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The aim of our study was to evaluate the genetic relationship of Rhododendrons from Rhododendron collection at Tomaszkowice in Pogórze Wielickie. Leaves and leaf buds of three evergreen Asiatic species: R. aureum, R. brachycarpum, R. purdomii, six cultivars from Catawbiense Hybridum group (R. catawbiense): Catharine van Tol, Nova Zembla, Album Novum, Boursoult, Old Port, Hachmann’s Charmant and one cultivar from Yakushimanum Hybridum group: Koichiro Wada were investigated. Two plants from each accession were analysed. DNA was isolated by Ziegenhagen and Scholz [1998] protocol, dedicated to the difficult species in terms of high quality DNA extraction. After isolation, DNA was amplified with 19 RAPD primers. We obtained 255 RAPD markers and worked out a dendrogram, which illustrated the level of genetic diversity among the tested plants. Our study confirmed a close relationship between rhododendrons from Catawbiense Hybridum group. Another group was constituted by R. aureum and R. purdomii. The third group consisted of R. brachycarpum and Koichiro Wada cv.
The aim of the presented studies was to evaluate the usefulness of previously described method for the diagnosis of green mold in Polish mushroom-growing cellars. The PCR analysis showed the possibility of its use for distinguish Trichoderma aggressivum strains, occurring in mushroom crops. Molecular differences among green mold isolates were stated. They showed DNA band pattern characteristic for T. aggressivum f. europeanum as well as different pattern, suggesting the presence of strains belonging to T. aggressivum f. aggressivum. We successfully amplified purified DNA as well as DNA from purified cells suspension and cells collected on the casing surface while were not able to obtain PCR product from compost and casing suspensions.
Pseudomonas tolaasii, the causual agent of brown blotch disease of cultivated mushrooms in mushroom-growing cellars, is the subject of presented study. Populations of these bacteria are not homogeneous. This work shows that even in the case of one mushroom-growing cellar populations of bacteria are complex (with at least 3 genetically different lines). PCR starters developed by Lee, with the use of nested PCR method allow for efficient identification of strains of P. tolaasii occurring in Polish mushroom farms. The method is an efficient way to distinguish the strains of P. tolaasii from other microorganisms commonly found in mushroom-growing cellars, such as V. fungicola and T. aggressivum. This is particularly important due to the fact that these organisms, in some cases, give similar symptoms of disease in mushroom crops (especially in early stages of disease development). Application of a fast and unambiguous method for pathogen identification can significantly contribute to the proper choice of disease control method.
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