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Juvenile of Trichogaster lalius was reared under three photoperiod conditions (0L:24D, 12L:12D, 16L:08D) for 60 days to examine the growth performance under laboratory conditions. The maximum growth was observed in the juvenile of T. lalius exposed to 16:00 hrs. light. The mean body weight of different groups of juvenile fish exposed to different light conditions were significantly different (p<0.05) from each other which was observed from 20thday of the experiment. The absolute, specific, and relative growth rates were found to be maximum in the group exposed to 16:00 hrs. light duration. The present study indicated that 16:00 hrs. light duration was considered to be better for the growth of juvenile of T. lalius under controlled condition.
The infection of metacercariae of Posthodiplostomum cuticola (v. Nordmann, 1832), the agent of black-spot disease, was investigated in 0+juvenile fish from 19 localities in south-east Moravia, Czech Republic. Of the 30 fish species examined, only cyprinids of 12 species were found to be infected. Five species of Leuciscinae, Rutilus rutilus, Leuciscus idus, Scardinius erythrophthalmus, Abramis bjoerkna and Abramis brama, fish with similar behaviour and habitat preference in juvenile period, were the most susceptible fish hosts for P. cuticola metacercariae. Especially high infection parameters were found in intergeneric hybrids of the studied leuciscine species. Environmental conditions were an important factor for parasite infection. The fish from flooded borrow pits, inhabiting lentic water bodies with dense vegetation in the littoral zone, showed high prevalence and abundance of P. cuticola infection contrary to their conspecifics in water reservoirs with steep banks and fish from rivers.
Gyrodactylus infections in intensively-reared populations of Nile tilapia, Oreochromis niloticus niloticus, have been associated world-wide with high mortalities of juvenile fish. In this study, 26 populations of Gyrodactylus parasitising either O. n. niloticus or Mozambique tilapia, Oreochromis mossambicus, were sampled from fourteen countries and compared with type material of Gyrodactylus cichlidarum Paperna, 1968, Gyrodactylus niloticus (syn. of G. cichlidarum) and Gyrodactylus shariffi Cone, Arthur et Bondad-Reantaso, 1995. Representative specimens from each population were bisected, each half being used for morphological and molecular analyses. Principal component analyses (PCA) identified five distinct clusters: (1) a cluster representing G. cichlidarum collected from O. n. niloticus from 13 countries; (2) the G. shariffi paratype; (3) three specimens with pronounced ventral bar processes collected from two populations of Mexican O. n. niloticus (Gyrodactylus sp. 1); (4) four specimens collected from an Ethiopian population nominally identified as O. n. niloticus (Gyrodactylus sp. 2); (5) nine gyrodactylids from South African O. mossambicus (Gyrodactylus sp. 3). Molecular analyses comparing the sequence of the ribosomal transcribed spacer regions (ITS 1 and 2) and the 5.8S gene from the non-hook bearing half of worms representative for each population and for each cluster of parasites, confirmed the presence of G. cichlidarum in most samples analysed. Molecular data also confirmed that the DNA sequence of Gyrodactylus sp. 2 and Gyrodactylus sp. 3 (the morphologically-cryptic group of South African specimens from O. mossambicus) differed from that of G. cichlidarum and therefore represent new species; no sequences were obtained from Gyrodactylus sp. 1. The current study demonstrates that G. cichlidarum is the dominant species infecting O. n. niloticus, being found in 13 of the 15 countries sampled.
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