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Studies were conducted on 8 cows and 22 heifers prepared as recipients of embryos. All the cows and 19 heifers were subjected to embryo transfers, the remaining 3 heifers being the control group. The temperature of the milk was taken automatically during milking and body temperature was measured rectally from the first day after synchronized oestrus up to 12 days following the embryo transfer. The heifers body temperature was taken rectally during the same period. Temperatures were recorded twice daily (in the morning and evening) and on the days of the embryo transfer they was recorded more often amongst the heifers. Following the embryo transfer, an increase in body and milk temperature was recorded in 6 cows, and in 12 heifers there was an increase in body temperature. In the decided majority of heifers (11) and in the majority of cows (4) an increase in body temperature occurred on the day of the embryo transfer. In the remaining animals this increase was recorded on the day following the embryo transfer. A probable reason for the increase in body and milk temperature in cows and body temperature in heifers, following embryo transfer, is considered to be an immunological response of the recipient's organism to the appearance of the embryo in the uterus.
The aim of this study was determining influence of some factors on pregnancy results in recipient heifers of fresh embryos. The analysis was carried out on 1641 animals (1847 embryo transfer interventions of an average age of 16-26 months. The mean pregnancy rate in heifers was 46.4% and was dependent (p<0.001) on which the quality of embryos, kind of medium used to embryo collection, herds from recipients descended, number of interventions on one recipient, stage of embryo development, kind of medium to embryo culture and conservation, horn of uterus and the month. The best results were noted in recipients which received very good than poor quality embryos (60 resp. 26.1%). There was a big difference in pregnancy results between recipients coming from different herds (23 resp. 60%). When heifers were used as recipients two or more times the pregnancy results were lower than in heifers used only once (47.1 resp. 43.6% and 19.2%). A significant influence on pregnancy results was also the kind of medium used for the cultivation of embryos (Dulbeco (Menezo) or Dulbeco (Sigma) - 50.2 or 56.6% resp. OCM (Imperial - 26.8%). Likewise results of pregnecies were highest in October (62.2%) and lower in summer months (July 33.3%).
The distribution and the number of the T. spiralis larvae per 1 g of muscle were determined in rats experimentally infested at the rate of 200, 500 or fed meat containing the larvae. The examinations were also carried out on pigs fed 200, 1000 and 10 000 larvae. The intensity of larvae invasion and distribution depended on the dose used for infestation. The highest number of the larvae in rats and pigs infested with lower doses of T. spiralis was found in the tongue muscles, masseter, femur and diaphragm. In animals infested with medium-sized inocula (rats with 500 and pigs 1000 larvae) the highest number of the larvae was noted in the intercostal muscles, in the masseter in the transversal muscles of the abdomen, in the biceps muscle of the thingh and in the diaphragm. In pigs infested with 10 000 larvae and in rats fed infested meat the highest number of the T. spiralis larvae was found in the diaphragm. Numerous larvae were also noted in the tails and oesophagi of the animals under study.
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