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Gene pool variation of twenty varieties and breeding clones of Fragaria × ananassa, nine varieties and breeding lines of Fragaria vesca, and one new interspecific hybrid designated Fragaria × anavesca was analysed with three DNA marker systems. ISSR reactions with four primers produced 45 polymorphic markers. Similarly, RAPD analyses with three primers produced 26 markers and SSR method with three primer pairs revealed 28 different alleles. The total number of 99 polymorphic markers allowed distinguishing clearly a group of F. × ananassa genotypes from that of F. vesca genotypes with F. × anavesca in between of these two. RAPD markers proved to be more informative than ISSRs as 3 of 26 were specific to F. × ananassa only and one exclusively to F. vesca and F. × anavesca. Thus, the presumed hybrid nature of F. × anavesca was effectively confirmed by RAPD markers. Especially important was the 1100bp long PCR product of the B104 primer present in all F. vesca genotypes as well as in F. × anavesca but absent in F. × ananassa. Presence of F. vesca DNA in the hybrid F. × anavesca was additionally corroborated by the 223bp product of the UDF017 primer pair and the 185bp-long band generated with the UDF006 primer pair.
The height growth dynamics of three interspecific crosses with Greek fir (Abies cephalonica Loud.) acting as mother and a control variant from self-pollination were followed throughout a period of 30 years. The hybrid combination A. cephalonica × A. nordmanniana exhibited enhanced growth relative to the selfed control at stage of 4-year old seedlings already, whereas the hybrid A. cephalonica × A. alba only at stage of 9-year old saplings. At age of 30, the heterotic growth persisted only in the latter, the hybrid A. cephalonica × A.nordmanniana was comparable in height growth parameters with a selfed control. The interspecific cross A. cephalonica × A. numidica was remarkable by its accelerated growth during early stages of the development. At age of 4 years it has surpassed in height growth both A. cephalonica × A. alba and control but has declined profoundly during subsequent stages of its development. A conspicuous feature of the hybrid needle anatomy were abundant resin canals and reduced size of some anatomical traits of its needles. In spite of variable growth potentials, all the three interspecific hybrids are recommended for planting in Slovakia, especially at densely polluted areas where domestic silver fir cannot withstand emission pressure.
16-chromosome forms of red clover (2n=14+2) were crossed to six Trifolium species with the chromosome number 2n=16. Hybrid plants were derived from the cross of a stable 16-chromosome red clover T. pratense with T. diffusum (2n=16). No seeds were obtained from reciprocal crosses. F₁ hybrid plants were morphologically more similar to T. diffusum, whereas their other characters, e.g. flower number per head, were intermediate between the species crossed. All F₁ hybrids had the chromosome number 2n=16. Meiosis proceeded with large irregularities. The average number of bivalents per cell was 3.32, and that of univalents - 9.98. Univalents exhibited a high stickiness and frequently formed "end-to-end" configurations and chains consisting of about a dozen of so chromosomes. Bivalents were straight. Lagging chromosomes and chromosome bridges were observed during AI; lagging chromosomes were also found during AII. After an equalizing division, tetrads and different from them microspore polyads were formed. 16-chromosome hybrids were male- and female-sterile. No hybrids were obtained from the stable 16-chromosome red clover T. pratense (n=7 chromosomes) crossed to the selected clover species (T. apertum Bobr., T. alexandrinum L. and others) with n=8 chromosomes.
After duplicating the chromosome number by colchicine in sterile F₁ 16-chromosome hybrid T. pratense × T. diffusum some partially fertile plants with 32 chromosomes were found. Male fertility (viability of pollen grain) was from 69.3% to 86.2% (on average 81.8%), whereas female fertility estimated as seed setting after cross- and self-pollination was 21.8% and 6.9%, respectively. Male and female fertility as well as somatic chromosome number were examined in the F₂-F₄ generations. Selection for female fertility resulted in increasing seed setting in the first two generations (F₂ and F₃) and in decreasing it in F₄ generation. An average seed setting in the F₂-F₄ generations after cross-pollination amounted to 22.2%, 43.6% and 12.9%, respectively; after self-pollination it was 25.2%, 27.6% and 1.9%. In the F₂ generation all the plants had 2n=32 chromosomes. In the next generations there appeared aneuploids, among which 30-chromosome individuals were predominant.
In the years 1964-1994 an extensive programme of wide hybridization within Ihe. Lolium-Festuca complex was carried out in Poland. Six Lolium (ryegrass) and five Festuca (fescue) species at different ploidy level were used for crosses. Hybrids were obtained from 72 cross combinations: 19 interspecific (five Lolium × Lolium and 14 Festuca × Festuca), 51 intergeneric (39 Lolium × Festuca and 12 Festuca × Lolium) and two trispecific (Lolium × Festuca × Festuca), most of them being derived from crosses of four important forage grass species: Lolium multiflorum, L. perenne, Festuca pratensis and F. arundinacea. Interspecific and intergeneric Lolium-F estuca hybrids from 50 cross combinations are maintained at present in the collection of the Institute of Plant Genetics in Poznań. This article presents a complete list of Lolium-Festuca hybrids obtained in Poland in the years 1964-1994 and maintained in the collection. The available literature concerning these hybrids is cited.
The investigated material involved three pungent soft-flesh forms selected from a Capsicum frutescens L. x C. annuum L. hybrid. Capsaicinoid contents, determined by the HPLC technique in the fruit pericarp, amounted to 100 mg. kg-1. The anthers were cultured in vitro employing the method described by Chambonnet (1988) for C. annuum L. with modification involving an increase in kinetin content in R1 medium to 0.2 mg. dm-3. The efficacy of embryogenesis ranged between 0.25% and 4.12%, related to the ratio of the number of obtained plants to the number of cultured anthers, depending on genotype and the employed medium. Conversion of the embryos into plants and the general efficacy of androgenesis were highest in one line. In all of them, cytometrically determined DNA content in cells of leaves in the obtained regenerates amounted 1C.
Otrzymywanie mieszańców międzygatunkowych w rodzaju Lupinus należy do trudnych zadań. Przez wiele lat podejmowano próby przełamania barier krzyżowalności między gatunkami L. albus x L. mutabilis. W wyniku tych prac otrzymano niewiele nasion z niektórych kombinacji krzyżówkowych. Rośliny mieszańcowe rozmnożono i oceniono w kolejnych generacjach. Celem porównania mieszańców międzygatunkowych między sobą i gatunkami rodzicielskimi przeprowadzono ocenę materiału pod względem wybranych cech ilościowych. Materiał badawczy stanowiło dziewięć mieszańców na poziomie pokolenia F6 i F7 i dziesięć form rodzicielskich (L. mutabilis XM-5, LM-13, LM-34, Mut-45, MUT.136, MUT-628, L. graecus, L.vavilovi, L.termis, L. albus ‘WAT’). Na podstawie przeprowadzonej analizy wariancji stwierdzono istotne zróżnicowanie badanych materiałów dla wszystkich analizowanych cech. Biorąc pod uwagę badane cechy sześć mieszańców — (L. termis x L. mutabilis Mut-628), (L. termis x L. mutabilis KW), (L. vavilovi x L. mutabilis Mut.45), (L. vavilovi x L. mutabilis LM.34), (L. mutabilis XM.5 x L. vavilovi), (L. mutabilis LM.13 x L. graecus) było zbliżonych do form matecznych a dwa mieszańce — (L. albus ‘WAT’ x L. mutabilis Mut.136), (L. termis x L. mutabilis XM.5) do form ojcowskich. Masą tysiąca nasion wyróżniły się cztery mieszańce (L. termis x L. mutabilis Mut. 628, L. termis x L. mutabilis KW, L. vavilov x L. mutabilis Mut.45, i L. vavilovi x L. mutabilis LM-34), które zaliczono do tej samej grupy jednorodnej o masie 1000 nasion powyżej 300 g.
Near-amphihaploid hybrid plants of Nicotiana tabacwn x N. alata disomic for some N. alata chromosomes were nearly completely self- and cross-sterile but under open pollination conditions occasionally set poorly filled seed capsules containing very small numbers of seeds. Six plants from those seeds which survived to maturity resembled N. alata. Upon cytological examinations five plants were found to have the diploid chromosome complement of N. alata (2n=18), which suggested their androgenetic origin following outcrossing with paternal N. alata. The sixth plant had 9 bivalents and 11 univalents, which was indicative of its hybrid origin. Unlike the self-incompatible N. alata parent, N. alata-like hybrid derivatives were self-compatible. Surprisingly, the selfed progeny of the 18-chromosome plants showed sensitivity to tentoxin, a chloroplast trait characteristic of paternal N. alata but hardly compatible with its cytoplasmic lineage since the tentoxin-insensitive N. tabacum was the cytoplasmic parent of the hybrid. The progeny of the 29-chromosome plant was tentoxin-insensitive, which agreed with the parentage of the hybrid. Populations derived from the 18-chromosome plants were highly uniform but differed sharply from one another. The offspring of the 29-plant was highly heterogeneous mainly because of univalent segregation in that progeny.
Soft-flesh fruit can be used to produce juices or pastes obtained by mechanical separation of the soft tissue of pericarp from inedible parts. Genotypes selected from interspecific hybrids of Capsicum frutescens L. and Capsicum annuum L. were used as the research material. A general assessment of technological usefulness of the fruit was carried out, and as a result it was noted that there was some diversity in the sizes of the ripe fruit, their mean weight and wall thickness. Significant differences were observed in biotechnological performance – understood as the amount of pericarp separated from inedible parts, namely the placenta with seeds, the remnants of internal partitions and the peel. An HP LC analysis was carried out in order to determine the capsaicin and dihydrocapsaicin contents. The genotypes under examination differed in terms of the contents of capsaicinoids, as well as their ratios. The average values for genotype groups were comparable, and capsaicin and dihydrocapsaicin participation was similar. Considering the amounts of capsaicinoids, the genotypes with the highest content of them can be classified as mildly piquant.
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