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In the present study we analysed the mitochondrial intraspecific variation in natural populations of Norway spruce. We used the second intron of the nad1 gene, which contains two polymorphic short tandem repeats. Due to the maternal inheritance of mitochondrial DNA in Norway spruce, the spatial distribution of haplotype DNA allows insights into seed dispersal and artificial seed transfer. A total of 504 trees distributed all over Austria were genotyped, and 9 different haplotypes could be found. A geographical map of the haplotype variation pattern of Picea abies is presented.
In connection with the decline of spruce stands on the Krynica plots of IPTNS-IUFRO 1964/68, observed in the last decade and attributed to the so-called spiral disease, an attempt was made to determine whether the current health condition of trees is influenced by genotype (provenance). As shown by preliminary observations, the spruces differed in the degree of survival depending on provenance. The inventories of died or broken trees to be removed during the sanitary cutting, made in the years 2000, 2003, 2004 and 2008, yielded also information about the number of spruces that remained in the blocks of the experiment for each provenance and provenance region by Krutzsch (1968). Analysis of variance showed that genotype (provenance) has a statistically significant effect on the survival of trees. In the years 1999-2008, spruces from region 21 (Bohemian Forest) displayed the poorest survival rate in the conditions of the Beskid Sądecki Mts., while those from region 80 (Eastern Siberia) survived best.
The study investigates the intra-population variability of the growth and quality traits of Norway spruce populations from Bliżyn and Zwierzyniec Lubelski. The two populations are included in the IUFRO 1972 experiment and exhibit a high growth dynamics and a narrow crown form. The research was carried out on the experimental plot established in 1996 in Chrosno (Kutno Forest District) using 8228 two-year-old seedlings planted in a single-tree plot design at a spacing of 1.5 × 1.5 m. The seedlings represent 191 open-pollinated families, among them 93 families of Norway spruce from the Bliżyn region and 98 families from the Zwierzyniec Lubelski region. The height, height increment and DBH of trees were measured periodically, and some quality traits were assessed. The last results come from the measurements done on the trees aged 10 years. It was found that the differences between the two populations of Norway spruce are statistically nonsignificant but both of them show wide within-population variability. This suggests that they have the potential to flexibly respond to future changes in the growth conditions or to seed transfer to other seed zones. Survival in the environmental conditions of the experiment was independent of family.
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Quality assessment methods for Picea abies seeds

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The quality assessment of spruce seeds consists mainly in determining their germination capacity, including germination rate (speed), but also purity and weight. Internationally recognised methods are listed in the ISTA Rules for Seed Testing which also specify test conditions. Some countries such as Poland use modified methods. Apart from providing information about the purity of a species, purity testing can also identify the proportion of winged seeds in each seed lot. Germination tests yield data on the maximum germination potential of a seed lot which are used to compare the quality of different lots and to establish the field sowing values. This paper presents the methods used for assessing the quality of Norway spruce seeds, and discusses their advantages and disadvantages and the problems that need further investigation.
The work outlines the directions in the past and present research into the variability of Norway spruce in Poland, and presents the results of provenance experiments concerning the genetic and breeding value of provenances tested in the Polish range of spruce distribution. The Istebna race proved to have a good value, however, so far only the progeny of single stands have been tested. It is thus necessary to determine the range of distribution for this race. The altitude of the location of mother stands significantly correlates with the genetic and breeding value of their progeny cultivated at different altitudes. This suggests that the altitude and exposure of plantations to be established in mountain forest belts should be specified for individual provenances (altitudinal zoning). New inventory provenance tests should be run under varied site conditions to assess both the genetic value and plasticity range of the provenances. In view of the biotic and abiotic threats facing spruce, there is a need to work out detailed programmes designed to preserve its genetic resources in gene banks and in vivo archives.
Over 50 populations of Norway spruce from Bavaria were analysed at 23 isozyme gene loci. The mean genetic distances between these populations were quite small. A geographical grouping could not be observed, and discrimination between provenances from high and low altitudes was not identifiable using this marker type, either. The only difference between spruce populations from South Bavaria and those from Northeast Bavaria is in the presence of some distinct rare alleles. The highest values for the genetic diversity were detected for spruce stands in Northeast Bavaria (Frankonian Forest). Using STS markers, further genes of the nuclear genome of Picea abies can be dealt with. The genetic differences found on the basis of ten STS markers between different Picea abies seed lots and/or seedling populations are generally 2-3 times greater than those found by means of isozyme gene markers. DNA markers turned out to be an appropriate and substantial addition or even more a suitable alternative to isozyme markers for analysing genetic variation and testing provenance identity. Their advantages consist in a markedly wider variation as well as in the enlarged genome segments investigated.
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