDA-induced damage in primary cortical neuronal culture. DHEA, DHEAS and PGL (0.1 and 1 µM) inhibited the staurosporine-evoked LDH release and decreased the number of apoptotic cells as shown by Hoechst`s staining, whereas Allo was without effect. The neurosteroids affected neither the staurosporine-evoked changes in caspase-3 activity nor the decrease in mitochondrial membrane potential. It was also shown that protective effects of DHEA, DHEAS and PGL against staurosporine-induced LDH release were attenuated by extracellular signal-regulated kinase (ERK) - mitogen-activated protein kinase (MAPK) inhibitor – PD 98059 (5 µM) but not by phosphatidylinositol-3-kinase (PI3-K) inhibitors such as LY 294002 (1 µM) or wortmannin (10 nM). The involvement of ERK2-MAPK in protective effects of neurosteroids was confirmed by Western blot study. Further study demonstrated that glutamate-induced cell damage was attenuated by DHEA, DHEAS, and PGL, but not by Allo. None of the steroids influenced NMDA-induced LDH release. The results of the present in vitro studies suggest that excitatory neurosteroids DHEA, DHEAS and PGL at physiological concentrations participate in the inhibition of cortical neuronal degeneration elicited by staurosporine and glutamate, whereas the most potent positive modulator of GABAA receptor - Allo - has no effect. Moreover, neurosteroids appear to attenuate the staurosporine-induced cell damage in a caspase-3 independent way and their neuroprotective mechanism of action involves the increase in ERK-MAPK phosphorylation.

10

The patterns of MHC association in aplastic and non-aplastic paroxysmal nocturnal hemoglobinuria

63%

Nowak J., Mika-Witkowska R., Mendek-Czajkowska E., Rogatko-Koros M., Graczyk-Pol E., Pyl H., Klimczak A., Wojcik M., Prochorec-Sobieszek M., Maryniak R., Zupanska B.

Archivum Immunologiae et Therapiae Experimentalis

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2011

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tom 59

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nr 3

11

Activity of LDH isoenzymes in diarrhoeic calves

63%

Sobiech P., Kuleta Z.

Bulletin of the Veterinary Institute in Puławy

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2006

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tom 50

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nr 3

12

The role of lipid phase structure in the interaction of lactate dehydrogenase with phosphatidylserine. Activity studies

63%

Terlecki G., Czapinska E., Gutowicz J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 3

Lactate dehydrogenase is one of the enzymes of the glycolytic path. It has been shown to be able to bind in vitro to cellular membranes. The presence of anionic phospholipids induces changes in the catalytic properties of the enzyme similar to those found when the enzyme is bound to natural membranes. In this study, a nonionic detergent (Tween 20), at concentrations not affecting the catalytic activity of LDH, was used to study the role of the lipid supra-molecular structure in the interaction between pig skeletal muscle lactate dehydrogenase and phosphatidylserine. Tween 20 changes the equilibrium of concentrations between the lipid supra-molecular forms. The detergent at the used concentration values did not alter the activity of the enzyme when it was used on its own, but did diminish the level of inhibition induced by the studied phospholipid. The obtained results showed that the interaction is reversible and that the bilayer structure of the lipid is essential for the inhibition.

13

Alterations of LDH and its isoenzyme activity in blood plasma of ewe lambs after exposure to chlorine in drinking water

63%

Sutiakova I., Sutiak V., Krajnicakova M., Bekeova E.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

|

nr 1

14

The effect of some epsilon-aminocaproic acid derivatives on platelet responses

63%

Bruzgo I., Tomasiak M., Stelmach H., Midura-Nowaczek K.

Acta Biochimica Polonica

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2004

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tom 51

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nr 1

ε-Aminocaproic acid (EACA) is a synthetic low molecular drug with antifibrinolytic activity. However, treatment with this drug can be incidentally associated with an increased thrombotic tendency. The aim of the present work was to test synthetic EACA derivatives for their antiplatelet activities. We investigated the effect of three EACA derivatives with antifibrinolytic activity: I. ε-aminocaproyl-L-leucine hydrochloride (HCl*H-EACA-L-Leu-OH), II. ε-aminocaproyl-L-(S-benzyl)-cysteine hydrochloride (HCl*H-EACA-L-Cys(S-Bzl)-OH) and III. ε-aminocaproyl-L-norleucine (H-EACA-L-Nle-OH) on platelet responses (aggregation and adhesion) and on their integrity. It was found that: 1. as judged by LDH release test, none of the tested compounds, up to 20 mM, was toxic to platelets, 2. in comparison with EACA, all the synthetic derivatives inhibited much stronger the ADP- and collagen-induced aggregation of platelets suspended in plasma (platelet rich plasma) and aggregation of these cells in whole blood, 3. EACA and its derivatives exerted a similar inhibitory effect on the thrombin-induced adhesion of platelets to fibrinogen-coated surfaces. Since platelet activation and blood coagulation are tightly associated processes, the antiplatelet properties of EACA derivatives are expected to indicate reduced throm- botic properties of these derivatives compared to EACA.

15

Serum LDH isoenzyme activity in dairy and beef cows

63%

Sobiech P., Kuleta Z., Jalynski M.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2002

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tom 01

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nr 2

16

Levetiracetam protects hippocampal neurons in culture against hypoxia-induced injury

51%

Sendrowski K., Bockowski L., Sobaniec W., Ilendo E., Jaworowska B., Smigielska-Kuzia J.

Folia Histochemica et Cytobiologica

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2011

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tom 49

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nr 1

17

Antioxidant enzyme activities and lipid peroxidation in Mytilus galloprovincialis from the French Mediterranean coast

51%

Gwozdzinski K., Gonciarz M., Kilanczyk E., Kowalczyk A., Pieniazek A., Brichon G.

Oceanological and Hydrobiological Studies

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2010

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tom 39

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nr 4

18

Secretion of proinflammatory cytokines by normal human melanocytes in response to lipopolysaccharide

51%

Tam I., Stepien K.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

A large body of evidence suggests that epidermal melanocytes are an integral part of the skin immune system and can be considered immunocompetent cells. Recently, it has been reported that human melanocytes constitutively express Toll-like receptors and may be involved in the induction of several inflammatory cytokines. In the study the secretion of IL-1β, IL-6 and TNF-α by cultured normal melanocytes was investigated after stimulation with lipopolysaccharide. LPS increased the secretion of IL-1β in a dose-dependent manner. IL-1β stimulated release of IL-6 and TNF-α by melanocytes, whereas LPS activated production of TNF-α, but not of IL-6. These observations indicate that LPS can participate in the regulation of cytokine activity in normal human melanocytes and suggest that cytokines released by melanocytes could affect melanocytes themselves or/and other cells of the epidermis.

19

Synergistic effects of amyloid peptides and lead on human neuroblastoma cells

51%

Suresh C., Johnson J., Mohan R., Chetty C. S.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

Aggregated amyloid peptides (AP), major components of senile plaques, have been considered to play a very important and crucial role in the development and neuro-pathogenesis of Alzheimer’s disease (AD). In the present in vitro, study the synergistic effects of Pb2+, a heavy metal, and AP on the human neuroblastoma SH-SY5Y cells were investigated. The cells treated with Pb2+ (0.01–10 μM) alone exhibited a significant decrease in viability and IC50 was 5 μM. A similar decrease in viability was also observed when the cells were exposed to AP, Aβ1–40 (20–120 μM) and Aβ25-35 (2.5–15 μM) for 48 hrs. The IC50 values were 60 μM and 7.5 μM for Aβ1–40 and Aβ25–35 respectively. To assess the synergistic effects the cells were exposed to IC50 of both AP and Pb2+, which resulted in further reduction of the viability. The study was extended to determine the lactate dehydrogenase (LDH) release to assess the cytotoxic effects, 8-isoprostane for extent of oxidative damage, COX 1 and 2 for inflammation related changes, p53 protein for DNA damage and protein kinases A and C for signal transduction. The data suggest that the toxic effects of AP were most potent in the presence of Pb2+, resulting in an aggravated clinical pathological condition. This could be attributed to the oxidative stress, inflammation neuronal apoptosis and an alteration in the activities of the signaling enzymes.

20

Cytochemical study of dehydrogenase activity in two euglenid species of the genus Parastasia Michajlow,1966

51%

Wita I.

Acta Protozoologica

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1989

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tom 28

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nr 1

Ograniczanie wyników

Alterations in the lactate dehydrogenase isoenzyme pattern after maximal running exercise

Simplified solution of lactate dehydrogenase catalysed reaction