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Propagation of juniper from cuttings is the primary method used in the nursery. Process of rhizogenesis in these plants is long and not always allows to obtain a good quality of rooted cuttings. In the experiment there was investigated the effect of laser light doses (semiconductor laser, model CTL 1106MX, power 200 mW, wave length 670 μm) and the footing agent AB on percentage of rooted cuttings, plants height, length and the number of roots. The examined juniper taxa proved diverse response to the variants applied (combination of laser radiation and the rooting agent AB). The increase in the number of rooted cuttings after application of the rooting agent AB, as well as three times introduced irradiation in Eastern redcedar ‘Blue Arrow’. This form also produced longer roots for the variant combining twelve times applied irradiation and the effect of rooting agent. In the cuttings of Chinese juniper ‘Stricta’, exposed to laser radiation in six – (D6) and twelve times applied doses (D12), there was observed significant stimulation of root length. Introduced treatment variants of shoot cuttings of Common juniper ‘Schneverdinger Goldmachangel’ brought about considerable root elongation (except for D3 dose). The dose providing for significant increase in the percentage of rooted cuttings in all the examined juniper varieties proved to be D3 dose.
Juniperus communis L. is increasingly threatened in many parts of Europe, including the Baltic region. Our present study was aimed at evaluation of genetic diversity of J. communis populations of Lithuania. Fourteen selected populations differed in geography and habitats (coastal brown dunes covered with natural Scots pine forests, further referred as B; J. communis shrubs, F; transition mires and quaking bogs, D; subcontinental moss Scots pine forests, G; xero-thermophile fringes, E). Molecular variance was analyzed among populations (140 individuals, in total), employing 14 Inter Simple Sequence Repeat (ISSR) markers. Percentage of polymorphic loci differed between populations belonging to the different habitat type, being the highest for population representing habitat F (68.2%) and the lowest for populations D (42.9%). The data demonstrate the possibility to underestimate or overestimate this parameter if some habitat populations are bypassed. Means per population of Nei‘s gene diversity and Shannon‘s information indexes, were 0.158 and 0.239, respectively. Significant correlation between genetic and geographic distance of populations of J. communis was documented by Mantel test. Bayesian analysis of ISSR data has separated populations of Southern Lithuania from Northern part. Juniperus communis populations representing different habitats showed moderate interpopulation variance at ISSR loci. In ISSR-based dendrograms, individuals were correctly allocated to populations, even in case of populations growing in 0.5 km vicinity (habitats D and G). Among all populations the most distinct one was representing habitat of J. communis shrubs (F) protected by EUNIS. Principal coordinate analysis of weighted averages of Ellenberg’s indicator values (EIV) for herbaceous species, separated populations according to habitat type, while the same type analysis of ISSR data allowed distinguishing E habitat populations from populations of all other habitat type but G. Our study shows that on the local (Lithuanian) scale, molecular variance between populations of J. communis at ISSR loci might be related to habitat type. For evaluation and preservation of diversity of J. communis, different assessment methods should be included and management strategies should be directed in the way to retain variety of habitats encompassing both widely spread and less common ones.
Common juniper (Juniperus communis L.) is the well-known and valuable medicinal plant. Juniper berry is used as a herbal medicine mainly due to its essential oil with diuretic, antiinflammatory, choleretic and cholagogue activities. Some other substances, as polyphenols, are present in juniper berries and other parts of this plant. The aim of this study was to estimate total polyphenols content in common juniper sprouts (leaves) originating from different places at the kujawsko-pomorskie province. The total polyphenols content was carried out by means of spectroscopic method with Folin-Ciocalteu reagent and shown as pyrogallol equivalents. The obtained results ranged in limits from 2.40±0.23% to 3.43±0.17%.
The observed juniper decline, lack of natural regeneration, and disappearance of numerous populations of the common juniper in Poland, were the major reasons for initiation of research on its sexual propagation and seedling production. This study shows that seed dormancy in this species is broken by warm-cold stratification at 15°C/3°C, for 14+12 weeks, respectively. Seed drying for 72 h at room temperature in the middle of the warm phase (i.e. after 4–8 weeks of stratification at 15°C) and the following cold stratification at 3°C, cause a significant increase in seed germination capacity at cyclically alternating temperatures of 3~15°C, and in seedling emergence in growing trays (67 cells each) in a greenhouse. However, seedling emergence was over 2-fold to 4-fold lower in an open nursery than in the greenhouse. After sowing in the nursery, secondary dormancy was probably induced, because some seeds germinated in the following year. Some very young, 2-year-old seedlings started to produce male or rarely female cones. This study also showed that soil conditions of mother plants can influence the pattern of seed germination and seed ling emergence.
The aim of the present study was biometrical comparison of three Iberian populations of Juniperus phoenicea, represented by the subsp. turbinata and subsp. phoenicea. Eight features of the cones and seeds, two of the shoots and leaves were studied. The biometrical analysis of three distant populations of J. phoenicea shows great taxonomic distances among them. Two of them, representatives of J. phoenicea subsp. turbinata, are closer related each other than to the third, which represents J. phoenicea subsp. phoenicea. These results confirm the genetic differentiation of the taxons and also the biochemical and morphologic division of them. Nevertheless, the distances between particular populations are so great that more resemble the distances between species than between subspecies.
These studies were undertaken to identify the reasons for decay of spruce and juniper trees growing near the Metalplast plant in Oborniki. The material from the trees growing in this region was analyzed for the presence of free and complex-bound fluorine (FA) and total content of fluorine (FB), as well as some metals known to hae harmful effects on plants. The results were compared with analogous data obtained for plants growing in the area free from this kind of pollution, in Kórnik. The comparison has confirmed the hypothesis claiming that the main reason for decay of trees of the above species growing in the neighbourhood of the Metalplast plant in Oborniki is pollution by fluorine compounds.
The viability of base-treated juniper fiber (BTJF) media for removing toxic heavy metals (Cd2+, Cu2+, Pb2+, Zn2+) in stormwater runoff was investigated. The sorption ability of the BTJF for all metals was much higher than that of untreated juniper. The affinity sequence of both materials, BTJF and untreated juniper, was Pb > Cu > Zn > Cd. This order is explained by the hydrolysis constants for each metal. A metal desorp­tion and column regeneration test using 0.1 M nitric acid showed that the metal sorption capacity declined slightly from 136.3 to 119.2 ^mole/g in the first two cycles and then more significantly at the third and fourth regeneration, 72.3 and 83.1 ^mole/g, respectively. Based on the hydraulic conductivity test of BTJF of different size classes, it can be deduced that there is no major headloss-related disadvantage in using BTJF instead of sand as stormwater filter media if the particle size of the BTJF is similar to that for sand.
The aim of undertaken research was recognition the reasons of dying out the common juniper Juniperus communis L. ssp. communis population in the forest nature reserve “Jałowce” (Pomerania). Applied methods of dendrochronological analysis let to determine the age of juniper-stand in the reserve and describe the influence of climatic conditions on its growth. Obtained results testify to Juniperus communis chronology with signature WIE collected from 17 individual sequences which numbered 102 tree ring width and represented a time span 1903 to 2004. Such results described the juniper-stand in the reserve as ageing population with the oldest specimen at age 98 years. However, the last several years were characterised by the lack of strong growth depressions typical for earlier time periods with tree ring width ranged 0.7–1.0 mm (themean annual tree ring width amounted to 0.85 mm), but the visible symptoms of degeneration and dying out of protected common juniper population intensified probably because of its age.
Four populations of J. communis L. subsp. communis of natural localities in forested regions of the Bory Tucholskie (Poland) were analysed in respect of phenolic compounds concentration and –glucosidase activity. The variability noted in needles was confirmed by adaptation changes; water-logged and poor insolated localities (deciduous mixed fresh forest, dmff – no 3 and coniferous mixed bog forest, cmbf – no 4) stimulated the phenolics accumulation and enzyme activity. On the contrary, the level of phenolics in unripe berry-like cones was almost two fold lower under disadvantageous juniper growth and fructification conditions, whereas the mature berries from poor vigour plants were characterized by higher concentrations of these metabolites. Obtained results show that morphological variation of juniper population was accompanied by variability in phenolic metabolism.
The intra- and inter-population variation in three populations of Juniperus excelsa, two from Crimea and one from the Balkan Peninsula, were analyzed biometrically. Fourteen morphological characters of cones, seeds, shoots and leaves were used. The number of seeds per cone appears to be the most variable character. The others were more stable. Differences among particular individuals within the samples were slight, as well as between populations compared. The Crimean samples were very close to each other, while the more geographically distant sample from the Balkan Peninsula appears also to be morphologically more separate. This suggests that the Balkan populations originated from another Pleistocene refugium. The Crimean populations did not show the reduction of variability, which could have resulted from their geographical isolation and their considerably restricted numbers of individuals.
Seasonal foods of European rabbits Oryctolagus cuniculus (Linnaeus, 1758) were studied by microhistological analysis of pellets in a protected area Bugac Juniper Forest, Hungary. Field experiments were also conducted to examine the role of rabbit foraging on common juniper Juniperus communis. The proportion of grasses, forbs and browses changed significantly throughout the seasons (p < 0.0001). Spring diet was dominated by grasses (89%), summer diet by grasses (30%) and forbs (42%), whereas in autumn grasses (60%) and browse (24%) were the main diet components. Juniper appeared only in winter diet (19%) together with other browse (60%). The high proportion of aromatic thyme Thymus glabrescens in summer diet (30%) and juniper in winter diet indicates that monoterpenoids of these plants did not keep rabbits from consumption even when other food sources were available. Planted juniper seedlings disappeared within weeks due to the browsing by rabbits.
The effect of light conditions on the accumulation of phenolic compounds was investigated in the needles of one-year and two-year old shoots, collected from perennial specimens of common yew (Taxus baccata L.), American arborvitae (Thuja occidentalis L.) and common juniper (Juniperus communis L.), growing in an urban garden. The content of total phenols and flavones was partially related to the light requirement or light tolerance of conifer tree. In Taxus needles, a shade-loving species, higher accumulation of phenolic metabolites was observed under shaded condition (in two-year old shoots) and was linked with the growth intensity in spring. In Thuja and particularly in Juniperus, the level of phenolics was related to the insolation intensity, probably so as a part of the adaptation mechanism.
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