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Biosynthesis of polyhydroxyalkanotes in wild type yeasts

100%

Abd-El-Haleem D. A. M.

Polish Journal of Microbiology

2009

tom 58

nr 1

37-41

Biosynthesis of biodegradable polymers polyhydroxyalkanotes (PHAs) have been studied extensively in wild type and genetically modified prokaryotic cells, however the content and structure of PHAs in wild type yeasts is not well documented. The purpose of this study was to screen yeast isolates collected from different ecosystems for their ability to accumulate PHAs. Identification of the isolates and characterization of PHAs produced by the positive isolates were investigated. One positive isolate (strain Y4) was identified by both API20C system and 18S rDNA sequencing. The data revealed that isolate Y4 belongs to the yeast genus Rhodotorula and exhibits 18S rDNA similarity value >99% to the species Rhodotorula minuta. Quantification of PHAs yield of strain Y4 in glucose, oleic acid and tween 60 containing medium for over a growth period of 96 h gave 2% of PHAs in biomass. The nature of produced PHAs was analyzed by infrared spectroscopy and nuclear magnetic resonance (¹H and ¹³C NMR) and found to contain polyhydroxybutyrate and polyhydroxyvalerate (PHBV).

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Preliminary study of endophytic fungi in timothy (Phleum pratense) in Estonia

100%

Varvas T., Kasekamp K., Kullman B.

Acta Mycologica

2013

tom 48

nr 1

Timothy (Phleum pratense L.) is an important agricultural grass in Europe and North America, but there is little research into the occurrence and abundance of fungal endophyte species associated with this grass. The aim of this study was to identify fungal endophytes living within P. pratense and to determine if additional moisture applied during the growing season increases the diversity of endophytic fungi. We studied 58 isolates obtained from surface-sterilised blades of 60 P. pratense plants collected from Rõka Free Air Humidity Manipulation experimental plots (FAHM), Estonia. Morphological and molecular methods were used for isolate identification. As a result, 45 strains from 10 different taxa were identified, all belonging to Ascomycota. Five species were found to be new to P. pratense.

Ograniczanie wyników

1 Acta Mycologica

1 Polish Journal of Microbiology

1 Abd-El-Haleem D. A. M.

1 Kasekamp K.

1 Kullman B.

1 Varvas T.

1 2013

1 2009

Wyniki wyszukiwania

Biosynthesis of polyhydroxyalkanotes in wild type yeasts

Preliminary study of endophytic fungi in timothy (Phleum pratense) in Estonia