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1

Distribution of NADPH-diaphorase and nitric oxide synthase in porcine ovaries after intraovarian infusions of Escherichia coli endotoxin

100%
Jana B., Andronowska A., Kucharski J.
Polish Journal of Veterinary Sciences
|
2000
|
tom 03
|
nr 3
The aim of this study was to determine whether pathological changes in Escherichia coli endotoxin (LPS, lipopolysaccharide)-infused ovary are associated with an increase in NO production, and whether inflammatory mediators produced by LPS-infused ovary can affect the contralateral one to stimulate NO synthesis. Therefore, the activity of NADPH-diaphorase (NADPH-d) and distribution of the inducible isoform of nitric oxide synthase (iNOS) used as indicators of the intensity of the inflammatory process were studied in porcine ovaries after unilateral infusions of LPS into the hilus of one ovary. Fourteen sexually matured gilts with controlled estrous cycle were used. The animals were randomly divided into two groups: I (control; n=7), and II (treated; n=7). In the group I, 1ml of saline was infused into the hilus of each ovary, on the 15th to the 19th day of the estrous cycle, twice a day (at 06:00 and 18:00). The gilts of the II group received 2 mg of LPS (serotyp 055:B5) in 1 ml of saline infused into the hilus of one ovary on the same days of the estrous cycle. At the same time, 1 ml of saline was infused into the contralateral ovary. The ovaries were collected on the 7th day of the next estrous cycle during a median laparotomy. Cryostat sections of the paraformaldehyde fixed ovarian tissues were stained histochemically to study the activity of NADPH-d and immunohistochemi- cally to investigate the distribution of iNOS. In the control group, the activity of NADPH-d was similar either in the corpora lutea, thecal and granulosa cells of follicles or endothelial cells of blood vessels in both ovaries. In the LPS-infused ovary, the activity of this enzyme was higher (P < 0.05 - 0.01) in thecal and granulosa cells of follicles and in endothelium of the blood vessels as compared to that found in the corresponding structures of the contralateral ovary. The highest activity of NADPH-d was observed in luteinizing thecal and granulosa cells of cysts which occured in both ovaries only after LPS infusions. In all the structures of both ovaries, the intensity of NADPH-d reaction was higher (P < 0.05 - 0.001) in LPS-treated gilts than that found in the control group. Light microscopic observations revealed the strongest immunostaining for iNOS in the structures of LPS-infused ovary. In the contralateral ovary, iNOS im- munoreactivity was weaker but still stronger than that found in the control group. This study revealed that in gilts infusions of LPS into the hilus of one ovary enhanced the activity of NADPH-d and iNOS in both the ovaries. However, the activity of these enzymes depended on the location of LPS infusions. The data obtained indicate also that locally synthesised NO can mediate an inflammatory effect of LPS in the ovaries.
2

Hormonal profile and morphological changes in pig ovaries after intraovarian infusions of Escherichia coli endotoxin

84%
Kucharski J., Jana B., Zezula-Szpyra A.
Polish Journal of Veterinary Sciences
|
2002
|
tom 05
|
nr 1
3

Effect of unilateral, intraovarian infusions of bacteria on ovarian morphology in gilts

67%
Jana B., Andronowska A., Kucharski J.
Folia Morphologica
|
2002
|
tom 61
|
nr 1
31-35
The aim of this study was to investigate whether unilateral, intraovarian infusions of bacteria might have induced morphological changes in the contralateral ovary. Eleven sexually matured gilts with controlled estrous cycle were used. The animals were randomly divided into two groups: I (Gr. I, treated; n = 4), and II (Gr. II, control; n = 7). In Gr. I, 1 ml of bacterial suspension (103 colony forming units/ml of saline of Escherichia coli, Staphylococcus aureus and Corynebacterium pyogenes, in proportion 1:1:1) was infused into the hilus of one ovary from the 15th to the 19th day of the estrous cycle. At the same time, 1 ml of saline was infused into the hilus of the contralateral ovary and into both ovaries of the control gilts. On the 7th day of the next cycle, the ovaries were dissected out. There were no significant differences in the number of follicles and corpora lutea (CL) as well as in weight and size between the bacteria-infused, contralateral and control ovaries. The microscopic observations of the bacteria-infused ovaries revealed the presence of focal infiltrations of neutrophils in the softened stroma, especially around dilated blood vessels filled with erythrocytes. In the contralateral ovaries, the number of regularly distributed neutrophils in the softened stroma was greater than that found in the bacteria-treated ovaries. CL of the bacteria-infused ovaries had more numerous, dilated blood vessels than CL observed in the contralateral gonads. More neutrophils were found in CL of both ovaries in Gr. I as compared to those observed in Gr. II. In Gr. II, single neutrophils were found also in the stroma where the tip of the cannula was inserted. This study revealed that in gilts, unilateral, intraovarian administration of bacteria did not change the number of ovarian structures, the weight and size of the bacteria-infused and contralateral ovary, but induced inflammatory changes in both ovaries.
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