Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 15

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  inwazja eksperymentalna
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Research concerning the distribution as well as intensity of Trichinella larvae in the bodies of hosts is crucial from the perspective of the invasionology and diagnostics of Trichinellosis. Diagnosing Trichinella spiralis is dependent on the following factors: intensity of Trichinella spiralis invasion in the muscles, the weight of the material, the specific types of muscles, animal species, as well as the method of testing for Trichinellosis presence. The goal of the authors’ study was determining the distribution and intensity of Trichinella spiralis larvae invasion in selected rabbit muscles experimentally infected with a small dose of Trichinella. The investigations were conducted on 8 healthy slaughter rabbits that weighed 5 kg. The rabbits were infected with a single dose of 90 Trichinella spiralis muscle invasive larvae derived from a boar. The rabbits were slaughtered 60 days after infection. Select entire muscles from the left and right sides of the carcasses were chosen for investigation. From rabbit the following muscles were tested for Trichinellosis presence: Diaphragma (part: lumbalis, costalis, sternalis), m. masseter, antebrachii, tongue, m. biceps brachii, pterygoideus, cruris, mm. intercostales, m. semitendinosus and semimembranosus, as well as m. longissimus lumborum. The distribution as well as intensity of the T. spiralis larvae invasion (trichinellosis count per 1 g of muscle, as well as percentage in relation to the diaphragm = 100%) in the investigated muscles was determined by the digested method according to Commission Directive (WE) no. 2075 as of December 5, 2005. All the rabbits used in the experiment were infected: encysted T. spiralis larvae were confirmed in all the examined muscles. The results of the conducted investigations showed statistically significant differences in the T. spiralis invasion of specific muscles. The greatest trichinellosis larvae invasion occurred in the m. masseter, antebrachii and in the diaphragm. The intensity of the T. spiralis invasion in the examined muscles was 18.92 to 1.20 per 1 g of muscle and was from 1.08 to 17.13 times less than the trichinellosis invasion in the m. masseter. These results indicate the high suitability of the m. masseter for investigating the presence of trichinellosis in rabbits. Rabbits are susceptible to T. spiralis infection. The trichinellosis larvae undergo encystment in their muscles. The muscle tissue of all rabbits infected with T. spiralis was brought on by rat trichinellosis.
The studies were carried out on guinea pig males weighting about 280 g. The animals were infected with 5000 or 20000 invasive eggs of Ascaris suum. On the 3-rd, 7-th, 10-th and 14-th day of invasion the animals were dissected. Lungs were weighted, and the number of Ascaris larvaes was estimated using Baermann method. The activity of alpha-amylase (Fennel's method) and trypsin (Anson's method) were examined in pancreas homogenate. The infection of guinea pigs with A. suum larvaes resulted in a decrease of amylase and trypsin activity in parcreas. The activities were the lowest at the 3-rd day of invasion for amylase and at the 7-th day for trypsin. The results obtained for the animals infected 5 or 20000 invasie eggs A. suum did not differ statically.
The activation of muscle cell nucleus in the course of T. spiralis infection, established using morphological methods (part I), has been confirmed in histochemical (histones, RNP) and histoenzymatic (RN-aze) investigations. The activity of the cell nucleus increased from the 5th day after infection up to the complete encapsulation of the larva (30th day) however it remained at a weak stable level in later stages of infection.
The objective of the study was to establish possibilities of the development of the I stage larvae of E. cervi in fishes and amphibians, and possibilities of paratheny in the organism of these animals. In the alimentary tract of the fishes both the I stage and the invasive (i.e. III stage) larvae can not cross the intestine barrier and undergo digestion. However, in the frag organism the I stage larvae of E. cervi are capable of crossing the wall of the alimentary canal and of going through a part of their development, though not attaining the II stage. The invasive larvae fed to the frogs penetrate under the peritoneum, into the mesenterium and the muscles and there they survive inside cysts. Frogs as parathenic hosts for E. cervi can be dangerous for predators feeding on amphibians. The larvae of E. cervi liberated in their organism can pass through the cerebral phase of their development thus causing neural clinical symptoms.
Single doses (from 300 to 1000 larvi per an animal) of invasive larvae E. cervi Cameron, 1931, obtained from experimentally infected snails Helix pomatia L. were given to 17 guinea pigs and 17 golden hamsters. Clinical nervous symptoms in the form of paresis and paralysis of limbs occurred only in the guinea pigs which were given a dose of 1000 larvi. These animals died in the period from the 75th to 117th day of infection. From their central nervous system single adult males and females of E. cervi were isolated. In the lungs and mesenteries of 2 dead pigs live larvae of E. cervi were found. This fact proves that the guinea pig can fulfil the role of a final and a paratenic host of E. cervi. No clinical symptoms were noticed in any hamster. In hamsters dissected on the 7th day of infection live larvae of E. cervi were found in the mesentery and in the fleshy part of the diaphgram. After 14 days the larvae found both in the mesentery and in the diaphragm were dead and surrounded by cellular infiltration. A strong tissue reaction of the hamster after the administration of E. cervi larvae is responsible for the larvae destruction and resorption.
The studies were carried out on 90 male guinea pigs ot which 75 were infected per os with a dose of ca. 5000 invasive eggs of Ascaris lumbricoides suis. At the 1st, 2nd, 4th, 7th and 20th day ot intection blood was taken from experimental and control animals. The activity of LDH and its liver traction (spectrophotometric method), GGTP (circle test method) was determined. The level of glucose (orthotoluidin methhod) and alpha amino nitrogen (ninhydrin method was determined. In the course of larval ascariasis an increase in the values of the studied parameters was noted, especially at the 4th and 7th day of infection. The changes in the activity of the studied enzymes evidence disturbances of the structure and function of the liver of infected animals. The increased level of glucose and alpha amino nitrogen is probably associated with a disturbance of basic energy metabolism of the host organism.
Peroxidase-antiperoxidase (PAP) method was used to detect Toxoplasma gondii in the central nervous system of experimentally infected mice. Due to its capability for accurate localization of a parasite antigen among the populations of host cells, the method proved very useful in the diagnosis as well as in observations on the development of pathological changes.
The percentage of NBT-positive cells in peritoneal fluid, spleen and mesenteric lymph node of CWF mice infected orally with 200 Trichinella spiralis larvae was investigated. The highest level of NBT-positive cells in peritoneal fluid was found on the 5-6th and 50-60th day post infection (p.i.), in spleen between the 30-75th and in mesenteric lymph node between the 50-75th day p.i. The lack of reaction between peritoneal fluid cells and newborn migrating larvae at the 15th and 20th day p.i. observed by the authors, may be associated with slight activity of these cells in the NBT reduction test between 8-30th day p.i.
Bauflo male rats were infected with Hymenolepis diminuta. On 7th, 14th, 21th, 28th and 52th day of infection blood and liver were collected to determine AACo, AspAt, AlAt in the blood serum and in liver homogenates. In the course of teniosis in rats the activitics of all examined enzymes show changes in the serum and in liver homogenates. The most pronounced ones occur between the 7th and 21st day of infection. In the authors' opinion this is related to the highest pathogenicity of H. diminuta and especially with toxic action at this time. Determination of AACo activity proved to be a useful test to follow up the dynamics of organ changes in the course of hymenolepidosis.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.