The subject of this study was thirty nine strains of Yersinia enterocolitica, isolated from faeces of humans who showed symptoms typical of intestinal yersiniosis, and seventy strains of Y. enterocolitica, four strains of Y. pseudotuberculosis, and one strain of Y. kristensenii from healthy pigs. In the population tested the following serogroups appeared: 03, 09, 02, 05. A PCR was used to detect the presence of pathogenic chromosomal markers, such as myfA and inv genes of the tested Yersinia species. Among Y. enterocolitica strains isolated from humans and belonging to serogroup 03 (thirty four strains) and serogroup 09 (five strains) thirty three Y. enterocolitica 03 strains and four Y. enterocolitica 09 strains, gave a positive reaction to the myfA gene, yielding a fragment of 280 base pairs (bp). Among seventy Y. enterocolitica strains isolated from pigs forty strains belonging to serogroup 03 and fifteen strains belonging to serogroup 09 gave a positive reaction to the myfA gene. The presence of 390 bp amplified products, corresponding to the inv gene fragment, was detected in PCR products of three Y. pseudotuberculosis strains from pigs and only in one Y enterocolitica 03 strain from humans, which had no myfA gene. The results obtained show that the myfA gene is only present in the strains that belong to pathogenic serotypes of Y. enterocolitica. The myfA gene prevailed in the Y. enterocolitica 03 and 09 strains from humans but was less common in the Y. enterocolitica 03 and 09 strains from pigs.
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