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Koniugat CENTOCOR FITC w diagnostyce wscieklizny. II. Badania zwierzat naturalnie zakazonych

100%

Zmudzinski J. F., Dabrowska A., Sadowski A., Klimczak Z., Smreczak M.

Medycyna Weterynaryjna

1993

tom 49

nr 02

74-75

There were compared the findings concerning diagnosis of rabies using anti-rabies conjugates, i.e. Czechoslovakian policlonal Bioveta and American monoclonal CENTOCOR. There were evaluated the impression slides stained with Bioveta and CENTOCOR conjugates obtained from 27 foxes, a racoon dog, a stone marten, 4 cats, 2 dogs and a cow. The animals were suspected for rabies. Out of 36 animals rabies was diagnosed in 32 cases with the Bioveta conjugate (88.9%) and only 18 with CENTOCOR (50%). The samples responding positive with the Bioveta and negative with CENTOCOR were assessed by inoculation of mice. The results suggested that the serotypes of rabies virus which were not diagnosed with CENTOCOR FITC could circulate in nature. Thus the CENTOCOR FITC conjugate is of a limited value for diagnostic purposes of rabies.

Koniugat CENTOCOR FITC w diagnostyce wscieklizny. I. Badania myszek zakazonych szczepem Flury LEP

100%

Zmudzinski J. F., Smreczak M., Skulmowska-Kryszkowska D., Rola J.

Medycyna Weterynaryjna

1993

tom 49

nr 01

19-21

The monoclonal CENTOCOR FITC conjugate produced by CENTOCOR, Malvern PA 19355, USA has been used for detection of rabies virus in brain impression slides from mice inoculated with Flury LEP strain MICLD₅₀ titer log 5,32. The conjugate has been used at the dilutions 1:10, 1:20, 1:40. For comparison same brain impression slides have been treated with anti-rabies policlonal conjugate made by czechoslovakian company Bioveta (working dilution 1:8). The control slides have comprised the impressions of normal (non-inoculated with rabies) mouse brain labeled with either CENTOCOR undiluted or Bioveta conjugate diluted 1:8. The same complexes emiting bright apple green fluorescence have been found out in impressions labeled with either Centocor or Bioveta conjugates. The CENTOCOR has given the positive reaction in the dilution up to 1:20. The background staining has been markedly reduced in impressions treated with CENTOCOR (contains Evans blue counterstain). The CENTOCOR lot No. 9C0011 may be used in the dilution 1:2 to provide 10-fold excess of antibody recommended by the company.

Ograniczanie wyników

2 Medycyna Weterynaryjna

2 Smreczak M.

2 Zmudzinski J. F.

1 Dabrowska A.

1 Klimczak Z.

1 Rola J.

1 Sadowski A.

1 Skulmowska-Kryszkowska D.

2 1993

Wyniki wyszukiwania

Koniugat CENTOCOR FITC w diagnostyce wscieklizny. II. Badania zwierzat naturalnie zakazonych

Koniugat CENTOCOR FITC w diagnostyce wscieklizny. I. Badania myszek zakazonych szczepem Flury LEP