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1

Zdolność oocytów klaczy do dojrzewania i zapłodnienia in vitro

100%
Mlodawska W.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 01
The aim of the review was to present current views on the factors influencing the in vitro maturation (IVM) and fertilization (IVF) of mare oocytes. The first two foals produced with the use of the so-called standard IVF (co-incubation of oocytes and spermatozoa in culture media) were born over 20 years ago. To date, it has been possible to obtain offspring in horses after the fertilization of in vitro matured oocytes by the intracytoplasmic sperm injection technique (ICSI) or by the surgical transfer of oocytes to the oviducts of inseminated mares (fertilization in vivo). Causes of the low efficiency of IVF in horses are complex and may be related to an incomplete maturation of oocytes, an inappropriate method of sperm capacitation in vitro, as well as the use of non-compliant media for the development of inseminated oocytes and/or early embryos. The paper describes the method of oocyte collection from mare ovaries and the most important factors influencing the number and quality of oocytes obtained. It discusses the relationship between the physiological status of ovarian follicles, cumulus oophorus morphology and the capacity of mare oocytes for in vitro maturation and fertilization. In addition, some aspects of nuclear and cytoplasmic maturation of equine oocytes are presented. The understanding of the in vivo maturation mechanisms of equine gametes and of the developmental requirements of embryos helps to improve culture conditions and the in vitro fertilization efficiency of equine oocytes.
2

Wpływ komórek ziarnistych i pożywek hodowlanych na kompetencje mejotyczne oocytów bydła oraz ich zdolność do rozwoju po zapłodnieniu in vitro

100%
Mlodawska W., Ploszaj E.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 05
The aim of the study was to compare the effect of gonadotropic hormones and granulosa cells on the maturation and developmental capacity of cattle oocytes in vitro, as well as the effect of TCM 199 and DMEM/F12 media on the development of embryos obtained in co-culture with oviduct epithelial cells. Fertilization was performed with the use of frozen semen from 2 bulls. Twenty hours after insemination, presumptive zygotes were placed in co-culture with oviduct cells in a TCM 199 (TCM-KJ co-culture) or a DMEM/F12 medium (DMEM-KJ co-culture) and cultured for 7-9 days. Metaphase II was reached by 40% and 48% of oocytes cultured in the presence of granulosa cells and gonadotropins, respectively. Only embryos obtained from oocytes maturing in the presence of granulosa cells developed to the blastocyst stage. Considerably more dividing embryos were obtained when the presumptive zygotes were co-cultured with TCM-KJ (38.1%) rather than with DMEM-KJ (8.6%; P < 0.01). This study showed that the presence of granulosa cells had no effect on the nuclear maturation of cattle oocytes, but increased their capacity for embryonic development. TCM 199 is much more useful than DMEM/F12 for the co-culture of cattle embryos with oviduct cells.
3

Systemy oceny kompetencji rozwojowej oocytów oraz zarodków ssaków oparte na technologii mikrofluidycznej typu Lab-on-Chip

100%
Kempisty B., Walczak R., Antosik P., Szczepanska P., Wozna M., Bukowska D., Zaorska K., Dziuban J., Jaskowski J.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 01
This review presents basic criteria for evaluating the developmental competence of oocytes and embryos, and contains a detailed description of the microfluidic-technology-based Lab-on-Chip. The developmental competence of oocytes is acquired through a complex process associated with oocyte growth and maturation, the storage of large amounts of mRNA and proteins, and with the formation of proper cell morphology. The full maturation of oocytes is required for successful monospermic fertilization and embryonic preimplantation development. The morphology of the gamete is one of the most important factors influencing the developmental competence of the cell. There are several indicators for the assessment of oocyte morphology, most of them including the color and granularity of the cytoplasm. Intensive research is under way to develop and introduce new non-invasive methods of oocyte and embryo quality assessment as a major factor in the improvement of assisted reproductive techniques. The Lab-on-Chip technology, as an independent micro-cytometric device, is a combination of reproductive biology techniques and micro-optic electronics. In the future, Lab-on-Chip systems may be used as an important diagnostic instrument for evaluating the quality of mammalian oocytes and embryos.
4

Przydatność oceny konfiguracji chromatyny oocytów ssaczych, jako markera kompetencji mejotycznych i rozwojowych

100%
Gasior L., Daszkiewicz R.
Postępy Biologii Komórki
|
2014
|
tom 41
|
nr 4
5

Zastosowanie mikrochipu fluidycznego typu Lab-on-Chip do oceny jakości oocytów i zarodków ssaków

100%
Kempisty B., Walczak R., Sniadek P., Piotrowska H., Wozna M., Jackowska M., Bukowska D., Antosik P., Dziuban J., Jaskowski J. M.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 08
The developmental competence of oocytes and embryos is one of the basic factors in determining whether the oocytes will be able to grow and mature and whether the embryos will be able to develop in the preimplantation stage and implant properly. There are several methods of evaluating the developmental competence of oocytes and embryos. The most frequently used include the oocyte maturity test using brilliant cresyl blue (BCB), and molecular analyses, such as genomics, transcriptomics, and proteomics. The Lab-on-Chip (LOC) system is a microcytometer that analyzes the spectrum of light absorption. The analysis of oocyte and embryo competence with this system concerns the thickness and color of the zona pellucida, as well as the color and granularity of the cytoplasm. The microfluidic chip includes two micro fibers and two channels: one leading in and one leading out. The total measuring system comprises a docking station with a chip, micro-spectrophotometer, and laptop. The system can therefore be used under non-laboratory conditions. Analysis with this system is a new and noninvasive method of bovine and porcine oocyte and embryo quality assessment, and may in the future be one of the main tools used in evaluating the developmental competence of these cells. This article presents the possible function and use of a system based on the microfluidic technology. Moreover, the construction and applications of the LOC are discussed, including the possibilities of LOC application in analyses of the developmental competence of bovine and porcine oocytes and embryos.
6

Ocena kompetencji rozwojowych zarodków króliczych po zapłodnieniu in vivo w różnych stadiach rozwojowych

100%
Kij B., Checko A., Fryc K., Nowak A., Kozlowska A., Kochan J.
Roczniki Naukowe Zootechniki
|
2018
|
tom 45
|
nr 2
7

Czynniki warunkujące potencjał rozwojowy oocytów ssaków – w świetle badań molekularnych i mikrofluidycznych

100%
Kempisty B., Piotrowska H., Walczak R., Sniadek P., Dziuban J., Bukowska D., Antosik P., Jackowska M., Wozna M., Jaskowski J. M.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 07
Several morphological, molecular and cellular changes lead to the differentiation of primordial germ cells (PGC) into gametes, eggs and spermatozoa. This process is followed by the migration of these cells to gonads and several cell division cycles (mitotic and meiotic) as well as cell differentiations where these cells are changed into fully mature gametes. The process of gametes maturation is composed of several stages of specific biochemical changes that include changes in the nucleus as well as the changes in oocyte’s cytoplasm. The main factor which determines the formation of the developmental competence of oocytes is the long stage of mRNA and proteins storage (cytoplasmic maturation) that plays the main role in the blastocyst formation process. In this article selected issues associated with the regulation of each of the stages of oocytes differentiation as well as their influence of selected factors such as follicular size or formation of oocyte’s transcriptome have been presented. Moreover, a new-noninvasive system of oocytes/embryos quality assessment by using microfluidic techniques was presented.
8

Ocena kompetencji rozwojowej oocytów oraz zarodków świń na podstawie badań molekularnych i mikrofluidycznych

80%
Kempisty B., Walczak R., Sniadek P., Dziuban J., Piotrowska H., Zawierucha P., Zaorska K., Antosik P., Bukowska D., Jaskowski J. M., Nowicki M., Zabel M.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 10
Evaluation of oocyte developmental competence has an important influence on the ability of these cells to attain maturation, successful fertilization, development of embryo to the blastocyst stage and proper implantation. Factors determining the reproductive potential of gametes included: (1) expression of important transcription factors, (2) epigenetic changes, which influence the silencing of selected genes transcription, (3) transcription regulation, and (4) post-transcriptional regulation. The epigenetic changes mainly include: DNA methylation, histones modifications and changes in chromatin structure in the oocytes. In several studies, the association between oocyte morphology (mostly determined by cumulus cell layers and granularity of cytoplasm) and the ability of these cells to attain maturation and fertilization has been described. The use of biochemical, metabolomical and molecular markers is the most frequently applied tool in the assessment of oocyte developmental competence. However, most of these methods are invasive and lead to the decreased viability of the analyzed cells. Searching for new, objective and noninvasive techniques leads to the development of a microfluidic chip system, which shows the physical (spectral) properties of oocytes and embryos in comparison to the biological parameters. In this article, selected issues associated with the genetic regulation of such processes as: maturation of oocytes, fertilization and early stages of embryonic development, have been presented. Moreover, the regulation of transcription and post-transcriptional modification during oogenesis and embryogenesis in mammals, with special relation to pigs and the possibilities of applying of microfluidics in assessment of oocyte and embryo developmental competence was shown.
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